Liquid Chromatographic Determination of Five Benzoylurea Insecticides in Fruit and Vegetables

Gamón, M.; Saez, A.; Pelegri, R.; Peris, I; Cuadra, José García de la; Coscolla, R.

doi:10.1093/jaoac/81.5.1037

Cited by 26 publications

(4 citation statements)

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“…UHPLC system Dionex UltiMate 3000 UHPLC systems GmbH, Germany with photodiode array detector as described by Gamon et al (1998) with the following conditions, mobile phase for isoproturon and metribuzin was methanol: water (90/10 v/v) at a flow rate of 1.0 mL/min with detection wavelength was set at 210 nm and retention time was about 3.31 and 3.76 min, respectively.…”

Section: Apparatusmentioning

confidence: 99%

Effect of Storage Temperature on Degradation of Two Herbicides and Their Sub-Acute Toxicity on Albino Rats

Khozimy¹,

Ramadan²,

Shaldam³

et al. 2017

Journal of Applied Plant Protection

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Two different herbicides isoproturon and metribuzin were stored at zero °C for 7 days and under accelerated storage temperature at 54°C for 14 days to study degradation of active ingredient which determined by HPLC-DAD. Physical properties such as change of pH values, suspensibility, wet sieve test, pourability, spontaneity of dispersion and persistent foam test before and after storage were also studied according to FAO specification. Results showed that active ingredient of isoproturon formulation was slightly affected by accelerated storage temperature at 54°C and become conformity with FAO specification but metribuzin formulation was affected when stored after 10 days at 54°C while the degradation increasing by long time of storage. Physical properties of two herbicides formulations passed successfully through all storage periods at zero °C for 7 and at 54°C for 14 days except wet sieve test of metribuzin. On the other hand, the present two herbicides were evaluated using 1/20 of their LD 50 on some biochemical components of albino rats in initial time at 23±2°C and after 14 days from storage at 54°C. The results showed that the two herbicides in initial time caused high decreasing in content of total soluble protein with high increasing in levels of glucose, urea, creatinine, bilirubin, cholesterol and triglycerides. Also the activities of Aspartate aminotransferase (AST), Alanine Aminotransferase (ALT) and Alkaline phosphatase (ALP) were high relative to control. In case of two herbicides after storage 14 days at 54°C, the results showed slight decrease in content of total soluble protein, but the levels of serum glucose, urea, creatinine, bilirubin, cholesterol and triglycerides were low increase relative to health normal control. The results showed that activities of AST, ALT and ALP were the stimulated under the effect of the both herbicides compared with the normal. The biochemical harmful of the herbicides under storage at 54°C for 14 days was lower than those of effect initial time at 23±2°C were compare, the influences of metribuzin on the biochemical study it was more than that of isoproturon either in initial time at 23±2°C and after 14 days from storage at 54°C.

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Section: Apparatusmentioning

confidence: 99%

Effect of Storage Temperature on Degradation of Two Herbicides and Their Sub-Acute Toxicity on Albino Rats

Khozimy¹,

Ramadan²,

Shaldam³

et al. 2017

Journal of Applied Plant Protection

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“…The LOQs of the targeted 8 BPUs are significantly lower in this method than those in the previously reported methods, and the percent recoveries are sufficiently high. Regarding organic solvent consumption, only 2 mL of CH 2 Cl 2 is required for extraction and 1 mL of acetonitrile for solvent exchange, compared with the high solvent consumption of the previous methods [8,38,39,[52][53][54][55][56].…”

Section: Comparison Of the Optimized Methods With The Previous Studiesmentioning

confidence: 99%

A vortex-assisted liquid-liquid extraction followed by dispersive-solid phase extraction (VA-LLE/d-SPE) for the determination of eight benzoylphenylureas insecticides in tomatoes and cucumbers

Alhamami,

Algethami,

Ramadan

et al. 2024

AChrom

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Monitoring benzoylphenylureas (BPUs) residues in ready-to-eat vegetables is of great interest for an adequate assessment of human pesticide exposure. A rapid, inexpensive, simple, and effective method for determining 8 BPUs insecticides in tomatoes and cucumbers was developed and validated. Vortex-assisted liquid-liquid extraction (VA-LLE) followed by dispersive solid-phase extraction (d-SPE) using graphitized carbon black (GCB) for cleanup was used before LC-MS/MS analysis. Different parameters were optimized, including the type and volume of extractants, vortex time, and the type and amount of adsorbents used for cleanup. The evaluation showed that the method has excellent linearity (R2 ≥ 0.994). The recovered 8 BPUs insecticides from spiked tomato and cucumber samples at 0.01, 0.05, and 0.25 mg kg−1 ranged from 83.2 to 105.2%, with RSD of 4.9–14.6%. The limits of quantitation (LOQs) were 0.0025 mg kg−1 (0.005 mg kg−1 for lufenuron). Within-day repeatability ranged from 3.9 to 13.9%, while between-day repeatability ranged from 8.9% to 17.7%. The optimized method was used to analyze 100 samples of tomatoes and cucumbers marketed in Saudi Arabia.

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“…Because of their thermolability, benzoylurea residues have generally been analyzed by high-performance liquid chromatography (HPLC) with ultraviolet detector, [8][9][10][11] uorescence detector [12][13][14] or mass spectrometry, [15][16][17][18] etc. Gas chromatography (GC) has scarcely been employed to determine these insecticides, and only diubenzuron and lufenuron were determined by gas chromatography with electron capture detection (GC-ECD).…”

Section: Introductionmentioning

confidence: 99%

Analysis of benzoylurea insecticides in water samples with TiO₂nanotube array micro-solid phase extraction coupled to high performance liquid chromatography

Zhou

Huang

2014

RSC Adv.

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Liquid Chromatographic Determination of Five Benzoylurea Insecticides in Fruit and Vegetables

Cited by 26 publications

References 0 publications

Effect of Storage Temperature on Degradation of Two Herbicides and Their Sub-Acute Toxicity on Albino Rats

Effect of Storage Temperature on Degradation of Two Herbicides and Their Sub-Acute Toxicity on Albino Rats

A vortex-assisted liquid-liquid extraction followed by dispersive-solid phase extraction (VA-LLE/d-SPE) for the determination of eight benzoylphenylureas insecticides in tomatoes and cucumbers

Analysis of benzoylurea insecticides in water samples with TiO₂nanotube array micro-solid phase extraction coupled to high performance liquid chromatography

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Liquid Chromatographic Determination of Five Benzoylurea Insecticides in Fruit and Vegetables

Cited by 26 publications

References 0 publications

Effect of Storage Temperature on Degradation of Two Herbicides and Their Sub-Acute Toxicity on Albino Rats

Effect of Storage Temperature on Degradation of Two Herbicides and Their Sub-Acute Toxicity on Albino Rats

A vortex-assisted liquid-liquid extraction followed by dispersive-solid phase extraction (VA-LLE/d-SPE) for the determination of eight benzoylphenylureas insecticides in tomatoes and cucumbers

Analysis of benzoylurea insecticides in water samples with TiO2nanotube array micro-solid phase extraction coupled to high performance liquid chromatography

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Analysis of benzoylurea insecticides in water samples with TiO₂nanotube array micro-solid phase extraction coupled to high performance liquid chromatography