Liquid crystalline lipid nanoparticle promotes the photodynamic activity of gallium protoporphyrin against S. aureus biofilms

Awad, Muhammed; Barnes, Timothy J.; Joyce, Paul; Thomas, Nicky; Prestidge, Clive A.

doi:10.1016/j.jphotobiol.2022.112474

Cited by 10 publications

(52 citation statements)

References 48 publications

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“…LCNP was prepared using the hydrotrope dilution method, as previously described [ 8 , 16 ]. Briefly, glycerol monooleate (15 mg) were mixed with Pluronic F127 in powder form (3 mg) and propylene glycol (0.26 g) via vortexing for 2 min in a 15 mL glass vial to form a homogenous gel, followed by the addition of 0.5 mL methanolic solution of gallium protoporphyrin (GaPP) (1.5 mM).…”

Section: Methodsmentioning

confidence: 99%

“…The light source used is a mounted LED at 405 nm wavelength (M405L4) [ 8 ]. An aspheric condenser lens, Ø1”, f = 16 mm, NA=0.79, ARC: 350–700 nm, was attached to the mounted LED using an SM1 Lens Tube, 1.00” thread depth to collimate the light beam to illuminate an area with a diameter of 2 cm, sufficient to illuminate four wells at time.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, gallium protoporphyrin (GaPP) has been proposed as a photosensitizer against S. aureus [ 8 , 9 ]. GaPP structural similarity with heme enables its superior uptake by S. aureus , compared to other porphyrins through heme acquisition pathways [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…GaPP structural similarity with heme enables its superior uptake by S. aureus , compared to other porphyrins through heme acquisition pathways [ 9 , 10 ]. This uptake mechanism potentiates its antibacterial activity against S. aureus as iron mimetic agent in the dark and as photosensitizer upon light activation [ 8 ]. However, not all bacterial species are equally sensitive to GaPP as heme mimetic [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Previously we have demonstrated the positive impact of liquid crystal lipid nanoparticles (LCNP) on the photodynamic activity of GaPP. Through optimizing GaPP-LCNP formulation, a significant improvement in the capabilities of GaPP as an iron mimetic agent and photosensitizer was evidenced by enhanced delivery of GaPP into S. aureus biofilms and higher 1 O 2 quantum yield upon light activation [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Gallium Protoporphyrin Liquid Crystalline Lipid Nanoparticles: A Third-Generation Photosensitizer against Pseudomonas aeruginosa Biofilms

et al. 2022

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The looming antimicrobial resistance pandemic has encouraged the investigation of antimicrobial photodynamic therapy (aPDT) as a promising technology to combat recalcitrant bacterial infections caused by antibiotic resistant strains. Here, we report on the optimization and effective application of gallium protoporphyrin liquid crystalline lipid nanoparticles (GaPP-LCNP) as a photosensitizer for aPDT against the Gram-negative bacteria P. aeruginosa in both planktonic and biofilm modes of growth. LCNP significantly enhanced the performance of GaPP as photosensitizer by two-fold, which was correlated with higher antibacterial activity, reducing the viability of planktonic P. aeruginosa by 7 log10 using 0.8 µM GaPP-LCNP and a light dose of 17 J.cm–2. Importantly, GaPP-LCNP also reduced the viability of biofilms by 6 log10 at relatively low light dose of 34.2 J.cm-2 using only 3 µM GaPP-LCNP. The high antibiofilm activity of GaPP-LCNP at low GaPP-LCNP dose indicated the high efficiency and safety profile of GaPP-LCNP as a promising platform for photodynamic inactivation of recalcitrant infections.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%