Liquid chromatography and capillary electrophoresis in glycomic and glycoproteomic analysis

Abstract

Glycosylation is one of the most significant and abundant post-translational modifications in cells. Glycomic and glycoproteomic analyses involve the characterization of oligosaccharides (glycans) conjugated to proteins. Glycomic and glycoproteomic analysis is highly challenging because of the large diversity of structures, low abundance, site-specific heterogeneity, and poor ionization efficiency of glycans and glycopeptides in mass spectrometry (MS). MS is a key tool for characterization of glycans and glycopeptides. However, MS alone does not always provide full structural and quantitative information for many reasons, and thus MS is combined with some separation technique. This review focuses on the role of separation techniques used in glycomic and glycoproteomic analyses, liquid chromatography and capillary electrophoresis. The most important separation conditions and results are presented and discussed. Graphical abstract

Research of saccharides and related biocomplexes: A review with recent techniques and applications

“…When saccharides are not separated with HILIC, for example, isomers [176], RP-LC and derivatization with PMP may solve the problems of separation performance, sensitivity, and repeatability for monosaccharides [179]. Furthermore, monolithic nano-LC and HILIC coupled with MS give efficiency in glycaemic and glycoproteic studies [181], and accuracy in the identification of structural functionalities. However, drawbacks appear due to sample preparation, since low-molecular saccharides may not be origin from poly-or oligosaccharides when their fragments are detected as monosaccharides [177,179].…”

Recent developments and applications of capillary and microchip electrophoresis in proteomics and peptidomics (mid‐2018–2022)

“…CZE is especially useful for the characterization of PTMs that influence the charge of peptides or proteins. Characterization of glycosylation of proteins in their intact form by different CE and LC methods has been recently reviewed [138,139].…”

A novel ultrasensitive chemiluminescence enzyme immunoassay by employment of a signal enhancement of horseradish peroxidase-luminol-hydrogen peroxide reaction for the quantitation of atezolizumab, a monoclonal antibody used for cancer immunotherapy

“…17 The available techniques for quantifying ATZ in plasma are currently limited. These techniques include liquid chromatography-tandem mass spectrometry (LC-MS/MS) [18][19][20] and enzyme-linked immunosorbent assays (ELISAs). 21,22 However, LC-MS/MS has several signicant drawbacks.…”