Liquid Chromatography in Pharmaceutical Analysis V: Determination of an Isoniazid–Pyridoxine Hydrochloride Mixture

Stewart, James T.; Honigberg, I. L.; Brant, J.P.; Murray, W. A.; Webb, Julie L.; Smith, Jessalyn

doi:10.1002/jps.2600651032

Cited by 24 publications

(5 citation statements)

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“…This has prompted many investigators to plan methods for the rapid determination of INH in its pure form as well as in pharmaceutical preparations. There are various analytical procedures for the assay of INH, the most important being titrimetry [9,10], visible spectrophotometry [11][12][13], polarography [14], coulometry [15], high-performance liquid chromatography [16], and fluorimetry [17] methods. The spectrophotometric methods involve the use of reagents such as chloranil [18], 4-nitrobenzaldehyde, pyridoxal [19], 4-dimethylaminobenzaldehyde [20], and so forth.…”

Section: Introductionmentioning

confidence: 99%

Novel Reagents for the Spectrophotometric Determination of Isoniazid

2012

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Isoniazid is an antitubercular drug, widely used for tuberculosis. Owing to its importance in therapeutics, the present study aims to develop simple method for the spectrophotometric determination of isoniazid (INH). Two novel reagents, epichlorohydrine (ECH) and 4-hydroxyphenaylchloride (HPC) are used for the spectrophotometric determination of INH. Based on the nucleophilic substitution reactions of INH with EPI & HPC in basic medium, rapid, simple, inexpensive, precise, and accurate visible spectrophotometric method is proposed for the determination of INH in bulk drug and in formulations. Method involves the reaction of INH with EPI and HPC in basic medium to form yellow-colored chromogen, measuring the absorbances at 405 and 402 nm for INH-EPI & INH-HPC, respectively. The optimum experimental conditions have been studied. The absorbance was found to increase linearly with the concentration of the drug and formed the basis for quantification. The calibration graphs were linear from 2.00–22.00 μg mL−1and 20.00–120.00 μg mL−1for INH-EPI & INH-HPC, respectively. The apparent molar absorptivity and Sandell's sensitivity are calculated to be0.51×104&0.10×104 L mol−1 cm−1and 0.027 & 0.134 μg cm−2for INH-EPI & INH-HPC, respectively. The procedure is used to determine INH in pharmaceutical products. The associated pharmaceutical materials do not interfere in the measurements.

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Section: Introductionmentioning

confidence: 99%

Novel Reagents for the Spectrophotometric Determination of Isoniazid

2012

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“…The noncharged solutes such as meprobamate, carisoprodol, and methocarbamol had a wide range of retention times in methanolwater systems (k' = 0. [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. Increasing the polarity of the mobile phase (Solvents A -C) predictably increased the k' value for any selected neutral solute.…”

Section: Results a N D Discussionmentioning

confidence: 99%

“…A prodrug (11) that regenerates acronine quantitatively and shows improved water solubility was prepared but suffers from the disadvantage of being hydrolyzed too rapidly under physiological conditions (3). Reaction of I1 with aniline gave a rapid quantitative yield of ani12, and the hydrolysis rates were similar for a series of analogs of 11 in which the acetyl group was replaced by other acyl functions (2).…”

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confidence: 91%

“…Various attempts have been made (2) to improve the formulation of I, including the preparation of molecular complexes of the alkaloid with povidone and gentisic acid. A prodrug (11) that regenerates acronine quantitatively and shows improved water solubility was prepared but suffers from the disadvantage of being hydrolyzed too rapidly under physiological conditions (3).…”

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confidence: 99%

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Liquid Chromatography in Pharmaceutical Analysis XI: Determination of Muscle Relaxant-Analgesic Mixtures Using Reversed-Phase and Ion-Pair Techniques

Stewart

Honigberg

Coldren

1979

Journal of Pharmaceutical Sciences

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“…The determination of isoniazid in pharmaceutical preparation and biological fluids has appeared especially attractive. For its measurement, many methods have been developed, such as liquid chromatography (2,3), titrimetry (4), spectrophotometry (5,6), fluorimetry (7), electrochemical detection (8,9), and capillary electrophoresis (10). These methods often suffer from a variety of disadvantages, such as they could be sophisticated, time-consuming, or require expensive instrument.…”

Section: Introductionmentioning

confidence: 99%