Liquid system scale up of<i>Carica papaya</i>L. somatic embryogenesis

Castillo, B.; Smith, M. Scott; Yadava, U. L.

doi:10.1080/14620316.1998.11510978

Cited by 12 publications

(11 citation statements)

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“…Ranch (1993) afirmou que a maturação em meio líquido facilitaria a pronta conversão de maior número de embriões somáticos. Castillo et al (1998) aumentaram a produtividade de embriões somáticos de Carica papaya L., bem como reduziram o tempo necessário para a maturação do embrião, cultivando calos embriogênicos em meio de maturação líquido.…”

Section: Resultsunclassified

DESENVOLVIMENTO DE CALOS EM EXPLANTES DE CUPUAÇUZEIRO (Theobroma grandiflorum Schum.) EM FUNÇÃO DA CONCENTRAÇÃO DE AUXINAS E DO MEIO LÍQUIDO

et al. 2001

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, ANDRÉA ALMEIDA CARNEIRO 5 e CARLOS FERREIRA DAMIÃO FILHO 6 RESUMO -Objetivou-se estudar o efeito da concentração de auxina e do meio líquido sobre o desenvolvimento de calos de cupuaçuzeiro. Segmentos de eixos embrionários e cotilédones, obtidos de frutos de cupuaçu dos tipos Mamorana e Redondo, foram cultivados em 4 meios de cultura diferentes: 1) meio MS (50%), suplementado com 2,4-D (1; 2; 4; 8 mg/L); 2) sais N6 (SIGMA) (4 g/L), acrescidos de 2,4-D (0; 2; 4 mg/L) e ANA (0; 3; 5 mg/L); 3) igual ao anterior, suplementado apenas com ANA (3 mg/L); e 4) meio MS, acrescido com ANA (1 mM). Calos com aspecto branco e brilhante foram observados em segmentos de eixos embrionários e cotilédones, cultivados nas menores concentrações de meio 1 (1 e 2 mg/L), enquanto nas maiores concentrações (4 e 8 mg/L) se observou a formação de calos e massa calosa branco-opaca, em eixos embrionários e em segmentos cotiledonares, estas estruturas tornaram-se escuras dentro de oito semanas. Usando o meio 2, um grande número de raízes foram formadas, enquanto o mesmo meio suplementado apenas com ANA (3; 5 mg/L) originou uma massa calosa. A combinação de ANA e 2,4-D, 3 e 2 mg/L, respectivamente, promoveu a formação de calos brancos e raízes. A transferência das culturas para meio líquido, sem regulador de crescimento, promoveu aumento de tamanho dos explantes e escurecimento dos mesmos. O cultivo desses explantes no meio 3 resultou no aparecimento de calos amarelos, com aspecto friável, que permaneceram com a mesma aparência no meio 4.Termos para indexação: cultura de tecidos, calos, auxina, Theobroma grandiflorum. DEVELOPMENT OF CUPUASSU CALLI (Theobroma grandiflorum Schum. ) IN FUNTION OF AUXIN CONCENTRATION AND LIQUID MEDIUMABSTRACT -It was studied the effect of auxin concentration and liquid medium on the development of cupuassu calli. Cupuassu embryonic axes segments and cotyledons, from kinds Mamorana and Redondo, were cultivated in four different culture media : 1) MS medium (50%) supplemented with 2,4-D (1; 2; 4; 8 mg/L); 2) N6 salts (SIGMA) (4 g/L) supplemented with 2,4-D (0; 2; 4 mg/L) and NAA (0; 3; 5 mg/L); 3) similar to the previous medium supplemented only with NAA (3 mg/L) and, 4) MS medium, supplemented with NAA (1 mM). Calli with white and bright aspect were observed in embryonic axes and cotyledons, cultivated in the smallest concentrations of medium 1 (1 and 2 mg/L), while in the largest concentrations (4 and 8 mg/L) opaque white calli and calli mass were formed in embryonic axes and in cotyledons segments, these structures became brownish within eight weeks. Using medium 2, a great number of roots were formed, while this same medium supplemented only with NAA (3; 5 mg/L) originated a calli mass. . The presence of NAA and 2,4-D, 3 and 2 mg/L respectively, promoted the formation of white calli and roots. The transfer of the explants to liquid medium, without growth regulator, promoted an increase of explants size and browning of them. The cultivation of those explants in medium 3 resulted in the formation of yellow...

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Section: Resultsunclassified

DESENVOLVIMENTO DE CALOS EM EXPLANTES DE CUPUAÇUZEIRO (Theobroma grandiflorum Schum.) EM FUNÇÃO DA CONCENTRAÇÃO DE AUXINAS E DO MEIO LÍQUIDO

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“…As reported by Monmarson et al (1995), Clarindo et al (2008) and Anandan et al (2012), zygotic embryos of papaya show high-embryogenic potential and quick response to tissue culture conditions. Comparatively, Fitch (1993) observed a callogenic response from hypocotyl segments between the 10th and 14th weeks, as did Castillo et al (1998a) around the sixth week.…”

Section: Somatic Embryogenesis and In Vitro Regenerationmentioning

confidence: 86%

“…This observation is consistent with the findings of Clarindo et al (2008) and Anandan et al (2012), who reported asynchronous maturation of C. papaya SE in a medium supplemented with ABA. ABA has been the most indicated PGR for regulating SE maturation in vitro (Chen and Chen 1992, Castillo et al 1998a, Anandan et al 2012. In some species, this PGR plays an important role in minimizing the secondary embryogenesis process, preventing early germination (von Arnold et al 2002) and stimulating deposition of reserve products (von Aderkas et al 2002).…”

Section: Somatic Embryogenesis and In Vitro Regenerationmentioning

confidence: 99%

“…Therefore, the results of the present study corroborate literature reports which show the liquid system to be effective for enhanced SE and plantlet production (Berthouly and Michaux-Ferriere 1996, Jordan and Velozo 1996, von Arnold et al 2002, Yang et al 2010, Anandan et al 2012, and thus very suitable for industrial scale. Castillo et al (1998a) and Anandan et al (2012) also compared the germination capacity of papaya SE produced in liquid and in agar-solidified media. Based on the higher yield per explant obtained in the first system in relation to the latter, the researchers concluded that the liquid system represents an appropriate strategy for mass proliferation, automation of rapid SE production, and continuous propagation of C. papaya elite lines.…”

Section: Somatic Embryogenesis and In Vitro Regenerationmentioning

confidence: 99%

“…The medium pH was adjusted to 5.7 prior to autoclaving. Petri dishes were sealed with a PVC film (Goodyear ® , Brazil), and cultures were kept in the dark at 27 C (Castillo et al 1998a, b, Clarindo et al 2008). …”

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Massal Induction of Carica papaya L. ^|^lsquo;Golden^|^rsquo; Somatic Embryos and Somaclone Screening by Flow Cytometry and Cytogenetic Analysis

2014

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Summary Somatic embryogenesis is a relevant micropropagation technique in Carica papayaGolden in view of the difficulties met in conventional seed propagation and the lack of an effective method for early sex determination in this trioecious species. Considering the interest in large-scale production of C. papaya seedlings, we adapted a somatic embryogenesis procedure associating liquid system and immature zygotic embryo explants. This protocol markedly contributed to an increase in the yield of friable embryogenic calli, resulting in a large number of normal somatic embryos at various developmental stages. From these embryos, plantlets were regenerated and evaluated for occurrence of somaclonal variation. Flow cytometry screening revealed diploid (88%), tetraploid (6%), and aneuploid (6%) plantlets. Owing to an interest in the mass propagation of diploid individuals, the karyotype of these plantlets was also analyzed. With this approach, diploidy (2X=18) was confirmed and no structural aberrations were found. Considering the results and the applicability of large-scale production of somatic embryos in C. papaya, the tissue culture protocols shown here could be used for commercial purposes. Moreover, the cytometric and cytogenetic analyses were crucial for a rapid, reliable and conclusive evaluation of the genetic stability of C. papaya plantlets regenerated by somatic embryogenesis.

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In Vitro Regeneration and Improvement in Tropical Fruit Trees: An Assessment

Singh

Jaiswal

Plant Biotechnology and Molecular Markers

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Liquid system scale up ofCarica papayaL. somatic embryogenesis

Cited by 12 publications

References 0 publications

DESENVOLVIMENTO DE CALOS EM EXPLANTES DE CUPUAÇUZEIRO (Theobroma grandiflorum Schum.) EM FUNÇÃO DA CONCENTRAÇÃO DE AUXINAS E DO MEIO LÍQUIDO

DESENVOLVIMENTO DE CALOS EM EXPLANTES DE CUPUAÇUZEIRO (Theobroma grandiflorum Schum.) EM FUNÇÃO DA CONCENTRAÇÃO DE AUXINAS E DO MEIO LÍQUIDO

Massal Induction of Carica papaya L. ^|^lsquo;Golden^|^rsquo; Somatic Embryos and Somaclone Screening by Flow Cytometry and Cytogenetic Analysis

In Vitro Regeneration and Improvement in Tropical Fruit Trees: An Assessment

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