Liquid chromatography/tandem mass spectrometry assay for the quantification of troxerutin in human plasma

Liu, Fei; Yu, Xu; Lei, Rui; Gao, Shu; Dong, Haijun; Guo, Qi

doi:10.1002/rcm.2764

Cited by 21 publications

(27 citation statements)

References 9 publications

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“…This is because protein precipitation with perchloric acid does not remove water soluble impurities which would enhance the matrix effects. What is clear is that the assay reported here gives similar sensitivity to that as in [10] (LLOQ 0.01 ng mL -1 using 150 lL plasma vs. 0.025 ng mL -1 using 100 lL plasma) in a shorter cycle time (3.3 vs. 4.5 min) with improved accuracy and precision.…”

Section: Methods Validationsupporting

confidence: 52%

“…In the LC-MS-MS assay of troxerutin in human plasma reported by Liu et al [10], protein precipitation with perchloric acid produced variable matrix effects (93. and \9.03%, respectively. This is because protein precipitation with perchloric acid does not remove water soluble impurities which would enhance the matrix effects.…”

Section: Methods Validationmentioning

confidence: 99%

“…In particular, LC methods with fluorescence and ultraviolet-visible detection have very high values of the lower limit of quantitation (LLOQ) [8,9]. An LC-MS-MS method has been developed by Liu et al [10] involving sample preparation by protein precipitation with perchloric acid. The method gave adequate sensitivity (LLOQ 0.025 ng mL -1 ) for clinical studies in a short cycle time (4.5 min) but sample clean-up with perchloric acid was implicated in producing variable matrix effects.…”

Section: Introductionmentioning

confidence: 99%

“…A number of analytical methods for the determination of troxerutin in biological samples have been reported [8][9][10] but many suffer from disadvantages such as low sensitivity, relatively long analytical run time and complex sample preparation. In particular, LC methods with fluorescence and ultraviolet-visible detection have very high values of the lower limit of quantitation (LLOQ) [8,9].…”

Section: Introductionmentioning

confidence: 99%

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LC–MS–MS Determination of Troxerutin in Plasma and Its Application to a Pharmacokinetic Study

et al. 2010

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A highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for the determination of troxerutin in human plasma using tramadol as internal standard (IS) has been developed and validated. Sample preparation involved liquid-liquid extraction with ethyl acetate-isopropanol (95:5, v/v). The analyte and IS were separated by RP-LC with gradient elution using 10 mM ammonium acetate containing 0.1% formic acid and methanol at a flow rate of 0.9 mL min -1 . LC-MS-MS in the positive ion mode employed multiple reaction monitoring of the transitions at m/z 743.2?435.3 and m/z 264.1?58.0 for troxerutin and IS, respectively. The assay was linear in the concentration range 0.01-10 ng mL -1 with precision and accuracy within assay variability limits as per FDA guidelines. The assay was successfully applied to a pharmacokinetic study involving oral administration of 300 mg troxerutin to eight healthy Chinese volunteers.

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Section: Methods Validationsupporting

confidence: 52%

Section: Methods Validationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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LC–MS–MS Determination of Troxerutin in Plasma and Its Application to a Pharmacokinetic Study

et al. 2010

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“…A literature survey shows that, the relatively new Synergi Fusion-RP column employed in the present study has been in use since 2006 but has been utilized in only a few scientific studies. Two of those investigations 29,30 indicated that the Synergi column offered chromatographic benefits, and one 31 suggested that it has drawbacks compared to other columns, and the remaining four studies merely mentioned that the Synergi column had been used, without any further comments.…”

Section: Chromatographic Retardationmentioning

confidence: 99%

Investigation of sorption phenomena by solid phase extraction and liquid chromatography for the determination of some ether derivatives of tetrabromobisphenol A

Jonsson¹,

Hörsing²

2009

J of Physical Organic Chem

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To cite this version:Susanne Jonsson, Maritha Hörsing. Investigation of sorption phenomena by solid phase extraction and liquid chromatography for the determination of some ether derivatives of tetrabromobisphenol A. Journal of Physical Organic Chemistry, Wiley, 2009, 22 (11) AbstractObserved sorption and chromatographic behaviour served as a starting point for examination of four ether derivatives of the flame retardant tetrabromobisphenol (TBBPA); dihydroxyethyl (DHEE), dimethyl (DMe), diallyl (DAE) and dibromopropyl (DBPE). To date, there is little or no information about these compounds in the scientific literature. The targets in the present study had calculated log K ow values of 6.0-10. A broad range of different types of solvents were used in order to elucidate the mechanisms of sorption onto silica (glass) and two solid phase extraction cartridges based on polystyrene divinylbenzene polymers, hydroxylated (Isolute ENV+) and vinylpyrrolidone (Oasis HLB) containing units. Also, three chromatographic columns (C18, C8, and dual C18-based, i.e. a polar ligand together with C18 on a silica surface) were evaluated. The following were observed: desorption of the targets from silica surface by 1-propanol showed the best results and the yields were further improved by silylation of the surface. Regardless of the experimental test conditions applied, the lowest recovery was seen for the DMe derivative, followed by TBBPA itself, which had calculated log K ow values in between the other targets. A washing solution containing up to 70% of methanol could be used without elution of the targets from the . The dihydroxethyl ether derivative of TBBPA is most probably not affected by environmental relevant pH due to a likely high pKa (ca 15). Also, this study shows that the polar character of the dual-phase column was not as pronounced as asserted by the manufacturer.

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Current literature in mass spectrometry

2007

J. Mass Spectrom.

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252: 6404-7325. Devoe DL, Lee CS. Microfluidic technologies for MALDI-MS in proteomics. Electrophoresis 2006; 27: 3559. Hjerrild M, Gammeltoft S. Phosphoproteomics toolbox: Computational biology, protein chemistry and mass spectrometry. FEBS Lett 2006; 580: 4764. Kaltashov IA, Zhang MX, Eyles SJ, Abzalimov RR. Investigation of structure, dynamics and function of metalloproteins with electrospray ionization mass spectrometry. Anal Bioanal Chem 2006; 386: 472. Roe MR, Griffin TJ. Gel-free mass spectrometry-based high throughput proteomics: Tools for studying biological response of proteins and proteomes. Proteomics 2006; 6: 4678. Spitzer AR, Chace D. Mass spectrometry in neonatal medicine and clinical diagnostics -The potential use of mass spectrometry in neonatal brain monitoring. Clin Perinatol 2006; 33: 729. Tan DJL, Martinez Arias A. High-throughput localization of organelle proteins by mass spectrometry: A quantum leap for cell biology.

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Liquid chromatography/tandem mass spectrometry assay for the quantification of troxerutin in human plasma

Cited by 21 publications

References 9 publications

LC–MS–MS Determination of Troxerutin in Plasma and Its Application to a Pharmacokinetic Study

LC–MS–MS Determination of Troxerutin in Plasma and Its Application to a Pharmacokinetic Study

Investigation of sorption phenomena by solid phase extraction and liquid chromatography for the determination of some ether derivatives of tetrabromobisphenol A

Current literature in mass spectrometry

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