Liquid–liquid extraction of biomolecules: an overview and update of the main techniques

Mazzola, Priscila Gava; Lopes, André Moreni; Hasmann, Francislene A.; Jozala, Ângela Faustino; Penna, Thereza Christina Vessoni; Magalhães, Pérola Oliveira; Rangel-Yagui, Carlota de Oliveira

doi:10.1002/jctb.1794

Cited by 225 publications

(131 citation statements)

References 134 publications

(140 reference statements)

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“…To a large extent, interest in the partitioning behavior of proteins and other biomolecules in ATPSs have been stimulated by the potential of these systems to provide innocuous environments for the treatment of biological materials [1][2][3][4][5][6]. It is an environmentally friendly and easy to scale-up technology and it does not require the use of expensive equipment.…”

Section: Introductionmentioning

confidence: 99%

“…Liquid-liquid extraction using aqueous two-phase systems (ATPSs) has proved to be a promising separation strategy for many biological products [1][2][3][4][5]. To a large extent, interest in the partitioning behavior of proteins and other biomolecules in ATPSs have been stimulated by the potential of these systems to provide innocuous environments for the treatment of biological materials [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

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Salt effect on the (polyethylene glycol 8000+sodium sulfate) aqueous two-phase system: Relative hydrophobicity of the equilibrium phases

Ferreira

Teixeira

2011

The Journal of Chemical Thermodynamics

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Salt effect Relative hydrophobicity Gibbs free energy of transfer a b s t r a c tThe relative hydrophobicity of the phases of several {polyethylene glycol (PEG) 8000 + sodium sulfate (Na 2 SO 4 )} aqueous two-phase systems (ATPSs), all containing 0.01 mol Á L À1 sodium phosphate buffer (NaPB, pH 7.4) and increasing concentration of a salt additive, NaCl or KCl, up to 1.0 mol Á L À1 , was measured by the free energy of transfer of a methylene group between the phases, DG(CH 2 ). The DG(CH 2 ) of the systems was determined by partitioning of a homologous series of five sodium salts of dinitrophenylated (DNP) -amino acids with aliphatic side chains in three different tie-lines of each biphasic system. The relative hydrophobicity of the phases ranged from À0.125 to À0.183 kcal Á mol À1 , being the NaCl salt the one to provide the more effective changes. The results show that, within each system, there is a linear relationship between the DG(CH 2 ) and the tie-line length (TLL), and biphasic systems with high salt additive concentration present the most negative DG(CH 2 ) values. Therefore, the feasibility of establishing a relationship between the relative hydrophobicity of the phases in a given TLL and the ionic strength of the salt additive was investigated and a satisfactory correlation was found for each salt.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Salt effect on the (polyethylene glycol 8000+sodium sulfate) aqueous two-phase system: Relative hydrophobicity of the equilibrium phases

Ferreira

Teixeira

2011

The Journal of Chemical Thermodynamics

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“…These novel extraction systems have great potential in biological fields due to their biocompatibility [1,2]. The mutual competition between hydrophilic solvent and salt for water molecules leads to the exclusion of hydrophilic solvents or the crystallization of salts.…”

Section: Introductionmentioning

confidence: 99%

Liquid-liquid equilibrium of novel aqueous two-phase systems and evaluation of salting-out abilities of salts

Wang

Han

et al. 2010

Open Chemistry

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Binodal data are beneficial to the design of aqueous two-phase extraction and the establishment of thermodynamic models. For the 2-propanol + Li 2 SO 4 /Na 2 SO 4 / (NH 4 ) 2 SO 4 /K 3 PO 4 + water systems and 1-propanol + K 3 PO 4 /K 3 C 6 H 5 O 7 /(NH 4 ) 3 C 6 H 5 O 7 + water systems, binodal data were determined at 298.15 K. The binodal data were correlated by a theoretical equation on the basis of statistical geometry. The salting-out abilities of salts and the phase-separation abilities of alcohols were evaluated by the effective excluded volume of salt and the binodal curves plotted in molality. A simple Hofmeister series of cations and anions were obtained. The organic salt, K 3 C 6 H 5 O 7 , shows as high a salting-out ability as K 3 PO 4 .

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“…One type of liquid-liquid extraction is the aqueous two-phase micellar system (ATPMS), which has proved to be useful for extraction and purification of biomolecules such as proteins, viruses, DNA and antibiotics from unclarified microorganism cultures (Bordier, 1981;Albertsson, 1986;Kamei et al, 2002;Rangel-Yagui et al, 2003;Mazzola et al, 2008;Lopes et al, 2011;. Compared with other traditional purification techniques, ATPMS presents several advantages, such as high water content of both phases (80%, w/w) and biocompatibility, low interfacial tension (minimizing degradation of biomolecules), high capacity and yield, besides the possibility of surfactant recycling.…”

Section: Introductionmentioning

confidence: 99%

“…Compared with other traditional purification techniques, ATPMS presents several advantages, such as high water content of both phases (80%, w/w) and biocompatibility, low interfacial tension (minimizing degradation of biomolecules), high capacity and yield, besides the possibility of surfactant recycling. In addition, due to its simplicity, low cost and ease of scale-up, this process has been widely investigated for industrial applications (Mazzola et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Influence of salts on the coexistence curve and protein partitioning in nonionic aqueous two-phase micellar systems

Lopes

Santos-Ebinuma

Pessoa

et al. 2014

Braz. J. Chem. Eng.

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-Aqueous two-phase micellar systems (ATPMS) can be exploited in separation science for the extraction/purification of desired biomolecules. Prior to phase separation the surfactant solution reaches a cloud point temperature, which is influenced by the presence of electrolytes. In this work, we provide an investigation on the cloud point behavior of the nonionic surfactant C 10 E 4 in the presence of NaCl, Li 2 SO 4 and KI. We also investigated the salts' influence on a model protein partitioning. NaCl and Li 2 SO 4 promoted a depression of the cloud point. The order of salts and the concentration that decreased the cloud point was: Li 2 SO 4 0.5 M > NaCl 0.5 M ≈ Li 2 SO 4 0.2 M. On the other hand, 0.5 M KI dislocated the curve to higher cloud point values. For our model protein, glucose-6-phosphate dehydrogenase (G6PD), partitioning experiments with 0.5 M NaCl or 0.2 M Li 2 SO 4 at 13.85 °C showed similar results, with K G6PD ~ 0.46. The lowest partition coefficient was obtained in the presence of 0.5 M KI (K G6PD = 0.12), with major recovery of the enzyme in the micelle-dilute phase (%Recovery = 90%). Our results show that choosing the correct salt to add to ATPMS may be useful to attain the desired partitioning conditions at more extreme temperatures. Furthermore, this system can be effective to separate a target biomolecule from fermented broth contaminants.

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Liquid–liquid extraction of biomolecules: an overview and update of the main techniques

Cited by 225 publications

References 134 publications

Salt effect on the (polyethylene glycol 8000+sodium sulfate) aqueous two-phase system: Relative hydrophobicity of the equilibrium phases

Salt effect on the (polyethylene glycol 8000+sodium sulfate) aqueous two-phase system: Relative hydrophobicity of the equilibrium phases

Liquid-liquid equilibrium of novel aqueous two-phase systems and evaluation of salting-out abilities of salts

Influence of salts on the coexistence curve and protein partitioning in nonionic aqueous two-phase micellar systems

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