Liquid storage of Ostrich (Struthio camelus) semen at 5 °C through intermediate dilution

Development of assays for the characterization of sperm motility parameters, viability, and membrane integrity in the epididymis and vas deferens of the greater rhea (Rhea americana)

“…An ideal hypoosmotic solution should apply sufficient osmotic stress to cause a visible increase in volume, while avoiding lysis of the sperm membrane ( Jeyendran et al, 1984 ). For the HOST test in ostriches, a hypoosmotic solution of 25 mOsm/l has been employed ( Smith et al, 2023 ), while emus have been evaluated using hypoosmotic solutions of 50 mOsm/L ( Matson et al, 2009 ). However, the assessment of sperm membrane functionality in rheas is currently lacking, and it is imperative to identify the optimal solution for conducting test of osmotic tolerance.…”

“…The evaluation of structural integrity of the sperm plasma membrane can be performed using vital dyes under optical microscopy, as demonstrated in emus ( Sood et al, 2012 ). Alternatively, contemporary methods highlight the effectiveness of fluorescent dye combinations in identifying membrane damage, as observed in ostriches ( Smith et al, 2023 ). In this regard, the utilization of propidium iodide (PI) and Hoechst 3342 in combination ( Bezerra et al, 2023b ) appears to be a feasible approach for analyzing the plasma membrane of rhea spermatozoa.…”