Liquid Chromatography of Amino Acids and their Derivatives

Perrett, David

doi:10.1007/978-94-009-4832-7_15

Cited by 9 publications

(6 citation statements)

References 147 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comprising PEG 300, 1000, 1450, 2000 Separation of Amino Acids Mixtures by Reverse-Phase Chromatography Preliminary work established optimal separation conditions for amino acid quantitation using reverse-phase chromatography (RPC). The separation of glycine and threonine was not achieved despite the following reported RPC procedures (see Perrett, 1985), while tryptophan, methionine, valine, leucine, and phenylalanine, eluted with similar retention times. Methionine in ATPS samples yielded a characteristic two-peak chromatogram apparently in¯uenced by the presence of PEG.…”

Section: Biological Feedstocksmentioning

confidence: 97%

Characterization of aqueous two‐phase partition systems by distribution analysis of radiolabeled analytes (DARA): Application to process definition and control in biorecovery

Lebreton

Walker

Lyddiatt

2001

Biotech & Bioengineering

View full text Add to dashboard Cite

In this article, we describe a characterization method applicable to aqueous two-phase systems (ATPS) heavily loaded with complex biological feed-stocks. We also studied the partition behavior of mixtures of traceable and quantifiable radiolabeled amino acids, selected on the basis of their relative hydrophobicity A unique linear relation was established between the tie-line length (TLL: commonly determined by graphical methods) and the hydrophobic factor (HF) for ATPS comprising potassium phosphate and PEG alone, and validated for polymer molecular weights from 300 to 8000 Da in systems operated at an apparent pH value of 7.5. Radiolabeled amino acids were subsequently applied to the characterization of ATPS loaded with whole bovine blood by the determination of effective tie-line lengths (TLL(e)). The addition of biomass to ATPS increased TLL(e) relative to that of blank ATPS of equivalent original composition of PEG and phosphate. In addition, an increase of biomass loading (variously sourced from blood, yeast, and E. coli) contributed to phase formation and stabilization of loaded ATPS in respect of system sensitivity toward operational conditions. The controlled application of sensitive ATPS (adjacent to the binodal curve) could thus be reconsidered for further application of aqueous two-phase partitioning as a primary purification process. The application of effective tie-line determinations by distribution analysis of radiolabeled analytes (DARA) as a process-aid in the design and operation of ATPS in biorecovery is discussed.

show abstract

Section: Biological Feedstocksmentioning

confidence: 97%

Characterization of aqueous two‐phase partition systems by distribution analysis of radiolabeled analytes (DARA): Application to process definition and control in biorecovery

Lebreton

Walker

Lyddiatt

2001

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…More detailed overviews of HPLC (Bidlingmeyer 1992) with specific applications to amino acids and GC (Hare et ul. 1985;Perrett 1985;Gooding and Regnier 1990) can be found elsewhere.…”

Section: Methodsmentioning

confidence: 99%

“…In RPLC, the most polar compounds are eluted from the column first, followed by the least polar compounds. RPLC is used for separation and quantification of amino acids, peptides and proteins (Krstulovic and Brown 1982;Perrett 1985; Ahuja 199 1 ; Ostrom et d. 19941, and is not routinely used for geochronological purposes at this point.…”

Section: Reversed-phase Liquid Chromatographymentioning

confidence: 99%

Archaeological Applications of Amino Acid Racemization*

Johnson

Miller

1997

Archaeometry

View full text Add to dashboard Cite

Amino acid racemization (AAR) in fossil biominerals has been used over the last 30 years to develop reliable chronologies and thermal histories in archaeological settings from all over the world. It is a biochemical reaction which progresses with time and temperature, and has the potential to be used in any material capable of sequestering amino acids (e.g., avian eggshell, molluscs, teeth, and bones). AAR is not a numerical dating method, per se; however, it can be used for a variety of chronological and palaeotemperature applications. Provided there is some independent age control, AAR can be used to extend or to improve upon the chronology, or to reconstruct the temperature history at a site. The use of AAR as a dating/palaeothermometry tool has been somewhat underutilized by the archaeological community, presumably due to a series of errors made on studies of human bone shortly after the technique was first discovered. Since these early studies, continued exploration of AAR has demonstrated the growing power of the technique for chronological and palaeothermometry research in certain sample types, as well as some novel applications pertinent to archaeologists and anthropologists. For example, relevant information on human behaviour, including burial practices, the use of fire, and the degree of mixing at a site, can be determined using AAR. This paper presents the basic principles behind AAR and a review of the technique as it has been applied to the archaeological record. AAR in some of the biominerals commonly associated with archaeological sites is reviewed, and guidelines for sample collection presented. Finally, a brief description of some of the chromatography techniques used for amino acid analysis is given.

show abstract

“…There have been several chromatographic methods reported which aim at the chiral separation of L-and D-amino acids (AA). In many attempts using chiral phases or after suitable derivatization it has been tried to separate the enantiomers by means of gas or liquid chromatography (for a review see Hare et al, 1985;Perrett, 1985;Engel and Hare, 1985;Brückner et al, 1991 and references therein).…”

Section: Introductionmentioning

confidence: 99%

Determination of L- and D-amino acids in foodstuffs by coupling of high-performance liquid chromatography with enzyme reactors

Voss

Galensa

2000

Amino Acids

View full text Add to dashboard Cite

A technique is described for the enantiomeric determination of L- and D-amino acids. It works on the principle that the separation efficiency of high-performance liquid chromatography is coupled with the specificity of enzymes and the sensitivity of electrochemical detection. After separation on a lithium cation-exchange column the amino acids are converted into keto acids and hydrogen peroxide under catalyzation of L- or D-amino acid oxidase. Hydrogen peroxide is detected amperometrically. The method has been tested by the analysis of beer, port, sherry, wine and fruit juice. A main emphasis was put onto the determination of D-alanine which can serve as an indicator for bacterial contamination. It is shown that a coupling of HPLC with enzyme reactors is a suitable technique for the rapid detection of this marker.

show abstract

Liquid Chromatography of Amino Acids and their Derivatives

Cited by 9 publications

References 147 publications

Characterization of aqueous two‐phase partition systems by distribution analysis of radiolabeled analytes (DARA): Application to process definition and control in biorecovery

Characterization of aqueous two‐phase partition systems by distribution analysis of radiolabeled analytes (DARA): Application to process definition and control in biorecovery

Archaeological Applications of Amino Acid Racemization*

Determination of L- and D-amino acids in foodstuffs by coupling of high-performance liquid chromatography with enzyme reactors

Contact Info

Product

Resources

About