Liquid chromatographic separation of analyte anions with iron(II) 1,10-phenanthroline as a mobile-phase additive:  origin and parameters in indirect detection

Rigas, Pantelis G.; Pietrzyk, Donald J.

doi:10.1021/ac00156a017

Cited by 31 publications

(7 citation statements)

References 38 publications

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“…In the separations reported here positive peaks were obtained because strong eluent counteranions, such as succinate or C104~, were used. For weaker eluent counteranions, such as CT or OAc", negative analyte peaks depending on the analyte were obtained in agreement with the origin of ID (13). System peaks (SP) due to the presence of counteranions and their contribution to the equilibria will also be detected.…”

Section: Methodssupporting

confidence: 60%

“…A fixed-volume 20-^L loop was used in combination with a Micromeritics 725 automatic injector or a Rheodyne 7125 injector. RESULTS AND DISCUSSION The Ru(II) complexes of 2,2'-bipyridine (bpy) and 1,10phenanthroline (phen) can be used as mobile phase II additives for the separation of inorganic and organic analyte anions like the corresponding Fe(II) complexes (11)(12)(13). The two key equilibria involved in the separation are (1) retention of the Ru(II) complex as a primary layer and its counteranion as a diffuse secondary layer onto the PRP-1 stationary phase and (2) competition between the counteranion and the analyte anion according to mass action and anion selectivity.…”

Section: Methodsmentioning

confidence: 99%

“…A third approach that is versatile in scope is to use a detector active ion interaction (II) reagent (ion pairing reagent) as a mobile phase additive. Most studies have focused on an additive and/or its counterion that is chromophoric (8)(9)(10)(11)(12)(13). In addition to its involvement in ID the II additive is also responsible for the separation (11,13).…”

mentioning

confidence: 99%

“…Most studies have focused on an additive and/or its counterion that is chromophoric (8)(9)(10)(11)(12)(13). In addition to its involvement in ID the II additive is also responsible for the separation (11,13).…”

mentioning

confidence: 99%

“…We have recently shown that iron(II) 1,10-phenanthioline salts are excellent II reagents for the separation and indirect photometric detection (IPD) of inorganic and organic analyte anions (11)(12)(13). The studies discussed here focus on the use of highly fluorescent ruthenium(II) 1,10-phenanthroline, Ru(phen)32+, and ruthenium(II) 2,2'-bipyridine, Ru(bpy)32+, salts as mobile phase II reagents for the separation and IFD of inorganic and organic analyte anions.…”

mentioning

confidence: 99%

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Ruthenium(II) complexes as ion interaction reagents for the liquid chromatographic separation and indirect fluorometric detection of analyte anions

Rigas¹,

Pietrzyk²

1988

Anal. Chem.

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Section: Methodssupporting

confidence: 60%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Ruthenium(II) complexes as ion interaction reagents for the liquid chromatographic separation and indirect fluorometric detection of analyte anions

Rigas¹,

Pietrzyk²

1988

Anal. Chem.

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Synthesis, Characterization, Spectroscopic, and Electrochemiluminescence Properties of a Solvatochromic Azacrown‐Containing Cyanoruthenate(II): Potential Applications in Separation and Indirect Photometric Detection of Cations and Amino Acids in HPLC

Chu

Yam

2006

Chemistry A European J

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A new anionic ruthenium(II) complex, (Et4N)[Ru(tpyA18C6)(CN)3] (tpyA18C6=N-[4'-(2,2':6',2''-terpyridyl)]-1,4,7,10,13-pentaoxa-16-azacyclohexadodecane), has been synthesized and characterized. The complex was found to show pronounced solvatochromic behavior and, when dissolved in solution, changed its color from purple to yellow when the solvent system was varied from pure acetonitrile to pure water. Its absorption and emission energies in various solvents showed a linear dependence of the Gutman's acceptor number. The characteristic photoluminescence and electrochemiluminescence (ECL) of the complex were also found to be progressively quenched as the proportion of water in a water/acetonitrile mixture increased. Large changes in the chemical shifts of the 1H NMR and 13C NMR signals of [Ru(tpyA18C6)(CN)3]- in different solvents were observed. The complex has also been demonstrated to serve as a mobile-phase additive in high-performance liquid chromatography for separation of metal cations and amino acids. Comparison studies with the crown-free analogue, (Et4N)[Ru(tpy)(CN)3] (tpy = 2,2':6',2''-terpyridine), showed that other than the ion-pair effect, the allosteric host-guest interaction provided by the presence of the pendant crown was essential to the separation performance of the complex. Indirect detection of nonabsorbing analytes has been achieved by monitoring the absorbance changes of the eluent at the metal-to-ligand charge-transfer (MLCT) absorption band maximum of the complex at 445 nm. The effects of pH, ionic strength, and polarity of the mobile phase as well as the complex concentration on the selectivity and resolution have also been studied.

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Potentiometric ion‐pair titrations of metal ions forming complexes with 1,10‐phenanthroline and its derivatives

Vytřas

Varmužová

1994

Electroanalysis

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Simple coated-wire electrodes. prepared using an ordinan aluminum wire conductor covered with the plasticized poly(viny1 chloride) membrane. were used in potentiometric titrations of metal ions forming complexes with 1.10-phenanthroline and its derivatives. The method is based on the precipitation of ion pairs formed by metal phenanthroline complexes and tetraphenylborate. Titrations were accompanied with titration cun-es yielding v e n sharp potential breaks, so that ver). reproducible results could be taken even in titrations. of quite diluted solutions (down to 2.5 x 10-mol of the metal ion in the 25 cm3 of the sample titrated). Using the procedure described, the following cations were titratable in forms of their 1,lO-phenanthroline complexes: Zn". CdLi. Mn'-. Fez-, Fe'-, CoL-, Ni", Cu", VO"' , Ga3-. In'-. and TI'-. Some of the metal ion solutions, prepared from the salts of anal!.tical-reagent-gmde purin, can be recommended for use in standardization procedures of the sodium tetraphenylborate titrant. 2,9-Dimethyl-l ,lo-phenanthroline complexes of ZnL+, Cd'+. and Cu+ were also titrable. but the results were significantly erroneous. Metal 4,7-diphenyl-l,l 0-phenanthroline complexes were insoluble in water as well as in water/ethanol mixtures.

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Liquid chromatographic separation of analyte anions with iron(II) 1,10-phenanthroline as a mobile-phase additive: origin and parameters in indirect detection

Abstract: Analyte anions separated on a Hamilton PRP-1 reverse-phase column using Iron(II) 1,10-phenanthroKne [Fe(phen)32+] salts

Cited by 31 publications

References 38 publications

Ruthenium(II) complexes as ion interaction reagents for the liquid chromatographic separation and indirect fluorometric detection of analyte anions

Ruthenium(II) complexes as ion interaction reagents for the liquid chromatographic separation and indirect fluorometric detection of analyte anions

Synthesis, Characterization, Spectroscopic, and Electrochemiluminescence Properties of a Solvatochromic Azacrown‐Containing Cyanoruthenate(II): Potential Applications in Separation and Indirect Photometric Detection of Cations and Amino Acids in HPLC

Potentiometric ion‐pair titrations of metal ions forming complexes with 1,10‐phenanthroline and its derivatives

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