Liquid Chromatography Tandem Mass Spectrometry Determination Method of Bencycloquidium Bromide: Application to Drug Interaction Study in Human

Agbokponto, Janvier Engelbert; Yemoa, Loconon Achille; Assanhou, Assogba Gabin; Liu, Ruijuan; Ganfon, Habib; Ding, Li

doi:10.22159/ijpps.2021v13i10.5791

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…To every calibration standard solution, 50 μl of internal standard solution with concentration of EMPA (100 pg/ml) was added. All the solutions were stored at-80 °C and prior to analysis they are brought to ambient temperature [26].…”

Section: Calibration Standard Solutionsmentioning

confidence: 99%

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Pharmacokinetic Investigation of Remogliflozin in Rat Plasma Samples by High-Throughput HPLC-MS-MS

2022

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Objective: Remogliflozin (REMO), a selective inhibitor of the renal sodium-dependent glucose transporter 2 channel, which could increase urine glucose excretion and lower plasma glucose in humans. To establish a simple, sensitive and completely validated HPLC-MS-MS approach for the analysis of Remogliflozin in rat plasma samples. Methods: The method was developed after simple step protein precipitation by acetonitrile and Empagliflozin (EMPA) was used as internal standard. Separation was done on an CORTECS C18, 90 Å, 2.7 µm, 4.6 mm X 150 mm with an isocratic mobile phase consisting of 0.1% Formic acid: acetonitrile (20:80%, v/v) and pumped at a flow stream of 0.8 ml/min at ambient temperature. Results: The approach developed showed fine calibration curve in the quantity range of 5-1000 pg/ml with correlation coefficient (r2) of ≥ 0.9997 and the intra-run accuracy and precision was 99.91 to 109.07% and 0.17 to 1.34, inter-run accuracy and precision was 99.8 to 101.54 and 0.17 to 1.66 according to FDA guidelines. Conclusion: The newly designed and validated approach was simple, fast and applied effectively for single-dose oral pharmacokinetic investigation in Wistar male rats for the quantification of REMO in biological matrix.

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Section: Calibration Standard Solutionsmentioning

confidence: 99%

“…Samples of quality control were made as explained above in the similar way at concentrations corresponding to 5 pg/ml (LLOQ), 15 pg/ml (LQC), 500 pg/ml (MQC) and 800 pg/ml (HQC). All the solutions were stored at-80 °C and prior to analysis they are brought to ambient temperature [26].…”

Section: Quality Control Samplesmentioning

confidence: 99%

Pharmacokinetic Investigation of Remogliflozin in Rat Plasma Samples by High-Throughput HPLC-MS-MS

2022

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Determination of Pharmacokinetic Parameters From Distribution Study Following Development and Validation of a Sensitive Lc-MS Method in Tissue Matrices for 2-(4-Ethoxyphenylsulphonamido) Pentanediamide, an Investigational Anticancer Agent

YEASMIN,

MISHRA,

SEN

2024

Int J App Pharm

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Objective: The aim of this study was to develop and validate bioanalytical methods for estimation of 2-(4-ethoxyphenyl sulphamido) pentanediamide (PC), an investigational anticancer agent, in various organ/tissue matrices to study various Pharmacokinetic parameters using lC-MS. Methods: Freshly prepared tissue homogenates from Sprague-Dawley rats were used as matrices to develop the bioanalytical method in lC-MS to determine Cmax, Tmax, AUC0-t, AUC0-inf, T1/2, and mean Residence Time (MRT). The distribution study was conducted by administering PC orally to Sprague-Dawley rats and quantifying PC in different excised organs at different points. A non-compartmental analysis was done using ‘PK solver’ software. Results: In all the tissue matrices, the concentrations of PC were found in the linear range of 10 to 5000 ng/ml. High level of precision, accuracy, and recovery, with negligible matrix effects, were found. PC was distributed in all tissues except the brain. Pharmacokinetic parameters such as Tmax and MRT were between 1.11±0.12 to 2.33±0.11 h and 2.17±0.16 to 4.01±0.25 h respectively in the liver, lung, heart, spleen, kidney, and thymus. Conclusion: Simple and sensitive lC-MS methods for PC in different tissue matrices were developed and validated. As PC does not cross Blood Brain Barrier (BBB), it will not adversely affect Central Nervous System (CNS). PC is absorbed fast from Gastro Intestinal Tract (GIT) to blood and subsequently reaches the different tissues. Consequently, a fast onset of action will be seen. To sum up, PC is a probable potential anticancer agent with no or minimal adverse effects on CNS.

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Optimization of Lc-MS/MS Method for the Simultaneous Determination of Metformin and Rosiglitazone in Human Plasma With Box-Behnken Design

KAUSER,

PADAVALA,

PALANIVEL

2024

Int J App Pharm

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Objective: The objective of this study was to develop a robust Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS) methodology for the precise quantification of metformin and rosiglitazone in human plasma. Methods: A Design of Experiments (DOE) framework was utilized, specifically employing a Box-Behnken experimental design, to optimize critical parameters such as Capillary voltage, Cone voltage, Desolvation temperature, and Collision energy. Sample preparation involved protein precipitation using acetonitrile, simplifying the procedure. Chromatography was performed with a mobile phase of 0.1% formic acid and acetonitrile (60:40 V/V) to enhance sensitivity and reproducibility. Quantification was achieved through Multiple Reaction Monitoring (MRM) of the transition’s m/z 130.1 → m/z 60.1 for metformin, m/z 358.2 → m/z 134.9 for rosiglitazone, and m/z 206.3 → m/z 59.9 for phenformin. The methodology was validated according to regulatory guidelines. Results: The developed methodology demonstrated selectivity, linearity, accuracy, precision, recovery, and stability. The calibration curve showed linearity over the concentration range of 5 ng/ml to 1000 ng/ml for metformin and 1.5 ng/ml to 300 ng/ml for rosiglitazone. Accuracy and precision were within acceptable limits across calibration and quality control standards. Assessments of extraction recovery and matrix effects confirmed the robustness of the extraction procedure, with negligible interference from plasma components. Stability studies indicated that the method maintained acceptable limits for metformin and rosiglitazone concentrations under various storage and handling conditions. Conclusion: The validated Liquid Chromatography – Tandem Mass Spectrometry (LC-MS/MS) methodology provides a reliable and accurate platform for the quantification of metformin and rosiglitazone in human plasma. This method shows potential applications in pharmacokinetic studies and clinical research, ensuring consistent performance in routine analysis.

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Liquid Chromatography Tandem Mass Spectrometry Determination Method of Bencycloquidium Bromide: Application to Drug Interaction Study in Human

Cited by 3 publications

References 10 publications

Pharmacokinetic Investigation of Remogliflozin in Rat Plasma Samples by High-Throughput HPLC-MS-MS

Pharmacokinetic Investigation of Remogliflozin in Rat Plasma Samples by High-Throughput HPLC-MS-MS

Determination of Pharmacokinetic Parameters From Distribution Study Following Development and Validation of a Sensitive Lc-MS Method in Tissue Matrices for 2-(4-Ethoxyphenylsulphonamido) Pentanediamide, an Investigational Anticancer Agent

Optimization of Lc-MS/MS Method for the Simultaneous Determination of Metformin and Rosiglitazone in Human Plasma With Box-Behnken Design

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