Liquid chromatographic tandem mass spectrometric validated method for pharmacokinetic estimation of flecainide in human plasma

Goswami, Dipanjan; Kumar, Ajay; Khuroo, Arshad; Monif, Tausif; Gurule, Sanjay; Thudi, Nageshwar Rao; Shrivastav, Vikesh Kumar; Dubey, Sunil Kumar

doi:10.1080/10601330902919797

Cited by 1 publication

(1 citation statement)

References 17 publications

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“…The plasma was collected from healthy, non-smoking volunteers who provided informed consent for use for research purposes. Blood samples were collected in K2 EDTA vacutainers from the volunteers [11].…”

Section: Blank Plasmamentioning

confidence: 99%

Development and Validation of an Lc-MS/MS Method for the Determination of Tenofovir and Emtricitabine

PRIYADARSHINI,

DEVI D.

2024

Int J App Pharm

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Objective: Therapeutic drug monitoring of tenofovir and emtricitabine, two commonly used antiretroviral drugs, is important to maximize effectiveness while minimizing side effects. Materials: A Liquid Chromatography-Mass spectrometry (LC-MS/MS) method was developed to quantify tenofovir and emtricitabine in human plasma samples. The method involves a simple solid phase extraction procedure followed by liquid chromatography separation using a Penta Fluoro Phenyl (PFP) column with a Phenomenex C18 column and a mobile phase of ammonium formate, acetonitrile, and methanol, achieving separation in under 4 min. Results: The method showed good accuracy, low limits of quantification, adequate recovery, minimal matrix effects, and specificity. Analyte stability under multiple storage conditions was demonstrated. Conclusion: The validated LC-MS/MS method provides a reliable tool for therapeutic drug monitoring and pharmacokinetic studies of anti-Human Immuno-deficiency Virus (HIV) regimens. The assay can be applied to large populations, especially in resource-poor settings, to help individualize dosing and improve clinical outcomes while reducing toxicity.

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Section: Blank Plasmamentioning

confidence: 99%