Liquid chromatography with absorbance detection and with isotope-dilution mass spectrometry for determination of isoflavones in soy standard reference materials

Phillips, Melissa M.; Bedner, Mary; Reitz, Manuela; Burdette, Carolyn Q.; Nelson, Michael A.; Yen, James H.; Sander, Lane C.; Rimmer, Catherine A.

doi:10.1007/s00216-016-9997-7

Cited by 9 publications

(7 citation statements)

References 37 publications

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“…Two Official Methods of Analysis have been developed by AOAC International for the determination of isoflavones in soy beans, dietary supplements, and soy foods: the AOAC Official Method 2001.10 (AOAC ), based on extraction, alkaline hydrolysis, and liquid chromatography with UV detection (LC‐UV), for the determination of isoflavone glycosides and aglycones; and the AOAC Official Method 2008.03 (AOAC ), based on extraction and LC‐UV, for the determination of isoflavone glycosides, malonyl‐, and acetyl‐glycosides, and aglycones. Recently, Phillips and others () developed 2 analytical methods, based on liquid chromatography with absorbance detection and with isotope‐dilution mass spectrometry, for the determination of 6 isoflavones (Di, Gi, Gly, De, Ge, Gle) in soy standard reference materials (SRMs). Both methods were based on basic hydrolysis to cleave malonyl‐ and acetyl‐glycosides and an internal standard approach for quantification.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Isoflavones in Foods

Bustamante-Rangel

Delgado-Zamarreño

Pérez‐Martín

et al. 2018

Comp Rev Food Sci Food Safe

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In recent years the nutritional and bioactive properties of foods are being intensively investigated with a view to control, in addition to food quality, their possible influence on human health. Because of this, there is a growing demand for rapid, selective, sensitive, and validated methods for analysis and quantification. Bioactive plant compounds include those with weak estrogenic activity (phytoestrogens), among which are the isoflavones. Some of the beneficial activities that have been attributed to isoflavones are anticarcinogenic activity, the prevention of cardiovascular disease, the improvement of bone health, and antioxidant activity. The objective of this work is to provide an updated review of the methods used in sample preparation and subsequent analysis for the determination of isoflavones in food samples, including both soybean and soy products, as well as other foods with low isoflavone contents. The review focuses on the most common sample preparation techniques used during the last 10 years, including both conventional solvent extraction and other more recent extraction techniques. Separation and detection methods, including current trends in liquid chromatography analysis, such as the use of monolithic columns or ultra-high-pressure liquid chromatography, are also discussed.

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Section: Introductionmentioning

confidence: 99%

Analysis of Isoflavones in Foods

Bustamante-Rangel

Delgado-Zamarreño

Pérez‐Martín

et al. 2018

Comp Rev Food Sci Food Safe

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“…Phillips et al [13] described the use of two novel independent methods for the determination of isoflavones in four different soybased dietary supplement SRMs. Different sample preparation approaches were optimized followed by analysis using LC with absorbance detection and ID LC-MS for the determination of six isoflavones in soy flour, soy protein isolate, soy protein concentrate, and soy-containing oral solid dosage form.…”

Section: Novel Methodsmentioning

confidence: 99%

“…Different sample preparation approaches were optimized followed by analysis using LC with absorbance detection and ID LC-MS for the determination of six isoflavones in soy flour, soy protein isolate, soy protein concentrate, and soy-containing oral solid dosage form. Phillips et al [13] compared the results from the two analytical methods, which also contributes to the analytical novelty, and described the combination of results to obtain the certified values.…”

Section: Novel Methodsmentioning

confidence: 99%

What is novel about certified reference materials?

Wise

2018

Anal Bioanal Chem

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“…An avocado material (SRM 2386) is in progress as a high-fat, powdered matrix located in sector 2. Two soy-related SRMs (SRM 3236 soy protein isolate and SRM 3237 soy protein concentrate) [46], which were developed for isoflavone measurements, reside in sector 9 (not shown), and they could be useful protein-rich matrices, if additional characterization for nutrients is performed. Existing food-matrix CRMs for trace elements only (e.g., wheat and rice flour, bovine and porcine liver, spinach, etc.)…”

Section: Future Crm Needs For Organic Nutrientsmentioning

confidence: 99%

Evolution of reference materials for the determination of organic nutrients in food and dietary supplements—a critical review

Wise

Phillips

2018

Anal Bioanal Chem

Self Cite

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For over 40 years, food-matrix certified reference materials (CRMs) have been available for determination of trace element content, and a wide variety of materials are available from most producers of CRMs. However, the availability of food-matrix CRMs for organic nutrients has been more limited. The European Commission (EC) Bureau Communautaire de Référence (BCR) and the National Institute of Standards and Technology (NIST) introduced food-matrix CRMs with values assigned for vitamins and other organic nutrients such as fatty acids and carotenoids in the 1990s. The number of organic nutrients for which values were assigned has increased significantly in the past decade, and the approach and analytical methods used for assignment of the certified values have also evolved. Recently, dietary supplement-matrix CRMs such as multivitamin tablets with values assigned for vitamins and carotenoids, and fish and plant oils with values assigned for fatty acids have appeared. The development, evolution, and improvement of food- and dietary supplement-matrix CRMs for determination of vitamins, carotenoids, and fatty acids are described, with emphasis on CRMs made available in the past 10 years. Recent food and dietary supplement CRMs for the determination of organic nutrients include infant formula, multivitamin tablets, milk and egg powders, breakfast cereal, meat homogenate, blueberries, soy flour, fish and plant oils, dry cat food, and protein drink powder. Many of these food- and supplement-matrix CRMs have values assigned for over 80 organic and inorganic nutrients, toxic elements, proximates, and contaminants. The review provides a critical assessment of the challenges and evolving improvements in the production and the analytical methods used for value assignment of these CRMs. The current status and future needs for additional food- and dietary supplement-matrix CRMs for organic nutrients are also discussed.

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Liquid chromatography with absorbance detection and with isotope-dilution mass spectrometry for determination of isoflavones in soy standard reference materials

Cited by 9 publications

References 37 publications

Analysis of Isoflavones in Foods

Analysis of Isoflavones in Foods

What is novel about certified reference materials?

Evolution of reference materials for the determination of organic nutrients in food and dietary supplements—a critical review

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