Liquid Chromatographic Determination of Oxytetracycline in Bovine Plasma by Double-Phase Extraction

Nelis, H. J.; Vandenbranden, J; Kruif, Aart de; Belpaire, Frans; Leenheer, A.P. De

doi:10.1002/jps.2600811218

Cited by 10 publications

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One way of achieving this is to decrease their consumption, and this requires monitoring methods for tetracycline residues in samples such as blood serum, urine, milk, egg, and animal tissues . Tetracycline has been previously determined in blood serum by using liquid chromatography, , high-performance liquid chromatography, − capillary electrophoresis, stopped-flow mixing, and differential-pulse polarography . Very recently, methods based on titrimetry, recombinant Escherichia coli sensors, and electrospray ionization mass spectrometry have also been described for the determination of tetracyclines.…”

mentioning

confidence: 99%

Enhanced Synchronous Spectrofluorometric Determination of Tetracycline in Blood Serum by Chemometric Analysis. Comparison of Partial Least-Squares and Hybrid Linear Analysis Calibrations

1999

View full text Add to dashboard Cite

Tetracycline has been determined in human serum samples by a combination of: (1) synchronous fluorescence spectra of whole sera treated with Mg2+, and (2) the multivariate calibration methods of partial least-squares (PLS-1) and a variant of the recently introduced hybrid linear analysis (HLA), which does not require the knowledge of pure-component spectra. The calibration set was designed with 50 sera spiked with concentrations of tetracycline in the range 0.0-4.0 micrograms mL-1'. Studies concerning validation, precision, accuracy and figures of merit (selectivity, sensitivity and limit of determination) were also carried out. A novel wavelength-selection procedure was applied to minimize the effect of nonmodeled interferents present in serum samples containing bilirubin, triglycerides, and salicylate. Overall, the performance of the newly developed HLA approach seems to be better than that of PLS-1.

show abstract