Liquid chromatographic determination of ammonium in water

Mizobuchi, Munehiko; Tamase, Kikuo; Kitada, Yoshimi; Sasaki, Michiko; Tanigawa, Kaoru

doi:10.1021/ac00267a073

Cited by 4 publications

(5 citation statements)

References 16 publications

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“…The determination was performed by reversed-phase chromatography with fluorescence detection after derivatization with fluorescamine using 1-butylamine as an internal standard (Mizobuchi et al, 1984).…”

Section: Smoke Chemistrymentioning

confidence: 99%

Reduced exposure evaluation of an Electrically Heated Cigarette Smoking System. Part 2: Smoke chemistry and in vitro toxicological evaluation using smoking regimens reflecting human puffing behavior

Zenzen

Diekmann

Gerstenberg

et al. 2012

Regulatory Toxicology and Pharmacology

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Chemical analysis of up to 49 harmful and potentially harmful constituents (HPHC) in mainstream smoke, in vitro cytotoxicity of the particulate and gas/vapor phase of mainstream smoke determined in the Neutral Red Uptake assay, and in vitro bacterial mutagenicity of the particulate phase determined in the Salmonella typhimurium Reverse Mutation (Ames) assay are reported for three Electrically Heated Cigarette Smoking System (EHCSS) series-K cigarettes, the University of Kentucky Reference Cigarette 2R4F, and a number of comparator commercial conventional lit-end cigarettes (CC) under ISO machine-smoking conditions and a total of 25 additional smoking regimens reflecting 'human puffing behavior' (HPB). The smoking machines were set to deliver nicotine yields for the EHCSS and comparator CC derived from the 10th percentile to the 90th percentile of nicotine uptake distributions in smokers determined in two clinical studies. Duplication of the smoking intensity 'per cigarette' on a smoking machine may provide an insight into product performance that is directly relevant to obtaining scientific evidence for reduced exposure substantiation based on mainstream cigarette smoke HPHC-to-nicotine regressions. The reported data support an overall evaluation of reduced exposure to HPHC and biological activity.

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Section: Smoke Chemistrymentioning

confidence: 99%

Reduced exposure evaluation of an Electrically Heated Cigarette Smoking System. Part 2: Smoke chemistry and in vitro toxicological evaluation using smoking regimens reflecting human puffing behavior

Zenzen

Diekmann

Gerstenberg

et al. 2012

Regulatory Toxicology and Pharmacology

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show abstract

“…The derivatization conditions were adapted from the literature , , , , , notably by adjusting the buffering conditions to the smoke matrix. Cigarette mainstream smoke samples were collected with a combination of a Cambridge filter pad and an impinger filled with 25 mL of an aqueous sulfuric acid solution, on the basis of a modified version of the Health Canada method .…”

Section: Resultsmentioning

confidence: 99%

“…Precolumn derivatization techniques for amino acids, amines, and peptides are numerous and offer several advantages, notably simple procedures, increased sensitivity, and reactions that are quick, quantitative, and reproducible − . Fluorescamine and dansyl chloride (DNS) have both been successfully used for the quantitation of ammonia or other amino acids in various matrices such as water − , urine − , air , and plant and animal tissues , . Therefore, the objectives of the present study were to evaluate these two derivatization reagents with the aim of increasing reliability, robustness, selectivity, and sensitivity of the commonly used methods.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Two Derivatization Reagents for the Determination by LC-MS/MS of Ammonia in Cigarette Mainstream Smoke

Mottier

Jeanneret

2010

J. Agric. Food Chem.

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Ammonia in cigarette mainstream smoke was quantified by LC-MS/MS after derivatization. Two different reagents, fluorescamine and dansyl chloride, were investigated, but only the latter gave stable derivatives; therefore, it was considered the most appropriate choice. Smoke samples were collected on a Cambridge filter pad followed by an impinger containing a solution of hydrochloric acid. Ammonia was then derivatized with a 18.5 mM solution of dansyl chloride in acetonitrile at 70 °C for 30 min in a vial with the internal standard, (15)ND(4)Cl. The resulting derivative was analyzed by LC-MS/MS detection with an atmospheric pressure chemical ionization (APCI) interface in the positive ionization mode using multiple-reaction monitoring (MRM). Good linearity was obtained in the concentration range of 0.02-1.65 μg/mL (r(2) ≥ 0.999), and the limit of detection (LOD) was established at 0.006 μg/mL. This method has the advantage of being sensitive, efficient, and reliable and is not hindered by interferences from the sample matrix. It should thus be considered a reference method of choice for the determination of ammonia in smoke.

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“…Mizobuchi et al 81 have described a method for the determination of ammonia in water based on reaction with fluorescamine to form a fluorophore, which is then chromatographed on trichrosorb RP-18 using a mobile phase consisting of 0.05 m phosphate buffer solution (pH 2.0) and acetonitrite (65:25 v/v). Average recoveries were from 95.8% to 100.7% and their standard deviations were from 1.9 to 5.4.…”

Section: Ion Chromatographymentioning

confidence: 99%

“…81 Conboy et al 82 employed ion chromatography coupled with a mass spectrometric detector for the determination of ammonium ions (and sulfate) in natural waters.…”

Section: Ion Chromatographymentioning

confidence: 99%

Metals in River Water

Crompton

2015

Determination of Metals in Natural Waters, Sediments and Soils

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Liquid chromatographic determination of ammonium in water

Cited by 4 publications

References 16 publications

Reduced exposure evaluation of an Electrically Heated Cigarette Smoking System. Part 2: Smoke chemistry and in vitro toxicological evaluation using smoking regimens reflecting human puffing behavior

Reduced exposure evaluation of an Electrically Heated Cigarette Smoking System. Part 2: Smoke chemistry and in vitro toxicological evaluation using smoking regimens reflecting human puffing behavior

Evaluation of Two Derivatization Reagents for the Determination by LC-MS/MS of Ammonia in Cigarette Mainstream Smoke

Metals in River Water

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