Liquid phase chromatography on open columns

Braithwaite, A.; Smith, Frederick M.

doi:10.1007/978-94-011-0599-6_4

Cited by 1 publication

(3 citation statements)

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“…In Adsorption chromatography sample component is separated according to its adsorption/desorption affinities on an active solid surface [5]. The Liquid/Gas is used as the movable phase and the polar substance which has a broad Specific surface area utilized as the stationary phase (adsorbent), different types of adsorbents as mention in Table 1 are used for chromatographic separation [3]. A solute binds with an adsorbent and makes a frail life 'bond' via "Vander Waals forces/electrostatic interaction/hydrogen bonds/hydrophobic interactions/dipole-dipole/dipole-induce dipole/л-complex bonding " on the outside of adsorbent as mention in Fig.…”

Section: Adsorption Chromatographymentioning

confidence: 99%

“…Open-Column Chromatography (LSC): Silica, Alumina, and cellulose are filled within a cylindrical glass tube; the size of the glass tube could be different in size depending on the number of components to be separated. Silica, Alumina, and cellulose are used as filling substances to prepare the column [3].It is a preparative application on a range from micrograms to kilograms of stationary phase. To prevent the loss of stationary phase different types of filters like glass wool plug/glass frit is used.…”

Section: ▪ Open Column Chromatography (Lsc) ▪ Tlc (Lsc) ▪ Hplc (Lsc) ...mentioning

confidence: 99%

“…1. The technique of M.S Tswett's was continued by scientist: Gottfried Kranzlin in Germany, Charles Dhere in Switzerland, and Leroy S. Palmer in the United States [1][2][3][4].…”

mentioning

confidence: 99%

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Chromatography: Introduction of Important Separation Methods and Modern Techniques for Adjustment of Column Parameters and Flow Rate in Liquid Chromatography to Reduce Analysis Time and Cost

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Chromatographic techniques play a major role in the pharmaceutical industries for separation, including clinical applications, environmental applications, forensics, food analysis, and the analysis of biological samples. Different chromatographic techniques are available for analysis, like TLC, HPLC, UPLC, GC, LCMS, ion exchange, affinity, and Size exclusion chromatography, etc. In each chromatographic technique, the movable (Mobile) phase and fixed (Stationary) phases play a major role in the separation of mixture compounds. In Liquid chromatography, the "small particle size" of the stationary phase increased the performance of chromatographic separation after adjustment in Column parameters (length & internal diameters), resulting in reduced analysis time, consumption of chemical/Solvent, and Cost of analysis which is support to Green Chemistry. Isolation of content depends on partitioning between mobile and stationary phases which is completed with the help of Vander Waals' forces through dipole-dipole, dipole-induce dipole, л-complex bonding, hydrogen bonding interaction, adsorption/desorption, Physico-chemical interaction, capillary ways holding-bonding pattern, size and shape pattern, and ligandreceptor phenomena. In all the above techniques the movable phase is liquid or gas whereas the stationary phase contains solid material and solid support coated with a liquid material.

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Section: Adsorption Chromatographymentioning

confidence: 99%

Section: ▪ Open Column Chromatography (Lsc) ▪ Tlc (Lsc) ▪ Hplc (Lsc) ...mentioning

confidence: 99%