Liquid chromatography coupled with mass spectrometry in the analysis of alkaloids

Verpoorte, Robert; Niessen, W.M.A.

doi:10.1002/pca.2800050502

Cited by 32 publications

(14 citation statements)

References 69 publications

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“…Peak tailing showed to be reduced Phytochem Rev (2007) 6:207-234 229 for basic compounds as compared to traditional C 18 alkyl packing (O'Gara et al 1999). The choice of different MS interfaces for the analysis of alkaloids has been reviewed (Verpoorte and Niessen 1994). The use of LC-TSP-MS allowed quantification of tryptamine, ajmalicine, serpentine, catharanthine and tabersonine (Auriola et al 1989).…”

Section: Hplc Methods and Equipmentsmentioning

confidence: 98%

Analysis of Catharanthus roseus alkaloids by HPLC

Hisiger

Jolicœur

2007

Phytochem Rev

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Catharanthus roseus is a medicinal plant from which secondary metabolites used in chemotherapy to treat diverse cancers are extracted. The well known high value metabolites vincristine and vinblastine are just 2 of 130 alkaloids that can be found in C. roseus. However, only few (~11) of this high number of chemical entities are frequently analyzed and even fewer (~8) are available commercially. For more than 30 years, different analytical techniques have been developed to isolate and identify C. roseus metabolites, and then allowing revealing the therapeutic potential of C. roseus metabolites. Among few approaches, high performance liquid chromatography (HPLC) technique is still widely used for the separation and analysis of secondary metabolites such as those from C. roseus. This article thus reviews the most recent developments in HPLC analysis of alkaloids from C. roseus. Diverse considerations that are crucial to the efficiency of secondary metabolites separation and identification steps, such as biomass manipulation, extraction phase and protocols, HPLC separation and analysis protocols are reviewed in details.Examples of spectra obtained using the most common detectors are also shown and suggestions are made on how to proceed in developing efficient separation and identification methods at the analytical and semi-preparative scales.

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Section: Hplc Methods and Equipmentsmentioning

confidence: 98%

Analysis of Catharanthus roseus alkaloids by HPLC

Hisiger

Jolicœur

2007

Phytochem Rev

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“…Because of their therapeutical value, there is a need to develop rapid, sensitive, and accurate analytical methods for the analysis of these alkaloids, both in pharmaceutical preparations and in plant extracts. Several chromatographic methods, including thin layer chromatography [2], gas chromatography (GC) [3,4] and high performance liquid chromatography (HPLC) [5,6], as well as combined techniques such as GC-mass spectrometry (GC-MS) [7] and HPLC-MS [8] have been developed for the analysis of tropane alkaloids. Due to its high efficiency, flexibility, accuracy, and very high resolution, capillary electrophoresis (CE) has revealed an enormous separation potential for the analysis of plant secondary metabolites [9].…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis-diode array detection — electrospray mass spectrometry for the analysis of selected tropane alkaloids in plant extracts

et al. 1999

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Capillary zone electrophoresis, coupled to UV and interfaced with electrospray ionization mass spectrometry (ESI‐MS), is described for the simultaneous analysis of hyoscyamine and scopolamine. On‐line UV detection occurred at 22 cm from the inlet of the capillary and ESI‐MS monitoring was performed along the entire length of the capillary (85 cm). An alkaline solution of 40 mM ammonium acetate at pH 8.5 was suitable for the analysis of the alkaloids under consideration. Under the optimized conditions, including CE and ESI‐MS parameters, the two alkaloids were resolved within a short time and with very high sensitivity. The differentiation of hyoscyamine and its positional isomer littorine, commonly encountered in plant material, is also presented using up‐front collision‐induced dissociation. Finally, the developed method was applied to the analysis of these alkaloids in Belladonna leaf extract and in Datura candida x D. aurea hairy root extract.

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“…This, it is hoped, will give as broad a coverage as possible for phytochemical analyses by thermospray. The analysis of alkaloids, although an important field of application, will not be dealt with here because this is already the subject of a further specialist review (Verpoorte and Niessen, 1994). The use of TSP LC-MS in phytochemical analysis will be discussed here from different viewpoints: (i) as a universal LC detection technique (for detection of non-UV active compounds); (ii) as a specific detection tool (for trace analysis and quantitative analysis); (iii) as a method for the rapid on-line identification of metabolites (molecular weight and structural information) and; (iv) as an efficient complementary technique to other coupled (hyphenated) techniques such as HPLC-UV.…”

Section: Introductionmentioning

confidence: 99%

Thermospray liquid chromatography‐mass spectrometry in phytochemical analysis

Wolfender

Maillard

Hostettmann

1994

Phytochemical Analysis

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Liquid chromatography‐mass spectrometry (LC‐MS) has become a successful analytical tool and is no longer regarded as a pure research instrument without any routine practical applications. The development of the thermospray interface (TSP) has been of great help in this respect, allowing the direct use of standard HPLC conditions (1 mL/min, aqueous solvents) for the LC‐MS coupling. TSP LC‐MS has been used in phytochemical analysis as a powerful detection technique for on‐line identification and for quantification of plant constituents, even in trace amounts. Applications of this technique to different classes of plant metabolites such as polyphenols, terpenes, sugars, glucosinolates, alkaloids and glycosides are described. The potential of TSP LC‐MS in the analysis of crude plant extracts is discussed.

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Liquid chromatography coupled with mass spectrometry in the analysis of alkaloids

Cited by 32 publications

References 69 publications

Analysis of Catharanthus roseus alkaloids by HPLC

Analysis of Catharanthus roseus alkaloids by HPLC

Capillary electrophoresis-diode array detection — electrospray mass spectrometry for the analysis of selected tropane alkaloids in plant extracts

Thermospray liquid chromatography‐mass spectrometry in phytochemical analysis

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