Liquid Chromatographic Determination of Scopolamine in Hair with Suspended Drop Liquid Phase Microextraction Technique

Abstract

Hair analysis is used in some branches of alternative medicine as a method of investigation to assist diagnosis. It is very useful when a history of drug use is difficult or impossible to obtain. In this research suspended droplet liquid phase microextraction (SDLME) coupled with high-performance liquid chromatography and photodiode array detection (HPLC-DAD) was used for preconcentration and analysis of scopolamine in hair samples. Therefore scopolamine was extracted from 2.0 g hair sample incubated in methanol (5 h, 50˚C) and adjusted to pH 7.4 with, Na 2 HPO 4 -H 3 PO 4 buffer solution (donor phase, P 1 ) into an organic phase (P 2 ) 350 µl n-octanol and then back extracted into a micro drop of aqueous acceptor phase (P 3 ), adjusted at pH 3, with HCL. The extraction time, T 1 (from P 1 to P 2 ) was 2 min and T 2 (from P 2 to P 3 ) was 30 min. Optimum instrumental conditions were included; A C 18 reverse phase column with water-acetonitrilemethanol (80:10:10) as the mobile phase was used and wavelength for UV detection was 205 nm. The linear range was 10 to 10000 ng·mL -1 , enrichment factor, detection limit and relative standard deviation were 77, 0.1 ng·mL -1 and 5.4 respectively.

Improved Analytical Approach for Determination of Tropane Alkaloids in Leafy Vegetables Based on µ-QuEChERS Combined with HPLC-MS/MS

“…Similar works can be found in the literature but involve drug or biological samples. For example, µ-SPE has been applied to determine cocaine and cocaethylene in plasma [ 11 ], solid-phase microextraction (SPME) for the analysis of drugs, including atropine [ 12 ], and the single-droplet, liquid-phase microextraction (SDLME) technique to concentrate scopolamine from hair samples [ 13 ]. In this sense, the application of miniaturized techniques in the analysis of TAs in foods is an unexplored field.…”

Optimization of RP-Gradient-HPLC Method for Evaluation of Impurities of Scopolamine from Transdermal Delivery Patch

“…Few articles were published which describes about extraction and analysis of scopolamine along with its derivatives, tropines from natural sources and medicinal plants using liquid chromatography methods [2][3][4][5][6][7][8][9]. Few works described about analysis of scopolamine and its degradation products by GLC, GC and GC-MS [10][11][12].…”