Liquid Inoculation with Rhizobia in the Planting Furrow of Common Bean under No‐Till Is Feasible under Different Soil and Climatic Conditions

Abstract

Common bean (Phaseolus vulgaris L.) producers that have adopted a high level of technology have shown interest in the use of liquid inoculant in the planting furrow, especially because of the practicality of this operation in large‐scale planting operations. However, consistent information on this practice is lacking for common bean, which requires studies to assist the producer in the choice of the inoculation method to be adopted and in determination of the application rates adequate for good performance of biological N2 fixation. To that end, two field experiments were conducted under the no‐till system under different soil and climatic conditions, both of them in irrigated areas. A randomized block experimental design was used, with four replications and seven treatments (three rates of liquid inoculation in the planting furrow; peat‐based or liquid inoculation on the seed; and two control treatments without inoculation, without and with N‐urea). The cultivar used was BRSMG Madrepérola and the rhizobial strain was CIAT 899 (±109 cells of Rhizobium tropici per milliliter or gram). Inoculation on the seed was performed shortly before sowing. Inoculation in the planting furrow at the rate of 3.5 L ha−1 not only leads to higher yields than inoculations on the seed but also ensures bean seed yields equivalent to those of the treatment with mineral N. Therefore, due to operational practicality, inoculation in the planting furrow is the most advantageous of the inoculations tested, regardless of the soil and climatic conditions in this study.

Selection of elite Rhizobium strains by biometric techniques for inoculation in common bean

“…Even numerous and competitive native soil bacterial populations are not always able to establish efficient symbiosis that significantly contributes to common bean growth. Thus, it may be necessary to employ inoculants containing strains that are more efficient in N 2 fixation (Ferreira et., 2009; Moreira, Siqueira & Brussaard, 2006; Oliveira et al., 2019; Oliveira, Ferreira, et al., 2018; Oliveira, Soares, et al., 2018; Pádua Oliveira et al., 2017; Soares et al., 2006), such as those found in the present study (Tables 2 and 4; Figures 2, 3, and 4). The contribution of these populations rises, in turn, when complemented with 80 kg N‐urea ha −1 (C7), that is, the increase in yield was due to the nitrogen fertilizer; however, C7 showed less stability than C6.…”

Contribution of Biofertilizers to Pulse Crops: From Single-Strain Inoculants to New Technologies Based on Microbiomes Strategies

“…Confirming this information, most of the rhizobial inoculants tested in the recent literature belong to the genus Rhizobium , such as R. etli [ 51 ], R. leguminosarum [ 52 ], and R. tropici [ 51 , 53 , 54 , 55 ]. However, inoculation did not always result in a positive response, and in some cases, productivity decreased [ 54 ].…”

“…Productivity varied between countries, with some producing hundreds of kg [ 35 ], while others a few thousand kg per ha [ 51 , 53 , 55 , 56 , 57 ]. For example, Oliveira et al [ 51 ] obtained 1975 kg ha −1 with the inoculation of R. tropici , which was comparable to the control with 80 kg N ha −1 . This variation is probably due to local conditions and variations in plant genetic material and the technological levels.…”

Сучасні Системи Проведення Інокуляції Посівного Матеріалу Просапних Культур

“…Водночас, для мікроорганізмів, що містяться в інокулянтах температура також відіграє важливу роль і повинна бути в межах 10-30℃ (Welver, 2019). Наразі, досліджуються нові варіанти інокуляції насіння, тобто безпосередньо в борозні (Schiffmann & Alper, 1968;Rezende et al, 2016;Oliveira et al, 2019).…”