Liquid Chromatographic and Spectral Analysis of the 17-Hydroxy Anabolic Steroids

Abstract

The liquid chromatographic properties of various 17-hydroxy anabolic steroids are examined under reversed-phase conditions. These anabolic steroids are now listed as controlled drugs in many states due to their abuse potential in athletics, body building, and other areas. These nonesterified steroids are separated on a C18 stationary phase with a 70% methanol in water mobile phase. In a few cases, two compounds display very similar retention properties. However, dual-wavelength detection at 254 and 280 nm allows for their differentiation. Reversed-phase retention parallels steroid lipophilicity based on hydroxyl and methyl group substituents. Also, those steroids containing a dienone substructure are more polar than steroids containing an enone moiety.

Voltammetric study of danazol and its determination in capsules and spiked biological fluids

“…A quantitative TLC method has been reported for the validation of the purity analysis of DZ and its capsules [5]. HPLC has been frequently applied to the analysis of DZ in bulk form [6] pharmaceutical formulations [7][8][9][10][11] and biological fluids [11][12][13][14][15][16][17]. Gas chromatography [18,19] and immunoassay [20,21] was also reported.…”

Square-wave adsorptive stripping voltammetric determination of danazol in capsules

“…However, most of the reported methods for the analysis of danazol rely deeply on the use of chromatographic techniques. HPLC [11][12][13][14][15][16][17], GC [18,19] and TLC [20] chromatographic procedures have been described for identification and determination of danazol. However, although danazol predominantly analysed and studied by these chromatographic approaches, yet other analytical techniques such as chemiluminescent immunoassay [21] voltammetry [22] were also applied for similar analytical task.…”

Ion trap mass spectrometry for the characterization of N-methyl-1-(3,4-methylenedioxyphenyl)-2-butanamine and N-ethyl-3,4-methylenedioxyamphetamine, two widely distributed street drugs

“…In fact the EI mass spectrum of MBDB is practically superimposable on those of MDEA and other isomeric amphetamines, so that EI mass spectrometry can not be successfully employed for its identification. 1,3 Only tandem mass spectrometric experiments (MS/MS), 4 performed using a triple quadrupole instrument on the fragment ions at m/z 72, can lead to a clear distinction of MBDB vs. MDEA [5][6][7] without the use of derivatization techniques.…”