Liquid and Frozen Storage of Agouti (Dasyprocta leporina) Semen Extended with UHT Milk, Unpasteurized Coconut Water, and Pasteurized Coconut Water

Abstract

This study evaluated the effects of semen extension and storage on forward progressive motility % (FPM%) in agouti semen. Three extenders were used; sterilized whole cow's milk (UHT Milk), unpasteurized (CW) and pasteurized coconut water (PCW), and diluted to 50, 100, 150, and 200 × 106 spermatozoa/ml. Experiment 1: 200 ejaculates were extended for liquid storage at 5∘C and evaluated every day for 5 days to determine FPM% and its rate of deterioration. Experiment 2: 150 ejaculates were extended for storage as frozen pellets in liquid nitrogen at −195∘C, thawed at 30∘ to 70∘C for 20 to 50 seconds after 5 days and evaluated for FPM% and its rate of deterioration. Samples treated with UHT milk and storage at concentrations of 100 × 106 spermatozoa/ml produced the highest means for FPM% and the slowest rates of deterioration during Experiment 1. During Experiment 2 samples thawed at 30∘C for 20 seconds exhibited the highest means for FPM% (12.18 ± 1.33%), 85% rate of deterioration. However, samples were incompletely thawed. This was attributed to the diameter of the frozen pellets which was 1 cm. It was concluded that the liquid storage method was better for short term storage.

Ultrastructural features of agouti (Dasyprocta aguti) preantral follicles cryopreserved using dimethyl sulfoxide, ethylene glycol and propanediol

“…Therefore, obtaining information regarding reproduction of this species will also provide information for their rational production and consequent preservation [11]. In this regard, several studies have been performed involving the reproductive characteristics of agoutis, such as placentation [10], morphology of the ovaries [12] and oviducts [13], semen collection, and semen cryopreservation [14,15].…”

Preservation Effect of Crude Fig Fruit Filtrate (Ficus carica L) Added In to Tris Egg Yolk Based Extender on Capacitating, Acrosome and Fertility of Half Blood Boer Buck Spermatozoa

“…Each aliquot of semen was slowly added by one half of experimental extender. The other half was added 20 minutes later to minimize osmotic shock (12). To minimize cold shock, those four liquid experimental semen were placed into a 20 ml beacker glass containing 50 ml water at 10 -21°C then keep it in refrigerator.…”

Interactions between Straw Size and Thawing Rates on the Cryopreservation of Agouti (Dasyprocta aguti) Epididymal Sperm

“…Despite this value being twice higher than that verified for ejaculated sperm (12% motile sperm) cryopreserved in UHT milk and coconut water extenders (Mollineau et al 2011), it is hypothesized that agouti presents species-specific sensitivity to the sperm freezing procedures.…”

“…Recently, the cryopreservation of agouti ejaculated sperm was reported (Mollineau et al 2011). Also, a technique to obtain epididymal sperm in this species was described (Ferraz et al 2011), but no protocol for the conservation of this valuable genetic material is available for agoutis.…”

“…The agouti (Dasyprocta agouti) is a terrestrial rodent that inhabits the Neotropical forests and plays an important role in nature, contributing to soil aeration and seed dispersal (Pimentel and Tabarelli 2004). Recently, the cryopreservation of agouti ejaculated sperm was reported (Mollineau et al 2011). Also, a technique to obtain epididymal sperm in this species was described (Ferraz et al 2011), but no protocol for the conservation of this valuable genetic material is available for agoutis.…”