Liquid chromatography/electrospray tandem mass spectrometry method for the determination of cefuroxime in human plasma: Application to a pharmacokinetic study

Partani, Pankaj; Gurule, Sanjay; Khuroo, Arshad; Monif, Tausif; Bhardwaj, Sandeep

doi:10.1016/j.jchromb.2009.12.025

Cited by 35 publications

(13 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…With an amino group and a carboxy group in its chemical structure, cefuroxime could be ionized under both positive and negative electrospray ionization conditions. However, the response was much higher in the negative mode compared with that in the positive mode (Partani et al ., ). From a full‐scan mass spectrum via the first quadrupole (Q1) mass filter, the [M − H] − at m/z 423.2 was found by introducing the [M − H] − ions into the second quadrupole (Q2) cell and, being dissociated via the Q3 mass filter, the product spectrum of the [M − H] − ion with a low CE of −20 eV gave a considerably better response and a higher signal, when a major fragment ion at m/z 206.8 was formed.…”

Section: Resultsmentioning

confidence: 97%

“…A wide variety of analytical techniques have been applied for the determination of cefuroxime in various biological fluids, including plasma, serum and urine using high-performance liquid chromatography with UV, single mass or tandem mass spectrometry (Carlier et al, 2012;Partani et al, 2010;Tuerk et al, 2006;Viberg et al, 2004). These methods require large volumes of biological samples, long run times and time-consuming cleanup procedures.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of cefuroxime lysine in rat brain microdialysates by ultra‐fast liquid chromatography with UV and tandem mass spectrometry: application to an acute toxicokinetic study

Zhao

Zhu

et al. 2014

Biomedical Chromatography

View full text Add to dashboard Cite

Cefuroxime lysine is a new second-generation cephalosporins, which can penetrate the blood-brain barrier to cure the meningitis. In order to investigate its acute toxicokinetic study after intraperitoneal injection of 675 mg/kg cefuroxime lysine, a sensitive and clean ultra-fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) method for the determination of cefuroxime lysine in microdialysate samples was developed and validated, which was compared with UFLC-UV as a reference method. Chromatographic separation was performed on a Shim-pack XR-ODS C18 column (75 × 3.0 mm, 2.2 µm), with an isocratic elution of 0.1% formic acid in acetonitrile-0.1% formic acid in water (45:55, v/v) for LC-MS and acetonitrile-20 mm potassium dihydrogen phosphate (pH 3.0,20:80, v/v) for LC-UV. The lower limit of detection was 0.01 µg/mL for LC-MS and 0.1 µg/mL for LC-UV method, with the same corresponding linearity range of 0.1-50 µg/mL. The intra- and inter-day precisions (relative standard deviation) for both methods were from 1.1 to 8.9%, while the accuracy was all within ±10.9%. The results of both methods were finally compared using paired t-test; the results indicated that the concentrations measured by the two methods correlated significantly (p < 0.05), which suggested that the two methods based on LC-MS and LC-UV were suitable for the acute toxicokinetic study.

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Determination of cefuroxime lysine in rat brain microdialysates by ultra‐fast liquid chromatography with UV and tandem mass spectrometry: application to an acute toxicokinetic study

Zhao

Zhu

et al. 2014

Biomedical Chromatography

View full text Add to dashboard Cite

show abstract

“…The pharmacokinetic properties of cefuroxime sodium have been extensively studied with the most appropriate pharmacokinetic model of a 2-compartment model [ 18 ]. Here, a 3-compartment model was selected in calculating the population pharmacokinetic, the possible reasons of that might be due to the difference in administration method (intravenous infusion) and/or the better accuracy in the chemical analysis.…”

Section: Discussionmentioning

confidence: 99%

Bioequivalence and Population Pharmacokinetic Modeling of Two Forms of Antibiotic, Cefuroxime Lysine and Cefuroxime Sodium, after Intravenous Infusion in Beagle Dogs

Zhao

et al. 2012

Journal of Biomedicine and Biotechnology

View full text Add to dashboard Cite

To investigate the bioequivalence and the population pharmacokinetics of cefuroxime lysine and cefuroxime sodium in healthy beagle dogs. A randomized 2-period crossover design in 18 healthy beagle dogs after receiving 20, 40, and 80 mg/kg of cefuroxime lysine or cefuroxime sodium was conducted. A 3-compartment open model was used as the basic model for the population pharmacokinetic study. Both of the antibiotics exhibited dose-proportional pharmacokinetics over the dose range of 20–80 mg/kg. The mean relative bioavailability of cefuroxime lysine versus cefuroxime sodium was 1.05 (range, 0.71 to 1.42), with a significant difference between males and females. The estimates of population pharmacokinetic of CL, V1, Q2, V2, Q3, V3 were 3.74 mL/h, 1.70 mL, 29.5 mL/min, 3.58 mL, 0.31 mL/min, and 158 mL for cefuroxime lysine and 4.10 mL/h, 1.00 mL, 38.5 mL/min, 4.19 mL, 0.06 mL/min, and 13.6 mL for cefuroxime sodium, respectively. The inter-individual variability was determined to be less than 29.1%. A linear pharmacokinetic was revealed for cefuroxime lysine and cefuroxime sodium in dogs after intravenous infusion, and the bioequivalence of these forms of the antibiotic was observed with the significant gender-related differences in mean relative bioavailability of cefuroxime lysine versus cefuroxime sodium.

show abstract

“…The cephalosporins are a subclass of the ␤-lactam antibiotics, and include drugs such as cephalexin, cefixime, cefuroxime and cefoperazone. C18 is the most popular analytical column used for the cephalosporins, and they contain both amino and carboxylic acid groups, so can ionise in both negative and positive mode [163][164][165][166][167].…”

Section: Antibioticsmentioning

confidence: 99%

Therapeutic drug monitoring and LC–MS/MS

Adaway

Keevil

2012

Journal of Chromatography B

130

View full text Add to dashboard Cite

Liquid chromatography/electrospray tandem mass spectrometry method for the determination of cefuroxime in human plasma: Application to a pharmacokinetic study

Cited by 35 publications

References 20 publications

Determination of cefuroxime lysine in rat brain microdialysates by ultra‐fast liquid chromatography with UV and tandem mass spectrometry: application to an acute toxicokinetic study

Determination of cefuroxime lysine in rat brain microdialysates by ultra‐fast liquid chromatography with UV and tandem mass spectrometry: application to an acute toxicokinetic study

Bioequivalence and Population Pharmacokinetic Modeling of Two Forms of Antibiotic, Cefuroxime Lysine and Cefuroxime Sodium, after Intravenous Infusion in Beagle Dogs

Therapeutic drug monitoring and LC–MS/MS

Contact Info

Product

Resources

About