Liquid Chromatography–electrospray Tandem Mass Spectrometry (Lc-MS/Ms) Determination of Lansoprazole in Human Plasma

Abstract

& A rapid, selective, and sensitive LC-MS=MS method has been developed and validated for quantification of lansoprazole (LZ) in human plasma using esomeprazole as an internal standard (IS). The analyte and IS were extracted by single solvent liquid-liquid extraction using tert-Butyl methyl ether and separated by isocratic elution on C18 analytical column with 90:10 v=v acetonitrile, and 20 mM ammonium acetate (flow rate of 1 mL=min) as the mobile phase in the positive ion mode. Selected Reaction Monitoring transitions for LZ, internal standard and their daughter ion were observed at 369, 346, 252, and 198 m=z, respectively. The lower limit of quantification was 3.997 ng=mL and total run time for the analysis was 1.2 minutes. Linear calibration was observed in the range of 3.997 ng=mL to 2002.5 ng=mL. The method was validated for its selectivity, stability, accuracy, precision, and recovery.

Validated Simultaneous Gradient Ultra-Performance Liquid Chromatographic Quantification of Some Proton Pump Inhibitor Drug Residues in Saudi Pharmaceutical Industrial Wastewater

“…Additionally, actual wastewater samples were carefully treated using the optimized sample preparation technique, then they were chromatographed by applying the optimized chromatographic parameters ( Figure 2 ). The results were compared to those obtained by analyzing the actual wastewater samples using reference methods for the determination of OMP, PNT, and LNZ [ 9 , 17 , 25 ] after sample pretreatment by the same sample preparation protocol ( Table 4 ). Method performance for the determination of the cited drugs was also validated by the application of the standard addition procedure.…”

“…Additionally, the same technique was demonstrated for the simultaneous quantification of the studied drug with naproxen [ 24 ]. LC-MS/MS was applied for the determination of LNZ in human plasma, using esomeprazole as an IS [ 25 ].…”