Liquid chromatographic–fluorimetric method for the estimation of nitric oxide biosynthesis in the central nervous system

Pérez-Neri, Iván; Montes, Sergio; Boll, Marie‐Catherine; Ramı́rez-Bermúdez, Jesús; Rı́os, Camilo

doi:10.1016/j.jchromb.2004.03.043

Cited by 14 publications

(9 citation statements)

References 36 publications

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“…Our results also showed that there was no significant difference in the arginine level between the labor pain and the no pain group. This concurs with the results from Perez-Neri et al [47] where no difference in the CSF arginine levels were observed between healthy human control and patients with infection and inflammatory processes in the CNS, even though both of these conditions are known to be associated with increased NO production. A study applying this very same method for NO analysis in CSF on patients with fibromyalgia has also shown an increase in citrulline level with increasing pain [48].…”

Section: Inhibitory Amino Acidssupporting

confidence: 92%

“…Similar indirect analysis of NO using arginine and citrulline levels in CSF has also been reported by Perez-Neri et al in patients with infections and inflammatory processes in CNS [47]. This report analyzed in detail the validity of analyzing NO using citrulline and arginine levels taking into consideration the effects of other metabolic processes involving these compounds.…”

Section: Inhibitory Amino Acidssupporting

confidence: 70%

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Changes in Amino Acids and Nitric Oxide Concentration in Cerebrospinal Fluid During Labor Pain

et al. 2006

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This study analyzes the relationship between amino acids and pain perception during active labor. Cerebrospinal fluid (CSF) levels of the excitatory amino acids (EAAs)-glutamate, aspartate and their amide forms, inhibitory amino acids (IAAs)-glycine, gamma-amino butyric acid (GABA) and taurine and nitric oxide (NO) related compounds-arginine and citrulline (by-product of NO synthesis) were compared between pregnant women at term pregnancy with labor pain (n = 38) and without labor pain (Caesarian section; n = 30). The levels of aspartate, glycine, GABA and citrulline were significantly higher; whilst taurine was significantly lower in the labor pain group. These findings suggest that aspartate and NO are associated with labor pain. An inhibitory role for the IAA taurine and a pronociceptive role for glycine in labor pain are proposed.

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Section: Inhibitory Amino Acidssupporting

confidence: 92%

Section: Inhibitory Amino Acidssupporting

confidence: 70%

Changes in Amino Acids and Nitric Oxide Concentration in Cerebrospinal Fluid During Labor Pain

et al. 2006

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“…Plasma was obtained by centrifugation (12,000 rpm, 5 min, 4°C) and stored at −80°C until analyzed. Citrulline concentrations were determined using a reverse-phase HPLC-fluorimetry method with precolumn OPA/ME derivatization, as described previously by Pérez-Neri et al (20). …”

Section: Methodsmentioning

confidence: 99%

γ-Tocotrienol Ameliorates Intestinal Radiation Injury and Reduces Vascular Oxidative Stress after Total-Body Irradiation by an HMG-CoA Reductase-Dependent Mechanism

et al. 2009

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Analogs of vitamin E (tocols) are under development as radioprophylactic agents because of their high efficacy and lack of toxicity. Gamma-tocotrienol (GT3) is of particular interest because, in addition to being an antioxidant, it also inhibits 3-hydroxy-3-methylglutaryl-coenzyme A (HMGCoA) reductase and accumulates to greater extent in endothelial cells than other tocols. We addressed in vivo whether HMG-CoA reductase inhibition contributes to the radioprotection conferred by GT3. Groups of mice were treated with vehicle, mevalonate (the product of the reaction catalyzed by HMG-CoA reductase), GT3 alone or GT3 in combination with mevalonate. Lethality and standard parameters of injury to the hematopoietic, intestinal and vascular/ endothelial systems were assessed after exposure to total-body irradiation. GT3 improved postirradiation survival and decreased radiation-induced vascular oxidative stress, an effect that was reversible by mevalonate. GT3 also enhanced hematopoietic recovery, reduced intestinal radiation injury, and accelerated the recovery of soluble markers of endothelial function. These parameters were not reversed by mevalonate co-administration. Our data confirm GT3's radioprophylactic properties against hematopoietic injury and, for the first time, demonstrate benefits in terms of protection against gastrointestinal and vascular injury. The radioprotective efficacy of GT3 against vascular injury is related to its properties as an HMG-CoA reductase inhibitor.

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“…Plasma was generated by centrifugation (12000 rpm, 5 min, 4°C) and stored at −80°C until analysis. Citrulline concentrations were determined using a reverse-phase HPLC-fluorimetry method with precolumn OPA/ME derivatization, as described previously by Pérez-Neri et al (17). …”

Section: Methodsmentioning

confidence: 99%

The Somatostatin Analog SOM230 (Pasireotide) Ameliorates Injury of the Intestinal Mucosa and Increases Survival after Total-Body Irradiation by Inhibiting Exocrine Pancreatic Secretion

Berbée

Boerma

et al. 2009

Radiation Research

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Somatostatin analogs ameliorate intestinal injury after localized irradiation. This study investigated whether SOM230, a novel, metabolically stable analog with broad receptor affinity, reduces intestinal injury and lethality in mice exposed to total-body irradiation (TBI). Male CD2F1 mice were exposed to 7–15 Gy TBI. Twice-daily administration of SOM230 (1, 4 or 10 mg/kg per day) or vehicle was started either 2 days before or 4 h after TBI and continued for either 14 or 21 days. Parameters of intestinal and hematopoietic radiation injury, bacterial translocation, and circulating cytokine levels were assessed. Animal survival was monitored for up to 30 days. SOM230 increased survival (P < 0.001) and prolonged survival time (P < 0.001) whether administration was initiated before or after TBI. There was no benefit from administration for 21 compared to 14 days. The survival benefit of SOM230 was completely reversed by co-administration of pancreatic enzymes (P = 0.009). Consistent with the presumed non-cytoprotective mechanism of action, SOM230 did not influence hematopoietic injury or intestinal crypt lethality. However, SOM230 preserved mucosal surface area (P < 0.001) and reduced bacterial translocation in a dose-dependent manner (P < 0.001). Circulating IL-12 levels were reduced in SOM230-treated mice (P = 0.007). No toxicity from SOM230 was observed. SOM230 enhances animal survival whether administration begins before or after TBI; i.e., it is effective both as a protector and as a mitigator. The mechanism likely involves reduction of intraluminal pancreatic enzymes. Because of its efficacy and favorable safety profile, SOM230 is a promising countermeasure against radiation and should undergo further development.

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Liquid chromatographic–fluorimetric method for the estimation of nitric oxide biosynthesis in the central nervous system

Cited by 14 publications

References 36 publications

Changes in Amino Acids and Nitric Oxide Concentration in Cerebrospinal Fluid During Labor Pain

Changes in Amino Acids and Nitric Oxide Concentration in Cerebrospinal Fluid During Labor Pain

γ-Tocotrienol Ameliorates Intestinal Radiation Injury and Reduces Vascular Oxidative Stress after Total-Body Irradiation by an HMG-CoA Reductase-Dependent Mechanism

The Somatostatin Analog SOM230 (Pasireotide) Ameliorates Injury of the Intestinal Mucosa and Increases Survival after Total-Body Irradiation by Inhibiting Exocrine Pancreatic Secretion

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