Listeria monocytogenes isolated from vegetable salads sold at supermarkets in Santiago, Chile: Prevalence and strain characterization

Cordano, A. M.; Jacquet, Christine

doi:10.1016/j.ijfoodmicro.2009.04.008

Cited by 74 publications

(44 citation statements)

References 13 publications

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“…Using standard culture, L. monocytogenes was detected in almost 20.9% of these samples. Similar results were observed previously [18]where L. monocytogenes in very low counts was isolated from 25.4% of the samples of frozen vegetable salads. This observation shows that frozen vegetable products may be prone to L. monocytogenes contamination that may occur from the soil or irrigation water on fields, or during processing in a plant.…”

Section: Resultssupporting

confidence: 91%

Detection and Quantification of Listeria monocytogenes in Ready-to-eat Vegetables, Frozen Vegetables and Sprouts Examined by Culture Methods and Real-time PCR

Morávková¹,

Verbikova²,

Michna³

et al. 2017

JFNR

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In this study, a total of 175 samples of ready-to-eat vegetables, frozen vegetables and sprouted seeds originating in 10 states of the European Union and from 32 manufacturers were collected during a period of one year and examined for the presence of Listeria monocytogenes using standard culture methods and qPCR. In addition to these methods, isolation of Listeria monocytogenes was also carried out following a unified sample preparation for combined downstream use in culture and qPCR analysis. Standard culture and culture preceded by unified sample preparation, showed that L. monocytogenes was present in 6.9% and 11.4% of analyzed samples, respectively, in low numbers. Application of qPCR revealed only 2.3% of samples to be positive for L. monocytogenes in small quantities (less than 10 cells/gram). A statistically significant higher occurrence of L. monocytogenes was seen in frozen vegetables compared to ready to eat vegetables (p<0.01; Fisher's exact post-hoc tests with Bonferroni's correction) or sprouts (p<0.05; Fisher's exact post-hoc tests with Bonferroni's correction). Therefore, temperature abuse in food containing pieces of frozen vegetables without any processing such as cooking, may pose health risks, especially for sensitive individuals such as pregnant women, children, the elderly, and immunocompromised individuals.

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Section: Resultssupporting

confidence: 91%

Detection and Quantification of Listeria monocytogenes in Ready-to-eat Vegetables, Frozen Vegetables and Sprouts Examined by Culture Methods and Real-time PCR

Morávková¹,

Verbikova²,

Michna³

et al. 2017

JFNR

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show abstract

“…A number of studies have been carried out previously to detect the extent of microbiological contamination in fresh salad vegetables, but studies attempting to describe the survival of microorganisms in vegetables are limited [1,5,10,12,27]. Identifying the prevalence of microorganisms among the fresh-cut vegetables might aid in determining the ability of such foods to serve as substrates to enhance the growth of the spoilage microflora.…”

Section: Introductionmentioning

confidence: 99%

Determination of Microbial Growth and Survival in Salad Vegetables through in Vitro Challenge Test

Feroz¹,

Senjuti²,

Noor³

2013

IJNFS

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Current study attempted to examine the growth and subsequent survival of the common spoilage bacteria in vegetable samples collected from Dhaka, Bangladesh. Carrot, cucumber, tomato and lettuce samples were obtained from local markets and rendered free of contaminants. Each sample was then inoculated separately with an array of 9 test bacteria, resulting in the initial load of 10 5 cfu/g. The results revealed more than 6-log reduction of Salmonella spp. in carrot and tomato samples, Shigella spp. in carrot, lettuce and cucumber samples, Aeromonas spp. in tomato samples, Pseudomonas spp. in lettuce samples, and Listeria spp. in cucumber samples. No significant reduction in E. coli was observed in the cucumber samples, while in carrots and tomato samples, approximately 2-log reductions was found. Demonstration of the capacity of vegetables to influence microbial growth would further aid in the maintenance of the food quality and stability as well as their shelf life. Conducting such experiments after the quantification of spoiling microorganisms thus imparts a complete bacteriological profile, which is of public health significance.

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“…Thus, based on such assumptive logic and suggestive importance to examine the microbiological quality of apples and their ability to maintain or eliminate microorganisms in course of time, we estimated the longevity of the culturable microorganisms in the apple samples. In developing countries, this sort of research especially in case of apples is still in its infancy [28,44,45,46]. However, the results of our investigation clearly showed an average of 1 logreduction of microorganisms in all samples, with a relatively higher reduction frequency of Gala samples in case of the ebiquitous Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 52%

Microbiological Profiling of Imported Apples and Demonstration of Bacterial Survival Capacity through in vitro Challenge Test

Fatema¹,

Acharjee²,

Noor³

2013

AJMR

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Present study conducted a complete investigation on microorganisms contaminating the imported apples commonly consumed in Dhaka metropolis, Bangladesh. Study of microbial prevalence demonstrated a substantial number of total viable bacteria (~10 7 cfu/g) in each of the dissected portions, i. e., surface, body and core of the samples except for the body and core portion of Malus paulared (Paula Red). Among the specific pathogens, Staphylococcus spp. was found to be predominant within a range of 1.6×10 5 -2.8×10 7 cfu/g, while Escherichia coli, Klebsiella spp., Salmonella spp., Shigella spp., Pseudomonas spp., Vibrio spp. and Listeria spp. were also identified. Fungal contamination (~10 6 cfu/g) was observed only in the surface of each of the categories of apple samples tested. Study of antibiogram showed that most of the bacterial isolates were multi-drug resistant (MDR); however, study of antimicrobial activity showed that the Malus pumila (Golden delicious apple) samples possessed the anti-bacterial effect against Vibrio spp. Further studies employing the in vitro challenge test revealed a decline trend in the survival potential of spoiling bacteria in the apple samples tested.

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Listeria monocytogenes isolated from vegetable salads sold at supermarkets in Santiago, Chile: Prevalence and strain characterization

Cited by 74 publications

References 13 publications

Detection and Quantification of <i>Listeria</i><i> </i><i>m</i><i>onocytogenes</i> in Ready-to-eat Vegetables, Frozen Vegetables and Sprouts Examined by Culture Methods and Real-time PCR

Detection and Quantification of <i>Listeria</i><i> </i><i>m</i><i>onocytogenes</i> in Ready-to-eat Vegetables, Frozen Vegetables and Sprouts Examined by Culture Methods and Real-time PCR

Determination of Microbial Growth and Survival in Salad Vegetables through in Vitro Challenge Test

Microbiological Profiling of Imported Apples and Demonstration of Bacterial Survival Capacity through <i>in vitro</i> Challenge Test

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