Liquid chromatographic method for analysis of saponins in Maesa balansae extract active against leishmaniasis

Leonard, Stefanie; Capote, Raul; Germonprez, Nils; Puyvelde, Luc Van; Kimpe, Norbert De; Vermeersch, Hans; Rosier, Jan; Maes, Louis; Roets, Eugène; Hoogmartens, Jos

doi:10.1016/s0021-9673(03)01172-5

Cited by 10 publications

(6 citation statements)

References 5 publications

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“…The extraction/purification of the active saponins consisted of two major steps: a solvent extraction of the biomass (dried and milled leaves of M. balansae ), leading to “semi-purified” PX-6518, followed by a preparative chromatography leading to purified PX-6518, which actually consisted of a mixture of six saponins called maesabalides I to VI [ 14 ]. The exploratory toxicity studies were performed with purified PX-6518 in the mouse.…”

Section: Methodsmentioning

confidence: 99%

“…In the dog studies, plasma levels of the maesabalide-III and -IV were assessed using liquid chromatography combined with tandem mass spectrometry [ 14 , 32 ]. The analytes were extracted from plasma using acetonitrile (ACN).…”

Section: Methodsmentioning

confidence: 99%

“…Among the wide range of antimicrobial properties that have been reported, including antifungal, antiviral, antibacterial, antiprotozoal, piscicidal, molluscicidal and insect-larvicidal action [ 4 ], the present study specifically focused on the antileishmanial oleane triterpene saponin PX-6518 [ 14 ], which is a crude methanolic extract of the leaves of a Vietnamese plant Maesa balansae (fam. Myrsinacceae ), consisting of six maesabalides for which potent and selective action against different Leishmania species was demonstrated [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Intravenous and Subcutaneous Toxicity and Absorption Kinetics in Mice and Dogs of the Antileishmanial Triterpene Saponin PX-6518

Maes

2013

Molecules

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The intravenous (IV) and subcutaneous (SC) toxicity and absorption kinetics of the antileishmanial triterpene saponin PX-6518 and its active constituents maesabalide-III and -IV were studied in mice and dogs. A high-dose wash-out study of PX-6518 at 20 mg/kg SC for 5 days and a single low-dose wash-out study at 1, 2.5 or 5 mg/kg SC and IV with follow-up until day 35 after treatment were performed in mice. Beagle dogs received three escalating doses of maesabalide-III and -IV at weekly intervals (0.01, 0.1 and 0.5 mg/kg IV and maesabalide-III was also dosed SC at 0.1, 0.2 and 0.4 mg/kg). Endpoint measurements included clinical, hematological and serum biochemical parameters. Pathology and toxicokinetic studies were performed on the dogs. Whereas the neutrophils and aspartate aminotransferase and alanine aminotransferase levels were increased in the high-dose wash-out mouse study, these parameters did not change in the low-dose wash-out study. The dogs were far more susceptible than mice to liver toxicity (hepatocellular necrosis and elevated liver enzymes) and developed a painful inflammatory reaction at the SC injection site. Toxicokinetic analysis revealed a non dose-linear systemic availability with plasma concentrations above the antileishmanial IC50 after only a single dose at 0.01 mg/kg IV or 0.1 mg/kg SC. Related to the long half-life (T1/2 71–91 h after SC dosing), repeated dosing at weekly intervals may result in drug accumulation and enhanced toxicity. It was decided not to pursue further drug development for PX-6518 because of the hepatotoxic risk.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Intravenous and Subcutaneous Toxicity and Absorption Kinetics in Mice and Dogs of the Antileishmanial Triterpene Saponin PX-6518

Maes

2013

Molecules

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“…In general, HPLC can be used to analyze almost all the compounds in the herbal medicines. Thus, over the past decades, HPLC has received the most extensive application in the analysis of herbal medicines [63][64][65][66][67][68][69][70][71][72][73][74][75][76]. Reversed-phase (RP) columns may be the most popular columns used in the analytical separation of herbal medicines.…”

Section: High-performance Liquid Chromatographymentioning

confidence: 99%

“…Thus, a good experimental design for the optimal separation seems in general necessary [73,76]. In order to obtain better separation, some new techniques have been recently developed in research field of liquid chromatography.…”

Section: High-performance Liquid Chromatographymentioning

confidence: 99%

Quality control of herbal medicines

Liang

Xie²,

Chan

2004

Journal of Chromatography B

327

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Different chromatographic and electrophoretic techniques commonly used in the instrumental inspection of herbal medicines (HM) are first comprehensively reviewed. Chemical fingerprints obtained by chromatographic and electrophoretic techniques, especially by hyphenated chromatographies, are strongly recommended for the purpose of quality control of herbal medicines, since they might represent appropriately the "chemical integrities" of the herbal medicines and therefore be used for authentication and identification of the herbal products. Based on the conception of phytoequivalence, the chromatographic fingerprints of herbal medicines could be utilized for addressing the problem of quality control of herbal medicines. Several novel chemometric methods for evaluating the fingerprints of herbal products, such as the method based on information theory, similarity estimation, chemical pattern recognition, spectral correlative chromatogram (SCC), multivariate resolution, etc. are discussed in detail with examples, which showed that the combination of chromatographic fingerprints of herbal medicines and the chemometric evaluation might be a powerful tool for quality control of herbal products.

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Naturally Occurring Triterpenoid Saponins

Dinda

Debnath²,

Mohanta

et al. 2010

Chemistry & Biodiversity

140

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Naturally occurring new triterpenoid saponins reported from mid-1996 to March, 2007 are reviewed including their physical constants and plant sources, and are compiled in Table 1. New saponins are arranged in Table 1 on the basis of the skeletal structures of their aglycones, e.g., oleanane type, ursane type, lupane type, hopane type, taraxastane type, cycloartane type, lanostane type, tirucallane type, dammarane type, cucurbitane type, and holostane type. The known triterpenoid saponins and prosapogenins of the new saponins, the biological and pharmacological activities of which were published during 1996-2007, are also reviewed together with their plant sources listed in Table 2 according to the skeletal structures of their aglycones in the same fashion as in Table 1. The plant and animal sources of both new and known bioactive triterpenoid saponins are collected in Table 3 in alphabetical order. The biological and pharmacological activities such as antiallergic, antiatherosclerosis and antiplatelet, antibacterial, anticomplementary, antidiabetic, contraceptive, antifungal, anti-inflammatory, antileishmanial, antimalarial/antiplasmodial, anti-obesity, anti-proliferative, antipsoriatic, antispasmodic, antisweet, antiviral, cytotoxic/antitumor, detoxication, gastroprotective, haemolytic, hepatoprotective, immunomodulatory, anti-enzyme, anti-osteoporotic, insecticidal, insulin-like, membrane-porosity, molluscicidal, neuropharmacological, anti-endothelial dysfunction, snake venom antidote, and sweet activities of these saponins or derived prosapogenins are discussed briefly after Table 3.

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Liquid chromatographic method for analysis of saponins in Maesa balansae extract active against leishmaniasis

Cited by 10 publications

References 5 publications

Intravenous and Subcutaneous Toxicity and Absorption Kinetics in Mice and Dogs of the Antileishmanial Triterpene Saponin PX-6518

Intravenous and Subcutaneous Toxicity and Absorption Kinetics in Mice and Dogs of the Antileishmanial Triterpene Saponin PX-6518

Quality control of herbal medicines

Naturally Occurring Triterpenoid Saponins

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