Liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) based bioavailability determination of the major classes of phytochemicals

Stylos, Evgenios Κ.; Chatziathanasiadou, Μaria V.; Syriopoulou, Aggeliki; Tzakos, Andreas G.

doi:10.1016/j.jchromb.2016.12.022

Cited by 20 publications

(14 citation statements)

References 83 publications

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“…The phytochemical composition analysis allowed for the identification of saponins and tannins, phytochemical compounds with multiple biological properties (Diaz Carrasco et al, 2016;Stylos, Chatziathanasiadou, Syriopoulou, & Tzakos, 2017), in all the plants extracts studied (Table 1) A similar result was reported for the aqueous fractions of P. biglobosa leaf, stem bark and root methanolic extracts. However, when apolar solvents were used, only alkaloids were obtained from the chloroform fraction (Udobi & Onaolapo, 2009).…”

Section: Discussionsupporting

confidence: 73%

<b>Phytochemistry and antibacterial activity of aqueous and hydroalcoholic extracts of three medicinal plants against food pathogens

Santos

Silva

et al. 2018

Acta Sci. Biol. Sci.

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This study aimed to characterize the phytochemical compositions of three medicinal Brazilian plants' leaves and bast extracts, and to determine their antibacterial activity on three foodborne and waterborne bacterial pathogens. Parkia platycephala, Pouteria ramiflora and Lophanthera lactescens leaves and basts were collected and aqueous and hydroalcoholic extracts were prepared. Qualitative screening of the phytochemical extracts was performed with three replicates and in triplicate in order to identify the bioactive compounds. The Minimal Inhibitory Concentration and Minimal Bactericide Concentration were determined by microdilution in broth and Escherichia coli, Salmonella typhimurium and Staphylococcus aureus growth was observed on agar plates. Phytochemical composition analysis allowed for the identification of anthraquinones, catechins, saponins, tannins, sesquiterpenlactones and other lactones in the three plants' leaves and bast aqueous and hydroalcoholic extracts. Eighty-three percent of the plant extracts showed antibacterial activity against S. aureus, and P. platycephala extracts were the only ones that inhibited E. coli and S. typhimurium growth. The present study contributes significantly to the phytochemical composition characterization of three plant species commonly used in Brazilian traditional medicine. The plant extracts' in vitro antibacterial activity was demonstrated and catechins present in the extracts are, most likely, the bioactive compounds responsible for this action.

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Section: Discussionsupporting

confidence: 73%

<b>Phytochemistry and antibacterial activity of aqueous and hydroalcoholic extracts of three medicinal plants against food pathogens

Santos

Silva

et al. 2018

Acta Sci. Biol. Sci.

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“…In our daily diet, we consume a significant amount of these phytochemicals. They are readily digested and eliminated by our body, resulting in a short-lived pharmacologic window [ 221 ]. Researchers are striving to develop new approaches that will increase the stability of dietary phytochemicals.…”

Section: Limitation Safety Consideration and Future Prospects Ofmentioning

confidence: 99%

An Update on the Role of Dietary Phytochemicals in Human Skin Cancer: New Insights into Molecular Mechanisms

et al. 2020

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Human skin is continuously subjected to environmental stresses, as well as extrinsic and intrinsic noxious agents. Although skin adopts various molecular mechanisms to maintain homeostasis, excessive and repeated stresses can overwhelm these systems, leading to serious cutaneous damage, including both melanoma and non-melanoma skin cancers. Phytochemicals present in the diet possess the desirable effects of protecting the skin from damaging free radicals as well as other benefits. Dietary phytochemicals appear to be effective in preventing skin cancer and are inexpensive, widely available, and well tolerated. Multiple in vitro and in vivo studies have demonstrated the significant anti-inflammatory, antioxidant, and anti-angiogenic characteristics of dietary phytochemicals against skin malignancy. Moreover, dietary phytochemicals affect multiple important cellular processes including cell cycle, angiogenesis, and metastasis to control skin cancer progression. Herein, we discuss the advantages of key dietary phytochemicals in whole fruits and vegetables, their bioavailability, and underlying molecular mechanisms for preventing skin cancer. Current challenges and future prospects for research are also reviewed. To date, most of the chemoprevention investigations have been conducted preclinically, and additional clinical trials are required to conform and validate the preclinical results in humans.

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“…The first and foremost challenge is the problem of bioavailability [ 119 , 120 , 121 ]. Since a majority of these phytochemicals are part of normal human diet, they are efficiently metabolized and cleared by body.…”

Section: Challenges For Phytochemicals In Cancer Therapy and Emergmentioning

confidence: 99%

“…Since a majority of these phytochemicals are part of normal human diet, they are efficiently metabolized and cleared by body. They do not persist in physiological systems and the therapeutic effects are usually short-lived [ 119 ]. The other aspect of using phytochemicals in cancer therapy is the lack of target specificity.…”

Section: Challenges For Phytochemicals In Cancer Therapy and Emergmentioning

confidence: 99%

Cancer Chemoprevention by Phytochemicals: Nature’s Healing Touch

et al. 2017

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Phytochemicals are an important part of traditional medicine and have been investigated in detail for possible inclusion in modern medicine as well. These compounds often serve as the backbone for the synthesis of novel therapeutic agents. For many years, phytochemicals have demonstrated encouraging activity against various human cancer models in pre-clinical assays. Here, we discuss select phytochemicals—curcumin, epigallocatechin-3-gallate (EGCG), resveratrol, plumbagin and honokiol—in the context of their reported effects on the processes of inflammation and oxidative stress, which play a key role in tumorigenesis. We also discuss the emerging evidence on modulation of tumor microenvironment by these phytochemicals which can possibly define their cancer-specific action. Finally, we provide recent updates on how low bioavailability, a major concern with phytochemicals, is being circumvented and the general efficacy being improved, by synthesis of novel chemical analogs and nanoformulations.

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Liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) based bioavailability determination of the major classes of phytochemicals

Cited by 20 publications

References 83 publications

<b>Phytochemistry and antibacterial activity of aqueous and hydroalcoholic extracts of three medicinal plants against food pathogens

<b>Phytochemistry and antibacterial activity of aqueous and hydroalcoholic extracts of three medicinal plants against food pathogens

An Update on the Role of Dietary Phytochemicals in Human Skin Cancer: New Insights into Molecular Mechanisms

Cancer Chemoprevention by Phytochemicals: Nature’s Healing Touch

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