2010
DOI: 10.1016/j.jchromb.2010.06.011
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Liquid chromatography–tandem mass spectrometric assay for clobetasol propionate in human serum from patients with atopic dermatitis

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Cited by 7 publications
(9 citation statements)
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“…on patients with atopic dermatitis (21), Olsen and Cornell found CP serum absorption of about 6–7 ng/ml through normal skin (19), while Sparidans et al. (22), validating the chemical assay for CP identification in human serum using HPLC–tandem mass spectrometry, measured CP blood concentrations of about 1 ng/ml in two patients with severe atopic dermatitis.…”
Section: Discussionmentioning
confidence: 99%
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“…on patients with atopic dermatitis (21), Olsen and Cornell found CP serum absorption of about 6–7 ng/ml through normal skin (19), while Sparidans et al. (22), validating the chemical assay for CP identification in human serum using HPLC–tandem mass spectrometry, measured CP blood concentrations of about 1 ng/ml in two patients with severe atopic dermatitis.…”
Section: Discussionmentioning
confidence: 99%
“…Finally, Spiridans et al. (22) described a chemical analytical assay to detect serum CP levels in two patients using CP ointment to treat severe atopic dermatitis.…”
Section: Introductionmentioning
confidence: 99%
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“…Serum clobetasol propionate levels CLO levels were measured using a validated LC/MS/ MS method in the range 0.05-10 ng/ml (9). In short, 500 ml serum, with clobetasone butyrate added as an internal standard, was extracted using hexanediethyl ether (3/1, v/v).…”
Section: Basal Serum Cortisol Levelsmentioning
confidence: 99%
“…It was also proven to be the first topical corticosteroid that demonstrated satisfactory results in the treatment for psoriasis 2,4 . Literature search reveals HPLC, RP-HPLC and liquid chromatography methods were reported for determination of various salts of Clobetasol in formulations like ointment, creams and suspensions [6][7][8][9][10][11] . Beside these, some simultaneous analytical estimations of Clobetasol 17-Propionate with other drugs have been reported in literature [12][13][14][15][16] .…”
Section: Introductionmentioning
confidence: 99%