Listeria-vectored multi-antigenic tuberculosis vaccine protects C57BL/6 and BALB/c mice and guinea pigs against Mycobacterium tuberculosis challenge

Jia, Qingmei; Masleša-Galić, Saša; Nava, Susana; Horwitz, Marcus A.

doi:10.1038/s42003-022-04345-1

Cited by 2 publications

(1 citation statement)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The safety and immunogenicity of rLmMtb9Ag were evaluated in mice and guinea pigs without primary vaccination using BCG. This multi-antigen vaccine induced the proliferation of antigen-specific CD4 + T cells and CD8 + T cells, reduced the CFU count of Mtb in the lungs and spleen, and produced protective immunity in guinea pigs infected with an aerosol of the hypervirulent Erdman strain of Mtb ( 145 ). The high immunogenicity of PPE68 may be due to its P9 peptide composed of amino acids 121–145, which is highly conserved in pathogenic mycobacteria ( 146 ).…”

Section: Novel Vaccines Based On Pe/ppe Family Proteins Against Tbmentioning

confidence: 99%

Immunological effects of the PE/PPE family proteins of Mycobacterium tuberculosis and related vaccines

Guo,

Wei,

Song

et al. 2023

Front. Immunol.

View full text Add to dashboard Cite

Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis (Mtb), and its incidence and mortality are increasing. The BCG vaccine was developed in the early 20th century. As the most widely administered vaccine in the world, approximately 100 million newborns are vaccinated with BCG every year, which has saved tens of millions of lives. However, due to differences in region and race, the average protective rate of BCG in preventing tuberculosis in children is still not high in some areas. Moreover, because the immune memory induced by BCG will weaken with the increase of age, it is slightly inferior in preventing adult tuberculosis, and BCG revaccination cannot reduce the incidence of tuberculosis again. Research on the mechanism of Mtb and the development of new vaccines against TB are the main strategies for preventing and treating TB. In recent years, Pro-Glu motif-containing (PE) and Pro-Pro-Glu motif-containing (PPE) family proteins have been found to have an increasingly important role in the pathogenesis and chronic protracted infection observed in TB. The development and clinical trials of vaccines based on Mtb antigens are in progress. Herein, we review the immunological effects of PE/PPE proteins and the development of common PE/PPE vaccines.

show abstract

Section: Novel Vaccines Based On Pe/ppe Family Proteins Against Tbmentioning

confidence: 99%

Immunological effects of the PE/PPE family proteins of Mycobacterium tuberculosis and related vaccines

Guo,

Wei,

Song

et al. 2023

Front. Immunol.

View full text Add to dashboard Cite

show abstract

Mycobacterium tuberculosis strain with deletions in menT3 and menT4 is attenuated and confers protection in mice and guinea pigs

Gosain,

Chugh,

Rizvi

et al. 2024

Nat Commun

View full text Add to dashboard Cite

The genome of Mycobacterium tuberculosis encodes for a large repertoire of toxin-antitoxin systems. In the present study, MenT3 and MenT4 toxins belonging to MenAT subfamily of TA systems have been functionally characterized. We demonstrate that ectopic expression of these toxins inhibits bacterial growth and this is rescued upon co-expression of their cognate antitoxins. Here, we show that simultaneous deletion of menT3 and menT4 results in enhanced susceptibility of M. tuberculosis upon exposure to oxidative stress and attenuated growth in guinea pigs and mice. We observed reduced expression of transcripts encoding for proteins that are essential or required for intracellular growth in mid-log phase cultures of ΔmenT4ΔT3 compared to parental strain. Further, the transcript levels of proteins involved in efficient bacterial clearance were increased in lung tissues of ΔmenT4ΔT3 infected mice relative to parental strain infected mice. We show that immunization of mice and guinea pigs with ΔmenT4ΔT3 confers significant protection against M. tuberculosis infection. Remarkably, immunization of mice with ΔmenT4ΔT3 results in increased antigen-specific TH1 bias and activated memory T cell response. We conclude that MenT3 and MenT4 are important for M. tuberculosis pathogenicity and strains lacking menT3 and menT4 have the potential to be explored further as vaccine candidates.

show abstract

Listeria-vectored multi-antigenic tuberculosis vaccine protects C57BL/6 and BALB/c mice and guinea pigs against Mycobacterium tuberculosis challenge

Cited by 2 publications

References 36 publications

Immunological effects of the PE/PPE family proteins of Mycobacterium tuberculosis and related vaccines

Immunological effects of the PE/PPE family proteins of Mycobacterium tuberculosis and related vaccines

Mycobacterium tuberculosis strain with deletions in menT3 and menT4 is attenuated and confers protection in mice and guinea pigs

Contact Info

Product

Resources

About