Liquid chromatography tandem mass spectrometry with ion trap and triple quadrupole analyzers for determination of thyreostatic drugs in urine and muscle tissue

Woźniak, Barbara; Zuchowska, Iwona Matraszek; Żmudzki, J.; Jedziniak, Piotr; Korycińska, Beata; Sielska, Katarzyna; Witek, Sebastian; Kłopot, Alicja

doi:10.1016/j.aca.2011.02.021

Cited by 21 publications

(19 citation statements)

References 14 publications

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“…The previously published method for urine and muscle samples was used as a starting point for the development of this procedure (29). The positive ionisation was used in the method, as only this ionisation mode is suitable for all investigated compounds.…”

Section: Discussionmentioning

confidence: 99%

Determination of the Thyreostats in Animal Feeding Stuffs Using Liquid Chromatography-Tandem Mass Spectrometry

Woźniak

Witek

Matraszek-Żuchowska

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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A rapid liquid chromatography tandem mass spectrometry method was developed and validated to detect and confirm five thyreostatic drugs: tapazole, thiouracil, methylthiouracil, propylthiouracil, and phenylthiouracil in animal feeding stuff samples. Thyreostats were extracted from feed with methanol, and then degreasing of the extract with petroleum ether was performed, followed by the derivatisation of the compounds with 3-iodobenzylbromide in basic medium (pH 8.0). The derivatives were extracted with diethyl ether and analysed by gradient elution on a Poroshell 120-EC C18 column with triple quadrupole MS detection with turbo spray source in positive ionisation mode. The method was validated in accordance with the Commission Decision 2002/657/EC. For validation level of 10 μg kg -1 , the recovery ranged from 82% to 97.5% for all examined compounds. The repeatability and reproducibility did not exceed the limit of 20% for all analytes. The linearity was good for all thyreostats in the whole range of tested concentrations, as proved by the correlation coefficients greater than 0.99. The decision limits (CC) ranged from 1.63 μg kg -1 to 3.95 μg kg -1 , whereas the detection capabilities (CC) ranged from 2.74 μg kg -1 to 6.73 μg kg -1 . The developed analysis is sensitive and robust, and therefore useful for quantification and confirmation of thyreostats in residue control programme.

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Section: Discussionmentioning

confidence: 99%

Determination of the Thyreostats in Animal Feeding Stuffs Using Liquid Chromatography-Tandem Mass Spectrometry

Woźniak

Witek

Matraszek-Żuchowska

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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“…Some researchers have derivatised the ATDs with 3-iodobenzylbromide in order to stabilise them under single tautomeric form, to give them a non-polar behaviour and to increase their masses [10,38,40]. Other researchers have preferred the direct LC-MS detection in positive ion mode to avoid false identification [37] and to make the analysis of these substances simpler and faster when a high number of food or biological samples has to be controlled [34][35][36].…”

Section: Optimisation Of the Lc Conditions And Ms Detectionmentioning

confidence: 97%

“…On the other hand, GC has been used by coupling it to MS [25][26][27][28][29] and nitrogen-phosphorus detection [30]. Most LC methods have relied on UV-vis [31,32] and MS detection [33][34][35][36][37]10,[38][39][40]; some of the latters have included a preliminary derivatization step of the analytes in order to produce compounds with higher molecular weight and lower polarity, which are better retained on reversed phase (RP) columns and which generate a lower MS background resulting in higher S/N ratios [10,[38][39][40].…”

Section: Introductionmentioning

confidence: 99%

Analysis of antithyroid drugs in surface water by using liquid chromatography–tandem mass spectrometry

Pérez‐Fernández

Marchese

Gentili

et al. 2014

Journal of Chromatography A

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“…The analytical protocol describing the preparation of urine samples and LC-MS/MS analysis has been previously published (17). In brief, TU was extracted from urine samples with diethyl ether after derivatization with 3-iodobenzylbromide in basic medium, and detected and identified by means of LC-ESI (+)-MS/MS.…”

Section: Methodsmentioning

confidence: 99%

“…As a result, in some European countries, including Poland, one of thyreostatics -thiouracil (TU) is detected in urine samples of slaughtered animals (7). The examination of urine samples was performed by means of a liquid chromatography coupled with a triple quadrupole mass spectrometry -LC-MS/MS (17).…”

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confidence: 99%

Natural Occurrence of Thiouracil in Urine of Livestock in Poland

Woźniak

Witek

Żmudzki

et al. 2012

Bulletin of the Veterinary Institute in Pulawy

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Natural occurrence of thiouracil in bovine and swine urine in Poland was investigated. Under the national residue control programme, 537 urine samples were tested. In 77 samples (14.3%) thiouracil was detected above decision limit CCα (0.91 µg L -1 ), including eight samples over the recommended concentration of 10 µg L -1 . Of the bovine urine samples, 95 and 99 percentiles have thiouracil concentration below 4.50 and 14.85 μg L -1 ,and of porcine samples below 2.35 and 6.80 µg L -1 , respectively.

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Liquid chromatography tandem mass spectrometry with ion trap and triple quadrupole analyzers for determination of thyreostatic drugs in urine and muscle tissue

Cited by 21 publications

References 14 publications

Determination of the Thyreostats in Animal Feeding Stuffs Using Liquid Chromatography-Tandem Mass Spectrometry

Determination of the Thyreostats in Animal Feeding Stuffs Using Liquid Chromatography-Tandem Mass Spectrometry

Analysis of antithyroid drugs in surface water by using liquid chromatography–tandem mass spectrometry

Natural Occurrence of Thiouracil in Urine of Livestock in Poland

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