2009
DOI: 10.1152/ajpgi.00040.2009
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Listeriolysin O affects barrier function and induces chloride secretion in HT-29/B6 colon epithelial cells

Abstract: Listeria monocytogenes is a food-borne pathogen, which is able to induce diarrhea when residing in the intestine. We studied the effect of listeriolysin O (LLO), an extracellular virulence factor of L. monocytogenes, on intestinal transport and barrier function in monolayers of HT-29/B6 human colon cells using the Ussing technique to understand the pathomechanisms involved. Mucosal addition of LLO, but not a LLO mutant, induced a dose-and pH-dependent increase in short-circuit current (I SC). Sodium and chlori… Show more

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Cited by 22 publications
(19 citation statements)
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“…L. monocytogenes infection is known to reduce the TEER of epithelial monolayers (33), as also observed in this study (Table 2), which can contribute to paracellular bacterial translocation (15,27). Although the exact mechanism for a Listeriainduced reduction in TEER is unknown, it has been linked to virulence traits such as actin cytoskeleton disruption following invasion (26,52,60), as well as extracellular secretion of LLO (50). Although the presence or absence of LAP expression did not induce changes in TEER (Table 2), it is possible that a weakened epithelial barrier facilitates LAP-Hsp60-mediated bacterial translocation.…”
supporting
confidence: 61%
“…L. monocytogenes infection is known to reduce the TEER of epithelial monolayers (33), as also observed in this study (Table 2), which can contribute to paracellular bacterial translocation (15,27). Although the exact mechanism for a Listeriainduced reduction in TEER is unknown, it has been linked to virulence traits such as actin cytoskeleton disruption following invasion (26,52,60), as well as extracellular secretion of LLO (50). Although the presence or absence of LAP expression did not induce changes in TEER (Table 2), it is possible that a weakened epithelial barrier facilitates LAP-Hsp60-mediated bacterial translocation.…”
supporting
confidence: 61%
“…Other deleterious effects of increased Ca 2 þ levels observed at early stages of Listeria infection include loss of cell volume, change of cell size and a weakening of the epithelial monolayer 40 .…”
Section: Resultsmentioning
confidence: 99%
“…LLO induces a loss of TER and an increase in transepithelial flux in fully differentiated HT-29/B6 cells (552).…”
Section: Structural and Functional Injuries At The Junctional Domainmentioning
confidence: 93%
“…LLO induces an increase in short-circuit current and chloride secretion in fully differentiated HT-29/B6 cells (552).…”
Section: Structural and Functional Injuriesmentioning
confidence: 93%