Liquid-chromatographic analysis for urinary porphyrins.

Ford, Roddey E.; Ou, Chin-Nan; Ellefson, Ralph D.

doi:10.1093/clinchem/27.3.397

Cited by 77 publications

(14 citation statements)

References 20 publications

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“…Urinary porphobilinogen was measured spectrophotometrically after complexation with Erlich's reagent by a modification of the method of Schwartz et al (24). Quantitative porphyrins were analyzed using high performance liquid chromatography (25).…”

Section: Methodsmentioning

confidence: 99%

A morbidity study of former pentachlorophenol-production workers.

Hryhorczuk

Wallace

Persky

et al. 1998

Environ Health Perspect

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Pentachlorophenol (PCP) is a pesticide that was once widely used for wood preservation. Commercial PCP contained impurities including higher chlorinated dibenzo-p-dioxins (CDDs) and chlorinated dibenzofurans (CDFs). We investigated the effects of occupational exposure to PCP and its CDD and CDF contaminants on the skin, liver, porphyrin metabolism, and central and peripheral nervous systems. In 1986 we conducted a medical survey of 366 workers who had been engaged in the production of PCP at a single plant between 1938 and 1978. The referent group consisted of 303 workers from the same plant who were not exposed to these or related compounds. Exposure was determined from computerized personnel records. The medical survey included an administered questionnaire, medical record review, physical examination by dermatologists, internists, and neurologists, and analysis of 24-hr urine for quantitative porphyrins among other tests. In this paper we present the results of analyses of the general health, chloracne, and porphyrin metabolism end points. The general health status of PCP workers was similar to unexposed workers, but 17.8% of PCP workers had evidence of current or past chloracne. PCP workers with chloracne had significantly higher mean urinary excretion of coproporphyrins (117. 0 vs. 90.6 microg/24 hr) than unexposed workers after controlling for potential confounders. Workers with chloracne who had worked with both PCP and polychlorinated biphenyls had significantly higher mean urinary excretions of hepta-, penta-, and coproporphyrins than unexposed workers. We conclude that occupational exposure to PCP is associated with chloracne and biochemical abnormalities which may persist years after exposure.

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Section: Methodsmentioning

confidence: 99%

A morbidity study of former pentachlorophenol-production workers.

Hryhorczuk

Wallace

Persky

et al. 1998

Environ Health Perspect

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“…Reversed-phase HPLC systems utilising gradient phosphate buffers combined with acetonitrile have been developed for the separation of porphyrins Abe and Konaka, 1989;Lin and Huie, 1997). Similarly, reversed-phase HPLC methods using methanol and phosphate buffers for the separation (Ford et al, 1981;Schreiber et al, 1983;Gederaas et al, 2000). Porphyrin acids have been separated using the ion-pairing agent, tetrabutylammonium phosphate, together with a ternary mobile phase system (Bonnet et al, 1978).…”

Section: Porphyrinsmentioning

confidence: 99%

Liquid chromatography and mass spectrometry of haem biosynthetic intermediates: a review

Benton

Lim

2012

Biomedical Chromatography

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This article discusses the separation, analysis and characterisation of intermediates and oxidative by-products of the haem biosynthetic pathway by liquid chromatography and mass spectrometry. Techniques reviewed include high-performance liquid chromatography, ultra-high-performance liquid chromatography, capillary electrophoresis, ion mobility spectrometry, mass spectrometry and tandem mass spectrometry. The emphasis was on the analysis of biological and clinical samples.

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“…A 20 ,Al aliquot of each HCI fraction was analysed by h.p.l.c. [36] using a Micropak SP-C18-5 column (15 cm x 4.6 mm int. diam.…”

Section: Urine Collection and Porphyrin Analysismentioning

confidence: 99%

“…H.p.l.c. solvents were as described by Ford et al [36] and were degassed with helium immediately before use. By using this method, recovery of porphyrins added to control urine samples was 94 + 4 %.…”

Section: Urine Collection and Porphyrin Analysismentioning

confidence: 99%

The role of the Ah locus in hexachlorobenzene-induced porphyria. Studies in congenic C57BL/6J mice

Hahn

Gasiewicz

Linko

et al. 1988

Biochemical Journal

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The role of the Ah locus in hexachlorobenzene (HCB)-induced porphyria and the possible involvement of P-450 cytochromes P(1)450 and P(3)450 in the pathogenesis of this disease were investigated in two congenic strains of C57BL/6J mice that differ only at this locus. Female B6-Ahb mice (Ah receptor: approximately 30-70 fmol/mg of cytosolic protein) and B6-Ahd mice (Ah receptor: undetectable) were pretreated with iron (500 mg/kg) and then fed a diet containing 0 or 200 p.p.m. of HCB for up to 17 weeks. Mice from the two strains consumed similar amounts of HCB. Urinary excretion of porphyrins was increased after 7 weeks of HCB treatment in B6-Ahb mice, and after 15 weeks was over 200 times greater than that of mice given iron only. In B6-Ahd mice, porphyrin excretion did not begin to increase until after 13 weeks, and after 15 weeks was only six times greater than that of controls. Similar differences were seen in the 15-week hepatic porphyrin concentrations (B6-Ahb: 1110 +/- 393; B6-Ahd: 17.6 +/- 14.5; controls: approximately 0.20 nmol/g). Uroporphyrinogen decarboxylase (EC 4.1.1.37) activity was diminished by 70 and 20% in B6-Ahb B6-Ahd mice respectively after 15 weeks of treatment with HCB. Cytochromes P(1)450 and P(3)450 were measured in hepatic microsomes (microsomal fractions) by radioimmunoassay and immunoblotting, using antisera raised against the orthologous rat isoenzymes P450c and P450d. HCB induced small amounts of a protein recognized by anti-P450c (P(1)450) in B6-Ahd mice, but not in B6-Ahd mice. Relatively large amounts of a protein recognized by anti-P450d (P(3)450) were induced in both strains, but to a somewhat greater extent in the B6-Ahb mice. The hepatic accumulation of HCB at 15 weeks was greater in B6-Ahb than in B6-Ahd mice, in association with elevated hepatic lipid levels in the former strain. The results of this experiment indicate that the Ah locus influences the susceptibility of C57BL/6J mice to HCB-induced porphyria and are consistent with the suggestion that the sustained induction of P(3)450 and/or P(1)450 may be a causative factor in the development of this disease.

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Liquid-chromatographic analysis for urinary porphyrins.

Cited by 77 publications

References 20 publications

A morbidity study of former pentachlorophenol-production workers.

A morbidity study of former pentachlorophenol-production workers.

Liquid chromatography and mass spectrometry of haem biosynthetic intermediates: a review

The role of the Ah locus in hexachlorobenzene-induced porphyria. Studies in congenic C57BL/6J mice

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