Liriodenine inhibits dopamine biosynthesis and L-DOPA-induced dopamine content in PC12 cells

Jin, Chun Mei; Lee, Jae Joon; Yang, Yoo Jung; Kim, Yu Mi; Kim, Young Kyoon; Ryu, Shi Yong; Lee, Myung Koo

doi:10.1007/bf02993967

Cited by 14 publications

(16 citation statements)

References 38 publications

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“…Among them, aporphine isoquinoline alkaloids such as bulbocapnine, liriodenine, and anonaine show the inhibitory effects on dopamine biosynthesis by reducing the TH activity in PC12 cells [14,21]. Asimilobine, liriodenine, and anonaine have a similar molecular structure at the aporphine ring ( Figure 1).…”

Section: Discussionmentioning

confidence: 96%

“…liriodenine . bulbocapnine in comparison with their IC 50 values (anonaine, 0.05 mM; asimilobine, 0.13 mM; liriodenine, 8.4 mM; bulbocapnine 26.7 mM) [14,21]. Therefore, it is suggested that the isoquinoline ring planarity (liriodenine and anonaine/asimilobine; Figure 1) and the 11-hydroxy and methoxy groups of the aporphine alkaloid structure (bulbocapnine and liriodenine/anonaine/asimilobine) play a key role in the inhibition of dopamine biosynthesis in PC12 cells.…”

Section: Discussionmentioning

confidence: 99%

“…Aporphine isoquinoline alkaloids such as bulbocapnine, liriodenine, and anonaine show the inhibitory effects on dopamine biosynthesis in PC12 cells [14,21]. Bulbocapnine has antiperoxidative and dopamine D 1 receptor antagonistic effects [22].…”

Section: Introductionmentioning

confidence: 99%

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Effects of asimilobine on dopamine biosynthesis andl-DOPA-induced cytotoxicity in PC12 cells

Jin

Lee

Kim

et al. 2008

Journal of Asian Natural Products Research

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The effects of asimilobine, an aporphine isoquinoline alkaloid, on dopamine biosynthesis and L-DOPA-induced cytotoxicity in PC12 cells were investigated. Asimilobine at concentration ranges of 0.05-0.2 microM showed a significant inhibition of intracellular dopamine levels for 24 h in a concentration-dependent manner with an IC50 value of 0.13 microM. Asimilobine at 0.15 microM inhibited tyrosine hydroxylase (TH) and aromatic L-amino acid decarboxylase (AADC) activities at 24 h (73.2% inhibition of TH activity): the inhibition of TH activity was stronger and longer than that of AADC activity. Asimilobine also decreased TH mRNA levels and intracellular cyclic AMP levels, but not the basal Ca2+ concentrations. In addition, asimilobine at 0.05-5.0 microM, but not 10 microM, did not alter cell viability toward PC12 cells. A non-cytotoxic asimilobine (0.15 microM) associated with l-DOPA (20, 50, and 100 microM) for 24 h inhibited L-DOPA-induced increases in dopamine levels and enhanced L-DOPA-induced cell death when compared with L-DOPA alone. These results suggest that asimilobine inhibits dopamine biosynthesis by mainly reducing the TH activity and TH mRNA expression, and enhances L-DOPA-induced cytotoxicity in PC12 cells.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

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Effects of asimilobine on dopamine biosynthesis andl-DOPA-induced cytotoxicity in PC12 cells

Jin

Lee

Kim

et al. 2008

Journal of Asian Natural Products Research

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“…These results could account for the anti-depressive activity of the hexane extract of A. cherimolia. (JIN CM et al, 2007). FIGURE 1-Effects of acute administration of an alkaloid extract (TA) from A. cherimolia, imipramine, clomipramine, and fluoxetine on the FST.…”

Section: Annona Cherimolia As Source Of Potential Anti-depressive Agentsmentioning

confidence: 99%

Secondary metabolismin in Annonaceae: potencial source of drugs

Martínez‐Vázquez

Estrada-Reyes

2014

Rev. Bras. Frutic.

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-Several species of Annona (Annonaceae) are used in traditional Mexican medicine by their anti-anxiety, anticonvulsant and tranquilizing properties. It has been reported that the alkaloids isolated from some species of the Annona have affinity to serotonergic 5-HT1A receptors and modulate dopaminergic transmission, which is involved in depressive disorders. In this review it is showed the results of the antidepressant-like effect of an alkaloid extract from the aerial parts of Annona cherimola (TA) in mice. The antidepressant-like effect was evaluated in the forced swimming test. To elucidate a possible mechanism of action, experiments of synergism with antidepressant drugs, such as imipramine (IMI), clomipramine (CLIMI), and fluoxetine (FLX), were carried out. The neurotransmitter content (DA: dopamine, 5HT: serotonin and its metabolites, HVA: homovanillic acid and 5HIAA:5-hydroxyindoleacetic) in the whole brain of mice were also determined by HPLC method. The results showed that repeated treatment with TA produced antidepressant-like effects in mice. This effect was not related to an increase in locomotor activity. Administration of TA facilitated the antidepressant effect of IMI and CLIMI as well as increased the turnover of DA and 5-HT. The alkaloids: 1,2-dimethoxy-5, 6.6 to 7-tetrahydro-4H-dibenzoquinoline-3,8,9,10-tetraol, anonaine, liriodenine, and nornuciferine were the main constituents of TA. Index terms: antidepressant, alkaloids, Annona sp.. METABOLISMO SECUNDÁRIO EM ANNONACEA: FONTE POTENCIAL DE DROGASRESUMO -Várias espécies de Anona (Annonaceae) são utilizadas na medicina tradicional mexicana por seu antiansiedade anticonvulsiva e propriedades tranquilizantes. Tem sido relatado que os alcaloides isolados a partir de algumas espécies de Anona têm afinidade para receptores serotoninérgicos 5-HT1A e modular à transmissão dopaminérgica, que está envolvida em distúrbios depressivos.Nesta revisão, mostraram-se os resultados do efeito antidepressivo do extrato alcaloide das partes aéreas de Anona cherimólia (TA) em camundongos. O efeito antidepressivo foi avaliado no teste de natação forçada. Para elucidar um possível mecanismo de ação, as experiências de sinergismo com drogas antidepressivas, tais como a imipramina (IMI), clomipramina (CLIMI) e fluoxetina (FLX), foram realizadas. O teor de neurotransmissor (DA: serotonina e dos seus metabólitos, HVA:: dopamina, 5HT ácido homovanílico e 5HIAA :5-hidroxiindoleacético) em todo o cérebro de ratinhos foi também determinado pelo método de HPLC.Os resultados mostraram que o tratamento repetido com ácido tartárico produzido com antidepressivo provocou efeitos semelhantes em ratinhos. Este efeito não foi relacionado a um aumento da atividade locomotora. Administração da TA facilitou o efeito antidepressivo do IMI e CLIMI bem como o aumento do volume de negócios de DA e 5-HT. Os alcaloides de: 1,2-dimetoxi-5, 6,6 a, 7-tetrahydro-4H-dibenzoquinoline-3,8,9,10-tetraol, anonaine, liriodenina, e nornuciferine foram os principais constituintes da TA. Termos par...

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“…Furthermore, liriodenine has been shown to regulate dopamine biosynthesis by partially reducing tyrosine hydroxylase (TH) activity and TH gene expression and has protective effects against L-DOPA-induced cytotoxicity in PC12 cells (Jin et al, 2007). It is suggested that the isoquinoline ring planarity play a key role in the inhibition of dopamine.…”

Section: Central Nervous System Activitiesmentioning

confidence: 99%