Liquid chromatography–mass spectrometry-based quantitative proteomics analysis reveals chondroprotective effects of astragaloside IV in interleukin-1β-induced SW1353 chondrocyte-like cells

Luo, Huali; Yao, Ling; Zhang, Yudi; Li, Rongheng

doi:10.1016/j.biopha.2017.04.127

Cited by 23 publications

(10 citation statements)

References 26 publications

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“…The current study also revealed that DEGs enriched in positive regulation of cell migration and negative regulation of cell proliferation. Meanwhile, ECM-receptor interaction was found to be the most significantly enriched pathway for DEGs, which was further verified by many previous studies [36, 37]. Therefore, dysfunction of these cell phenotypes and molecules may play important roles in OA development and can act as promising pathological signatures for OA in vitro and in vivo.…”

Section: Discussionsupporting

confidence: 70%

Transcriptome analyses identify key genes and potential mechanisms in a rat model of osteoarthritis

2018

J Orthop Surg Res

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BackgroundOsteoarthritis (OA) is one of the most common degenerative diseases of the joints worldwide, but still the pathogenesis of OA is largely unknown. The purpose of our study is to clarify key candidate genes and relevant signaling pathways in a surgical-induced OA rat model.MethodsThe microarray raw data of GSE8077 was downloaded from GEO datasets. GeoDiver were employed to screen differentially-expressed genes (DEGs). Enrichment analyses of DEGs were performed using Metascape. Construction of protein–protein interaction (PPI) network and identification of key genes were conducted using STRING, Cytoscape v3.6.0, and Centiscape2.2. Furthermore, miRDB and Cytoscape v3.6.0 were used for visualization of miRNA-mRNA regulatory network. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis for predicted miRNAs was undertaken using DIANA-miRPath v3.0.ResultsSeveral DEGs (188 in comparison between OA and sham-operated group and 160 in comparison between OA and contralateral group) were identified. DEGs mainly enriched in vasculature development, regulation of cell migration, response to growth factor (Gene ontology), and ECM-receptor interaction (KEGG). Two comparison cohorts shared 79 intersection genes, and of these, Ccl2, Col4a1, Col1a1, Aldh1a3, and Itga8 were defined as the hub genes. Predicted miRNAs of seven DEGs from sub-networks mainly enriched in MAPK signaling pathway.ConclusionThe current study shows that some key genes and pathways, such as Ccl2, Col4a1, Col1a1, Aldh1a3, Itga8, ECM-receptor interaction, and MAPK signaling pathway may be associated with OA progression and act as potential biomarkers and therapeutic targets for OA.Electronic supplementary materialThe online version of this article (10.1186/s13018-018-1019-3) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 70%

Transcriptome analyses identify key genes and potential mechanisms in a rat model of osteoarthritis

2018

J Orthop Surg Res

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“…However, there are a few studies showing that YAP1 plays a protective role in the progression of OA. These results of these studies suggest that YAP1 can delay the progression of OA and may allow for the treatment of OA by upregulating YAP1 (Luo et al, 2017;Deng et al, 2018;Fu et al, 2019). A recently published study by Deng et al (2018) showed that YAP1 activation by transgene overexpression or deletion of its upstream inhibitory kinase Mst1/2 can protect the integrity of articular cartilage, while the loss of YAP1 in chondrocytes may promote cartilage destruction.…”

Section: Yap1 Is An Important Promoter Of Oamentioning

confidence: 82%

Yes-Associated Protein 1: Role and Treatment Prospects in Orthopedic Degenerative Diseases

Xie

Xiao

Tang

et al. 2020

Front. Cell Dev. Biol.

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The Hippo/yes-associated protein 1 signaling pathway is an evolutionarily conserved signaling pathway. This signaling pathway is primarily involved in the regulation of stem cell self-renewal, organ size and tissue regeneration by regulating cell proliferation, differentiation and apoptosis. It plays an important role in embryonic development and tissue organ formation. Yes-associated protein 1 (YAP1) is a key transcription factor in the Hippo signaling pathway and is negatively regulated by this pathway. Changes in YAP1 expression levels affect the occurrence and development of a variety of tumors, but the specific mechanism associated with this phenomenon has not been thoroughly studied. Recently, several studies have described the role of YAP1 in osteoarthritis (OA). Indeed, YAP1 is involved in orthopedic degenerative diseases such as osteoporosis (OP) in addition to OA. In this review, we will summarize the significance of YAP1 in orthopedic degenerative diseases and discuss the potential of the targeted modulation of YAP1 for the treatment of these diseases.

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“…It has previously been reported that the chondrocyte ECM serves an important role in cartilage function (29), and that the dynamic equilibrium between matrix synthesis and degradation maintains the stability and integrity of cartilage tissue (30). A recent study has reported that the Hippo signaling pathway and ECM-receptor pathway are involved in the process of osteoarthritic chondrocyte apoptosis (31). In addition, the results of the present study revealed that the purple module was primarily enriched in response to extracellular stimulus, FoxO signaling pathway and human T-cell leukemia virus 1 infection.…”

Section: Discussionmentioning

confidence: 99%

Identification of key gene modules and transcription factors for human osteoarthritis by weighted gene co‑expression network analysis

Gao

Sun

2019

Exp Ther Med

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Osteoarthritis (OA) is one of the most prevalent causes of joint disease. However, the pathological mechanisms of OA have remained to be completely elucidated, and further investigation into the underlying mechanisms of OA development and the identification of novel therapeutic targets are urgently required. In the present study, the dataset GSE114007 was downloaded from the Gene Expression Omnibus database. Based on weighted gene co-expression network analysis (WGCNA) and the identification of differentially expressed genes (DEGs), the microarray data were further analyzed to identify hub genes, key transcription factors (TFs) and pivotal signaling pathways involved in the pathogenesis of OA. A total of 1,898 genes were identified to be differentially expressed between OA samples and normal samples. Based on WGCNA, the present study identified 5 hub modules closely associated with OA, and the potential key TFs for hub modules were further explored based on CisTargetX. The results demonstrated that B-Cell Lymphoma 6, Myelin Gene Expression Factor 2, Activating Transcription Factor 3, CCAAT Enhancer Binding Protein γ, Nuclear Factor Interleukin-3-Regulated, FOS Like Antigen-2, FOS-Like Antigen-1, Fos Proto-Oncogene, JunD Proto-Oncogene, Transcription Factor CP2 Like 1, RELA proto-oncogene NF-kB subunit, SRY-box transcription factor 3, V-Ets Avian Erythroblastosis Virus E26 Oncogene Homolog 2, Interferon Regulatory Factor 4 and REL proto-oncogene, NF-kB subunit were the potential key TFs. In addition, osteoclast differentiation, FoxO, MAPK and PI3K/Akt signaling pathways were revealed to be imperative for the pathogenesis of OA, as these 4 pivotal signaling pathways were observed to be tightly linked through 4 key TFs Fos Proto-Oncogene, JUN, JunD Proto-Oncogene and MYC, and 4 DEGs Vascular Endothelial Growth Factor A, Growth Arrest and DNA Damage Inducible α, Growth Arrest and DNA Damage Inducible β and Cyclin D1. The present study identified a set of potential key genes and signaling pathways, and provided an important opportunity to advance the current understanding of OA.

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Liquid chromatography–mass spectrometry-based quantitative proteomics analysis reveals chondroprotective effects of astragaloside IV in interleukin-1β-induced SW1353 chondrocyte-like cells

Cited by 23 publications

References 26 publications

Transcriptome analyses identify key genes and potential mechanisms in a rat model of osteoarthritis

Transcriptome analyses identify key genes and potential mechanisms in a rat model of osteoarthritis

Yes-Associated Protein 1: Role and Treatment Prospects in Orthopedic Degenerative Diseases

Identification of key gene modules and transcription factors for human osteoarthritis by weighted gene co‑expression network analysis

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