Liquid chromatography–tandem mass spectrometry quantification of levosulpiride in human plasma and its application to bioequivalence study

Phapale, Prasad; Lee, Hae Won; Lim, Myung-Seop; Seong, Sook Jin; Kim, Eun‐Hee; Park, Jeong-Hyeon; Lee, Mi Ran; Hwang, Sung-Kyu; Yoon, Young‐Ran

doi:10.1016/j.jchromb.2010.06.036

Cited by 9 publications

(5 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, QqQ instruments comply with the regulatory requirements of accuracy, precision, specificity, and sensitivity for bioanalytical method validation. The method validation can be performed as per guidelines to establish limits of quantification and detection, dynamic range, recovery, matrix effect, and stability 19,20 . The current bioanalytical methods are however restricted to the measurement of a single drug analyte and its internal standard from biofluid samples.…”

Section: Pk and Targeted Metabolomics Using Qqqmentioning

confidence: 99%

“…The method validation can be performed as per guidelines to establish limits of quantification and detection, dynamic range, recovery, matrix effect, and stability. 19 , 20 The current bioanalytical methods are however restricted to the measurement of a single drug analyte and its internal standard from biofluid samples. It should be noted that the same biofluid with similar sample preparation does contain a wealth of information about endogenous metabolites (metabolome).…”

Section: Pk and Targeted Metabolomics Using Qqqmentioning

confidence: 99%

See 1 more Smart Citation

Pharmaco‐metabolomics opportunities in drug development and clinical research

Phapale

2021

Analytical Science Advances

View full text Add to dashboard Cite

Pharmaco‐metabolomics uses metabolic phenotypes for the prediction of inter‐individual variations in drug response and helps in understanding the mechanisms of drug action. The field has made significant progress over the last 14 years with numerous studies providing clinical evidence for personalised medicine. However, discovered pharmaco‐metabolomic biomarkers are not yet translated into clinics due to a lack of large‐scale validation. Integration of targeted and untargeted metabolomics workflows into pharmacokinetic analysis and drug development can advance the field from bench to bedside. Also, Indian pharmaceutical research and its bioanalytical infrastructure are in a position to take on these opportunities by addressing challenges such as appropriate training and regulatory compliance.

show abstract

Section: Pk and Targeted Metabolomics Using Qqqmentioning

confidence: 99%

Section: Pk and Targeted Metabolomics Using Qqqmentioning

confidence: 99%

Pharmaco‐metabolomics opportunities in drug development and clinical research

Phapale

2021

Analytical Science Advances

View full text Add to dashboard Cite

show abstract

“…As per literature, several liquid chromatography tandem mass spectrometry (LC–MS/MS) methods are reported for the estimation of esomeprazole [15] , [16] , [17] , rabeprazole [18] , [19] , [20] , [21] , [22] and levosulpiride [23] , [24] , [25] individually in biological samples. Till date, there is no ultra pressure liquid chromatography–mass spectrometry (UPLC–MS/MS) method available for the estimation of esomeprazole, rabeprazole and levosulpiride simultaneously in human plasma.…”

Section: Introductionmentioning

confidence: 99%

“…Another method reported by Huang et al [20] for the quantification of rabeprazole in human plasma, has utilizes very small amount of plasma for sample preparation, but this method also requires longer chromatographic run time which is not suitable for high throughput sample analysis. Phapale et al [23] reported the LC–MS/MS method for quantification of levosulpiride in human plasma, but it has long chromatographic runtime compared to the current proposed UPLC method.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a high throughput UPLC–MS/MS method for simultaneous quantification of esomeprazole, rabeprazole and levosulpiride in human plasma

Chunduri

Dannana

2016

Journal of Pharmaceutical Analysis

View full text Add to dashboard Cite

A high throughput ultra pressure liquid chromatography–mass spectrometry (UPLC–MS/MS) method with good sensitivity and selectivity has been developed and validated for simultaneous quantification of esomeprazole, rabeprazole and levosulpiride in human plasma using lansoprazole as internal standard (IS). The extraction method based on liquid–liquid extraction technique was used to extract the analytes and IS from of 50 µL of human plasma using methyl tert-butyl ether:ethyl acetate (80:20, v/v), which offers a high recovery. Chromatographic separation of analytes and IS was achieved on a Hypersil gold C18 column using gradient mobile phase consisting of 2 mM ammonium formate/acetonitrile. The flow rate was set at 0.5 mL/min to elute all the analytes and IS within 1.00 min runtime. Detection of target compounds was performed on a triple quadruple mass spectrometer by multiple reaction monitoring (MRM) mode via positive electrospray ionization (ESI). Method validation results demonstrated that the developed method has good precision and accuracy over the concentration ranges of 0.1–2000 ng/mL for each analyte. Stability of compounds was established in a battery of stability studies, i.e., bench top, autosampler, dry extract and long-term storage stability as well as freeze-thaw cycles. The validated method has been successfully applied to analyze human plasma samples for application in pharmacokinetic studies.

show abstract

“…Bioana lysts were quick to realize the benefits of this new particle LC technology. Many authors showed how the smaller chromatographic particles gave rise to more sensitive assays [8,9], sometimes up to tenfold [10,11], and faster ana lysis [12][13][14]. Latterly the benefits of the extra resolution afforded by these materials were highlighted by Houghton and Grace.…”

mentioning

confidence: 99%

Rapid Analysis of Dried Blood Spot Samples with Sub-2-µM LC–MS/MS

et al. 2011

View full text Add to dashboard Cite

The use of dried blood and dried plasma spots for storage and transportation of samples derived from clinical trials holds the promise to reduce cost, simplify storage and shipping as well as reducing animal usage. From the bioanalysts' point of view, these dried-paper samples add an extra layer of complexity to the analysis introducing extra matrix effects from the paper itself and sometimes from antiviral treatments applied to the card. In this article we demonstrate the use of the sub-2-µm particle LC-MS/MS for the bioanalysis of samples derived from a dried blood spot. The higher resolution provided by these small-particle separations allowed for greater resolution of the analyte from the endogenous components in blood samples and from the card-treatment chemicals. The method-development process was enhanced by the use of MS, which could simultaneously acquire full scan and multiple reaction monitoring data, allowing resolution from metabolites and endogenous matrix components. The use of this approach produced sensitivity levels in the 50-100 pg/ml range and analysis times in the 1-2 min range, which was five-times more sensitive and three-times faster than HPLC. This throughput and sensitivity makes this approach ideal for the analysis of preclinical and clinical studies derived from dried blood spots.

show abstract

Liquid chromatography–tandem mass spectrometry quantification of levosulpiride in human plasma and its application to bioequivalence study

Cited by 9 publications

References 23 publications

Pharmaco‐metabolomics opportunities in drug development and clinical research

Pharmaco‐metabolomics opportunities in drug development and clinical research

Development and validation of a high throughput UPLC–MS/MS method for simultaneous quantification of esomeprazole, rabeprazole and levosulpiride in human plasma

Rapid Analysis of Dried Blood Spot Samples with Sub-2-µM LC–MS/MS

Contact Info

Product

Resources

About