Liquid chromatography method for simultaneous quantification of ATP and its degradation products compatible with both UV–Vis and mass spectrometry

Law, Andrew; Hafen, Paul S.; Brault, Jeffrey J.

doi:10.1016/j.jchromb.2022.123351

Cited by 21 publications

(7 citation statements)

References 36 publications

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“…Inosine-hypoxanthine solution was measured using an HPLC-UV detector according to the protocol described by Law et al [15]. The prepared supernatant was analyzed using a Waters T3 column (5 um, 250 × 4.6 mm) connected to an HPLC pump (Alliance e2695; Waters Corp., Massachusetts, USA) and a Waters 2998 UV detector.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of purine-nucleoside degrading ability andin vivouric acid lowering ofStreptococcus thermophilus(ID-PDP3), a novel antiuricemia strain

Kim,

Moon,

Kim

et al. 2023

Preprint

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This study evaluated 15 lactic acid bacteria in terms of their ability to degrade inosine and hypoxanthine—which are the intermediates in purine metabolism—for the management of hyperuricemia and gout. After a preliminary screening based on HPLC, CR1 (Lactiplantibacillus plantarum) and GZ1 (Lactiplantibacillus pentosus) showed the highest nucleoside degrading rates and were therefore selected for further characterization. IDCC 2201 (S. thermophilus), which possessed thehptgene encoding hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and exhibited purine degradation, was also selected. These three selected strains were examined in terms of the effect of probiotics on lowering serum uric acid in a rat model of potassium oxonate (PO)-induced hyperuricemia. Among them, the level of serum uric acid was most reduced by IDCC 2201 (p < 0.05). Further, analysis of the microbiome showed that administration of IDCC 2201 recovered the ratio of Bacteroidetes/Firmicutes in the intestinal microbial composition unbalanced by hyperuricemia and showed a difference in the intestinal microbial composition compared to the group administered with allopurinol. Moreover, intestinal short-chain fatty acids (SCFAs) were significantly increased. Ultimately, the findings show that IDCC 2201 lowers uric acid levels by degrading purine-nucleosides and restores intestinal flora and SCFAs, ultimately suggesting that IDCC 2201 is a promising candidate for use as an adjuvant treatment in patients with hyperuricemia.

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Section: Methodsmentioning

confidence: 99%

“…Inosine-hypoxanthine solution was measured using an HPLC-UV detector according to the protocol described by Law et al [15]. The prepared supernatant was analyzed using a Waters T3 The quantification of each compound was performed based on the peak area value of the HPLC chart.…”

Section: Screening Of Labs Capable Of Degrading Inosine and Hypoxanthinementioning

confidence: 99%

Evaluation of purine-nucleoside degrading ability andin vivouric acid lowering ofStreptococcus thermophilus(ID-PDP3), a novel antiuricemia strain

Kim,

Moon,

Kim

et al. 2023

Preprint

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“…Currently, a variety of analytical methods have already been used to detect ATP, such as electrochemical methods, 7 high performance liquid chromatography, 8 colorimetric methods, 9 surface-enhanced Raman spectroscopy (SERS), 10,11 and fluorescence analysis methods. 12,13 Nevertheless, these techniques still have many shortcomings and limitations.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Currently, a variety of analytical methods have already been used to detect ATP, such as electrochemical methods, high performance liquid chromatography, colorimetric methods, surface-enhanced Raman spectroscopy (SERS), , and fluorescence analysis methods. , Nevertheless, these techniques still have many shortcomings and limitations. For example, the electrochemical method has the advantages of being fast and sensitive, but it requires complex modifications of electrodes and is time-consuming.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial-Targeted AIE-Active Fluorescent Probe Based on Tetraphenylethylene Fluorophore with Dual Positive Charge Recognition Sites for Monitoring ATP in Cells

et al. 2023

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Adenosine triphosphate (ATP), as an important intracellular energy currency produced in mitochondria, is closely related to various diseases in living organisms. Currently, the biological application of AIE fluorophore as a fluorescent probe for ATP detection in mitochondria is rarely reported. Herein, D−π−A and D−A structure-based tetraphenylethylene (TPE) fluorophores were employed to synthesize six different ATP probes (P1−P6), and the phenylboronic acid groups and dual positive charge sites of probes could interact with the vicinal diol of ribose and negatively charged triphosphate structure of ATP, respectively. However, P1 and P4 with a boronic acid group and a positive charge site had poor selectivity for ATP detection. In contrast, P2, P3, P5, and P6 with dual positive charge sites exhibited better selectivity than P1 and P4. In particular, P2 had more advantages of high sensitivity, selectivity, and good time stability for ATP detection than P3, P5, and P6, which was ascribed to its D−π−A structure, linker 1 (1,4-bis(bromomethyl)benzene), and dual positive charge recognition sites. Then, P2 was employed to detect ATP, and it exhibited a low detection limit of 3.62 μM. Moreover, P2 showed utility in the monitoring of mitochondrial ATP level fluctuations.

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“…There are some traditional methods available for ATP detection, such as high-performance liquid chromatography, − nuclear magnetic resonance spectroscopy, and capillary electrophoresis . However, most of these assays require expensive instruments and complicated operations.…”

Section: Introductionmentioning

confidence: 99%

Water-Soluble Conjugated Polyelectrolytes for Adenosine Triphosphate (ATP) Detection

Zhang

Duan

Hong

et al. 2023

ACS Appl. Polym. Mater.

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Facile detection of adenosine triphosphate (ATP) at different concentration levels is important for applications such as assessing physiological status and monitoring bacterial and microbial contamination of food. A series of cationic conjugated polymers, PFPE-TEG x -NMe3 +, with poly[fluorenyl-alt-p-phenyleneethynylene] (PFPE) as the backbone and different ratios of side chains consisting of a hexyl quaternary ammonium group and triethylene glycol, were synthesized for ATP sensing. Careful calculations based on the integrals of several specific proton peaks in the NMR spectra suggest that the ratios of different side chains of obtained polymers were consistent with the feeding ratios and the quaternization was close to 100%. Sensing application found that the fluorescence of polymers in aqueous solution was quenched upon adding ATP. The limit of detection (LOD) decreased with the increase in cation density on the polymer, which could be as low as 0.25 μM, and the higher the density of the quaternary ammonium group, the higher the sensitivity of polymer to ATP. The sensing system was selective to ATP over other possible interferents. Dynamic light scattering and zeta potential measurements show that ATP molecules were bound to polymer chains mainly through electrostatic interaction, while other interactions also existed, resulting in the formation of aggregates and the consequent fluorescence quenching. Relating the sensing performance to the chemical structures of analytes and fluorescent polymers revealed that the four negative charges combined with the organic part with a π-structure and some other interaction sites, which makes ATP unique compared with those possible interferents. At relatively low densities, the density of cationic groups had a significant effect on LOD and sensitivity, but at high densities, its effect became smaller; this observation is consistent with a sensing mechanism based on multiple interaction synergies dominated by electrostatic interactions. This study provides a general strategy for the design of sensing systems for biomolecules with relatively complex structures.

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Liquid chromatography method for simultaneous quantification of ATP and its degradation products compatible with both UV–Vis and mass spectrometry

Cited by 21 publications

References 36 publications

Evaluation of purine-nucleoside degrading ability andin vivouric acid lowering ofStreptococcus thermophilus(ID-PDP3), a novel antiuricemia strain

Evaluation of purine-nucleoside degrading ability andin vivouric acid lowering ofStreptococcus thermophilus(ID-PDP3), a novel antiuricemia strain

Mitochondrial-Targeted AIE-Active Fluorescent Probe Based on Tetraphenylethylene Fluorophore with Dual Positive Charge Recognition Sites for Monitoring ATP in Cells

Water-Soluble Conjugated Polyelectrolytes for Adenosine Triphosphate (ATP) Detection

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