Liquid crystal-based sensitive and selective detection of uric acid and uricase in body fluids

Cheng, Supan; Khan, Mashooq; Yin, Fangchao; Wu, Wen‐Li; Sun, Tao; Hu, Qiongzheng; Lin, Jin‐Ming; Wang, Xiao

doi:10.1016/j.talanta.2022.123455

Cited by 11 publications

(6 citation statements)

References 29 publications

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“…UA and cTn are typical biomarkers for gout and AMI, respectively. [42][43][44][45][46][47] However, the goldstandard methods used in clinic cannot be used to give rapid results in the home. Together with a homemade centrifugal device and hand-held Raman spectrometer, the 3D-graphene/Al-AAO substrate was used to create a home detection system to rapidly detect the amount of UA and cTn in the blood of a patient.…”

Section: Resultsmentioning

confidence: 99%

A synergistic biomimetic optical structure for household health monitoring

Zhang

Ding

et al. 2023

Preprint

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A breakthrough in the performance of bionic optical structures will only be achieved if we can obtain an in-depth understanding of the synergy mechanisms operating in natural optical structures and find ways to imitate them. In this work, inspired by feline eyes, an optical structure that takes advantage of a synergistic effect that occurs between resonant and reflective structures was designed. The reflective structure consists of anodic aluminum oxide with an aluminized inner layer (Al-AAO), and the resonant structure consists of three-dimensional (3D) graphene inside, and on the surface of the Al-AAO. The synergistic effect between the reflective and resonant components leads to a Raman enhancement factor (EF) of 1.16 × 107 which is much greater than that achieved using the reflective/resonant cavities on their own. A 2-3 order of magnitude increase in sensitivity could thus be achieved when used to detect model compounds. More importantly, the optical device was further used to develop a highly-sensitive household health monitoring system. The system uses simple apparatus (homemade centrifugal device and hand-held Raman spectrometer) and rapidly produces results (detection time＜3 min). It can thus be used to give early warning of acute diseases with high risk (e.g., acute myocardial infarction). The 3D-graphene/Al-AAO substrates were also found to have good reusability and storability (9% and 7% reduction in EF after washing 30 times and 8 weeks of storage, respectively). They thus reduce detection costs (to ~$1), making them much cheaper to use than the current gold-standard methods (e.g., ~$16 for gout detection).

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Section: Resultsmentioning

confidence: 99%

A synergistic biomimetic optical structure for household health monitoring

Zhang

Ding

et al. 2023

Preprint

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“…For example, abnormal levels of uric acid or uricase can be correlated to diseases such as diabetes and leukemia and to kidney damage. It was shown that surfactant-decorated aqueous interfaces of LCs could be fabricated to detect uric acid and uricase in human urine samples with sensitivity comparable to that of the standard calorimetric method . Similarly, LC–aqueous interfaces were also tailored for the detection of α-synuclein and cholic acid, which are the clinical biomarkers for the diagnosis of Parkinson’s disease and liver diseases, respectively. − …”

Section: Detection Of Pathologies In Clinical Samplesmentioning

confidence: 99%

Liquid Crystal Biosensors: A New Therapeutic Window to Point-of-Care Diagnostics

2023

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After revolutionizing the field of electro-optic displays, liquid crystals (LCs) are emerging as functional soft materials with wide-ranging biomedical implications. Integrating smart sensor designs with label-free imaging presents exciting opportunities in diagnostics. In this Perspective, we present an elegant collage of the key findings that demonstrate the utility of LC biosensors in diagnosing a disease or infection in clinical samples, cellular microenvironments, or bodily fluids. We emphasize the currently prevalent diagnostic techniques and the advances made using LCs in achieving greater sensitivity, a simplified strategy, multiplexed detection, and so on. We collate the landmark contributions in translational research in LC-based diagnostics. We believe that developing LC-based biosensors presents a new therapeutic window in point-of-care diagnostics.

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“…Based on the nature of the interface where the specific LC–analyte interaction occurs, LC-based biosensors are broadly classified into three categories: LC–solid, LC–aqueous and LC–in-water emulsion . It has been shown that LC-based biosensors have competitive advantages (e.g., time and cost-efficiency) over conventional biosensors in detecting toxins, − glucose, − small molecules, , amino acids, − DNA, − and disease biomarkers. − However, LC-based direct monitoring of structural changes in protein molecules has yet to be developed, despite its importance in biosensing. This motivates the present study to develop a simple LC–aqueous biosensor capable of assessing the extent of protein unfolding and refolding quantitatively both off-line and in real-time.…”

Section: Introductionmentioning

confidence: 99%

Harnessing Liquid Crystal Sensors for High-Throughput Real-Time Detection of Structural Changes in Lysozyme during Refolding Processes

Guo,

Zhao,

et al. 2023

Anal. Chem.

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Despite the rapid advances in process analytical technology, the assessment of protein refolding efficiency has largely relied on off-line protein-specific assays and/or chromatographic procedures such as reversed-phase high-performance liquid chromatography and size exclusion chromatography. Due to the inherent time gap pertaining to traditional methods, exploring optimum refolding conditions for many recombinant proteins, often expressed as insoluble inclusion bodies, has proven challenging. The present study describes a novel protein refolding sensor that utilizes liquid crystals (LCs) to discriminate varying protein structures during unfolding and refolding. An LC layer containing 4-cyano-4′-pentylbiphenyl (5CB) intercalated with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) is used as a sensing platform, and its proof-of-concept performance is demonstrated using lysozyme as a model protein. As proteins unfold or refold, a local charge fluctuation at their surfaces modulates their interaction with zwitterionic phospholipid DOPE. This alters the alignment of DOPE molecules at the aqueous/LC interface, affecting the orientational ordering of bulk LC (i.e., homeotropic to planar for refolding and planar to homeotropic for unfolding). Differential polarized optical microscope images of the LC layer are subsequently generated, whose brightness directly linked to conformational changes of lysozyme molecules is quantified by gray scale analysis. Importantly, our LC-based refolding sensor is compatible with diverse refolding milieus for real-time analysis of lysozyme refolding and thus likely to facilitate the refolding studies of many proteins, especially those lacking a method to determine structure-dependent biological activity.

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Liquid crystal-based sensitive and selective detection of uric acid and uricase in body fluids

Cited by 11 publications

References 29 publications

A synergistic biomimetic optical structure for household health monitoring

A synergistic biomimetic optical structure for household health monitoring

Liquid Crystal Biosensors: A New Therapeutic Window to Point-of-Care Diagnostics

Harnessing Liquid Crystal Sensors for High-Throughput Real-Time Detection of Structural Changes in Lysozyme during Refolding Processes

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