Liquid chromatography–tandem mass spectrometry determination of LSD, ISO-LSD, and the main metabolite 2-oxo-3-hydroxy-LSD in forensic samples and application in a forensic case

Johansen, Sys Stybe; Jensen, J. Langholm

doi:10.1016/j.jchromb.2004.12.040

Cited by 33 publications

(24 citation statements)

References 15 publications

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“…Therefore, the determination of various classes of LSD related drugs is important in many fields of analytical toxicology, such as forensic science, workspace drug testing, and antidoping analysis. Johansen and Jensen described a LC-ESI-MS/MS method for the determination of LSD, iso-LSD and 2-oxo-3-hydroxy-LSD in the forensic samples [60]. This method involves LLE for the extraction of analytes and LSD-D3 (internal standard) from 1.0 g of whole blood or 1.0 ml of urine using butyl acetate (pH 9.8).…”

Section: Determination Of Neurochemicals By Lc-ms Related Techniquesmentioning

confidence: 99%

Recent Advances in Mass Spectrometry for the Identification of Neurochemicals and their Metabolites in Biofluids

Kailasa

2013

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Recently, mass spectrometric related techniques have been widely applied for the identification and quantification of neurochemicals and their metabolites in biofluids. This article presents an overview of mass spectrometric techniques applied in the detection of neurological substances and their metabolites from biological samples. In addition, the advances of chromatographic methods (LC, GC and CE) coupled with mass spectrometric techniques for analysis of neurochemicals in pharmaceutical and biological samples are also discussed.

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Section: Determination Of Neurochemicals By Lc-ms Related Techniquesmentioning

confidence: 99%

Recent Advances in Mass Spectrometry for the Identification of Neurochemicals and their Metabolites in Biofluids

Kailasa

2013

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“…The most common buffers used for the extraction of basic drugs are Sörensen's buffer, [19] TRIS buffer [20] and borate buffer [21] and the most frequent organic solvents used for the LLE of drugs are TBME, [22] butyl acetate [23] and dichloromethane. [24] In the initial experiment, rilmenidine (1 ng/ml) was extracted from human serum, using nine different combinations of solvents and buffers.…”

Section: Sample Preparationmentioning

confidence: 99%

Development of a fast LC–MS/MS method for quantification of rilmenidine in human serum: elucidation of fragmentation pathways by HRMS

et al. 2010

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Rilmenidine is an alpha 2 adrenoreceptor agonist used in the treatment of mild and moderate hypertension. In this study, a fast and accurate liquid chromatographic method with tandem mass spectrometric detection has been validated in order to assure quantification of rilmenidine in human serum. The fragmentation pathway of protonated rilmenidine was studied using high-resolution mass spectrometry (HRMS). This study compared selectivity, linearity, accuracy, precision, extraction efficiency, matrix effect and sensitivity using common liquid-liquid extraction (LLE) and solid-phase extraction (SPE) procedures. The limit of quantitation for both extraction techniques was 0.1 ng/ml. Several differences between the LLE and SPE have been observed in terms of linearity, accuracy, precision and matrix effect. Additionally, the advantages of SPE included less manual work load and increased recovery of rilmenidine in human serum to approximately 80% (LLE, 57%). The developed method involving SPE was found to be accurate (relative error (RE) < 5%), reproducible (relative standard deviation, RSD < 7%), robust and suitable for quantitative analysis of rilmenidine in serum samples obtained from patients under antihypertensive treatment.

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“…According to a study by Peel and Boynton, LSD and its metabolites were detectable in urine samples from patients receiving 300 g of LSD for 34-120 h [9]. Since simultaneous determination of the parent drug and its metabolites assist in the interpretation of analysis, recent studies have included both LSD and O-H-LSD as suitable biomarkers for LSD intake [10][11][12][13][14]. When the timely collection of urine from drug suspects is impossible, hair has been used as an alternative.…”

Section: Introductionmentioning

confidence: 99%

“…With improvements in instrumental techniques, analytical methods employing mass spectrometry have been developed for the detection of LSD or/and its metabolites at low concentrations in various biological matrices [10][11][12][13][14][15][16][17]. However, studies on the analysis of LSD and its metabolites in hair are limited in comparison with those in urine or blood.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of LSD and 2-oxo-3-hydroxy LSD in hair and urine by LC–MS/MS and its application to forensic cases

Jang

Kim

Han

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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Liquid chromatography–tandem mass spectrometry determination of LSD, ISO-LSD, and the main metabolite 2-oxo-3-hydroxy-LSD in forensic samples and application in a forensic case

Cited by 33 publications

References 15 publications

Recent Advances in Mass Spectrometry for the Identification of Neurochemicals and their Metabolites in Biofluids

Recent Advances in Mass Spectrometry for the Identification of Neurochemicals and their Metabolites in Biofluids

Development of a fast LC–MS/MS method for quantification of rilmenidine in human serum: elucidation of fragmentation pathways by HRMS

Simultaneous determination of LSD and 2-oxo-3-hydroxy LSD in hair and urine by LC–MS/MS and its application to forensic cases

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