Liquid-Phase Ion Trap for Ion Trapping, Transfer, and Sequential Ejection in Solutions

Hong, Jie; Hou, Chenyue; Xu, Zuqiang; He, Muyi; Xu, Wei

doi:10.1021/acs.analchem.0c01261

Cited by 6 publications

(8 citation statements)

References 71 publications

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“…The signal improvement is calculated using the maximum ion intensity during the square-wave-driven period divided by the ion intensity when no voltage was applied. With high enough voltage applied, ions during a square-wave cycle actually bunched together, which is similar to the case of a liquid-phase ion trap . Experiments were also carried out to demonstrate this effect, which could be found in Figure S2 in the Supporting Information.…”

Section: Resultsmentioning

confidence: 74%

“…With high enough voltage applied, ions during a square-wave cycle actually bunched together, which is similar to the case of a liquid-phase ion trap. 40 Experiments were also carried out to demonstrate this effect, which could be found in Figure S2 in the Supporting Information.…”

Section: ■ Results and Discussionmentioning

confidence: 96%

“…When we further increase this voltage (60 kV for instance), ions would even be concentrated at the entrance of the mobility region during this negative half cycle, similar to the case of a liquid-phase ion trap. 40 When the voltage polarity was switched to the positive half cycle, these concentrated ions were then released and pushed to the exit of the mobility region.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Ion motions in the liquid channel were simulated using a home-developed program, and details have been described in our previous publication. 40 Briefly, electric field force, viscous drag force, Laminar flow, and Taylor dispersion effects were considered and included. We used Phe as the simulated ions, where its hydrodynamic radius was set as 3.47 × 10 −10 m and the charge number as 0.2.…”

Section: ■ Instrumentation and Methodologymentioning

confidence: 99%

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Ion Bunching in Square-Wave-Driven Mobility Capillary Electrophoresis–Mass Spectrometry

Tan

Hong

2022

Anal. Chem.

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The ion-bunching effect was typically produced for ion beams in the gas phase, such as in ion accelerators. In this work, ion bunching was generated for ions in a liquid channel, specifically in a mobility capillary electrophoresis–mass spectrometry (MCE–MS) setup. MCE was recently developed and coupled with MS for ion separation and the precise measurements of ion hydrodynamic radius and effective charge in solution. In conventional MCE, a DC high voltage is applied, which serves as the separation voltage. In this study, square waves were employed to replace this DC voltage, and the ion-bunching phenomenon was observed and characterized in both simulations and experiments. After applying a high voltage square wave, cations and anions would be bunched and concentrated at the positive and negative half cycle of the square wave, respectively. Accordingly, ion signal intensities detected by the following mass spectrometer could be increased by up to ∼50 folds for the aspartic acid anion. This square wave could also dissociate metal adduct cations from nucleic acid anions, which results in stronger nucleic acid ion intensities (up to ∼10 folds) with cleaner backgrounds.

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Section: Resultsmentioning

confidence: 74%

Section: ■ Results and Discussionmentioning

confidence: 96%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Instrumentation and Methodologymentioning

confidence: 99%

See 2 more Smart Citations

Ion Bunching in Square-Wave-Driven Mobility Capillary Electrophoresis–Mass Spectrometry

Tan

Hong

2022

Anal. Chem.

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“…LPIT was proposed recently, which manipulates ions in solution using electric and liquid flow fields. Previous works have focused on target ion enrichments for improved detection sensitivity. − Different from IEF and CE, a liquid-phase ion trap (LPIT) separates ions based on their hydrodynamic radii and effective charges, and it could trap and separate ions in buffer solutions with volatile salts or even without salt.…”

Section: Introductionmentioning

confidence: 99%

Deep Bottom-up Proteomics Enabled by the Integration of Liquid-Phase Ion Trap

Hong

Zhai

et al. 2023

Anal. Chem.

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In bottom-up proteomics, the complexity of the proteome requires advanced peptide separation and/or fractionation methods to acquire an in-depth understanding of protein profiles. Proposed earlier as a solution-phase ion manipulation device, liquid phase ion traps (LPITs) were used in front of mass spectrometers to accumulate target ions for improved detection sensitivity. In this work, an LPIT-reversed phase liquid chromatography-tandem mass spectrometry (LPIT-RPLC-MS/MS) platform was established for deep bottom-up proteomics. LPIT was used here as a robust and effective method for peptide fractionation, which also shows good reproducibility and sensitivity on both qualitative and quantitative levels. LPIT separates peptides based on their effective charges and hydrodynamic radii, which is orthogonal to that of RPLC. With excellent orthogonality, the integration of LPIT with RPLC-MS/MS could effectively increase the number of peptides and proteins being detected. When HeLa cells were analyzed, peptide and protein coverages were increased by ∼89.2% and 50.3%, respectively. With high efficiency and low cost, this LPIT-based peptide fraction method could potentially be used in routine deep bottom-up proteomics.

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Electrokinetic Manipulations Combined With Direct and Ambient Ionization Mass Spectrometry

Manicke,

Wedasingha,

Rydberg

2024

Mass Spectrometry Reviews

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Mass spectrometry (MS) is a powerful analytical technique that typically involves sample preparation and online analytical separation before MS detection. Traditional methods often face bottlenecks in sample preparation and analytical separation, despite the rapid detection capabilities of MS. This review explores the integration of electrokinetic manipulations directly with the ionization step to enhance MS performance, focusing on methods that eliminate or simplify sample preparation and separation processes. Techniques such as paper spray, electrophoresis in nanoelectrospray ionization (nESI) emitters, induced nESI, counterflow gradient electrofocusing, and in‐syringe electrokinetics are highlighted for their ability to combine extraction and ionization in a single step, significantly improving throughput. The review delves into the use of electric fields during sample preparation and separations for these methods, demonstrating the efficiency of electrophoretic methods in driving extractions, crude separations, desalting, and enhanced sensitivity. The integration of these methods directly with MS ionization aims to enhance the analytical capabilities of mass spectrometry, while reducing costs and increasing throughput relative to traditional approaches.

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Liquid-Phase Ion Trap for Ion Trapping, Transfer, and Sequential Ejection in Solutions

Cited by 6 publications

References 71 publications

Ion Bunching in Square-Wave-Driven Mobility Capillary Electrophoresis–Mass Spectrometry

Ion Bunching in Square-Wave-Driven Mobility Capillary Electrophoresis–Mass Spectrometry

Deep Bottom-up Proteomics Enabled by the Integration of Liquid-Phase Ion Trap

Electrokinetic Manipulations Combined With Direct and Ambient Ionization Mass Spectrometry

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