Listeriose em rebanho de ovinos leiteiros na região serrana do Rio Grande do Sul: relato de caso

Ribeiro, Luiz Alberto Oliveira; Rodrigues, Norma Centeno; Fallavena, Luiz Cesar Bello; Oliveira, Sérgio José de; Brito, Marcelo Arnt

doi:10.1590/s0102-09352006000300005

Cited by 10 publications

(14 citation statements)

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“…This is partially because ruminant listeriosis in Brazil is not of significant economic importance to the national herd considering the reduced levels of occurrence described in cattle, sheep, and goats (Sanches et al , 2000; Guedes et al , 2007; Galiza et al , 2010), and the marked absence of silage and hay feeding of ruminants relative to temperate zones, which makes ruminants in Brazil less susceptible to infection by L. monocytogenes (Headley et al , 2013). Hence, the objective of the current study was to confirm the participation of L. monocytogenes by molecular technique, since there are no reports of small ruminant listeriosis within the state in which these animals were housed, and few descriptions of small ruminant listeriosis in Brazil (Schwab et al , 2004; Ribeiro et al , 2006; Rissi et al , 2006; Guedes et al , 2007; Rissi et al , 2010). Additionally, the hly gene is highly conserved in L. monocytogenes , the primer pairs (Lis1; Lis2) are specie-specific, and differentiates L. monocytogenes from other species of Listeria (Deneer and Boychuk 1991); this further confirms that L. monocytogenes was involved in the brainstem lesions of these animals.…”

Section: Discussionmentioning

confidence: 98%

“…Although this bacterium occurs worldwide, disease in domestic animals is more frequently diagnosed in temperate countries (George 2009). In Brazil, there are few descriptions of listeriosis in domestic animals, with disease occurring in cattle (Schwab et al , 2004; Galiza et al , 2010), sheep (Schwab et al , 2004; Ribeiro et al , 2006; Rissi et al , 2010), and goats (Schwab et al , 2004; Rissi et al , 2006). Data collected from diagnostic laboratories in Brazil have suggested that the occurrence of ruminant encephalitic listeriosis (REL) is significantly reduced (Sanches et al , 2000; Guedes et al , 2007; Galiza et al , 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Confirmation of REL has been based on the combination of these characteristic histopathological findings associated with immuno-histochemistry (Wilkins et al , 2000; Schwab et al , 2004; Ribeiro et al , 2006; Rissi et al , 2006), bacterial isolation (Yousif et al , 1984; McLaughlin et al , 1993; Johnson et al , 1995), serology (Börkü et al , 2006), and molecular biology (Wiedmann et al , 1997; Evans et al , 2004; Langohr et al , 2006; Bundrant et al , 2011). …”

Section: Introductionmentioning

confidence: 99%

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Histopathological and molecular characterization of encephalitic listeriosis in small ruminants from northern Paraná, Brazil

Headley

Bodnar

Fritzen

et al. 2013

Braz. J. Microbiol.

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Listeriosis is a disease primarily of ruminants caused by the Gram-positive bacterium Listeria monocytogenes. Ruminants either demonstrate manifestations of the encephalitic, septicemic, or reproductive form of listeriosis. The pathological and molecular findings with encephalitic listeriosis in a 5.5-month-old, male, mixed-breed goat and a 3-year-old Texel-crossed sheep from northern Paraná, Brazil are described. Clinically, the kid demonstrated circling, lateral protrusion of the tongue, head tilt, and convulsions; the ewe presented ataxia, motor incoordination, and lateral decumbency. Brainstem dysfunctions were diagnosed clinically and listeriosis was suspected. Necropsy performed on both animals did not reveal remarkable gross lesions; significant histopathological alterations were restricted to the brainstem (medulla oblongata; rhombencephalitis) and were characterized as meningoencephalitis that consisted of extensive mononuclear perivascular cuffings, neutrophilic and macrophagic microabscesses, and neuroparenchymal necrosis. PCR assay and direct sequencing, using genomic bacterial DNA derived from the brainstem of both animals, amplified the desired 174 base pairs length amplicon of the listeriolysin O gene of L. monocytogenes. Phylogenetic analyses demonstrated that the strains associated with rhombencephalitis during this study clustered with known strains of L. monocytogenes lineage I from diverse geographical locations and from cattle of the state of Paraná with encephalitic listeriosis. Consequently, these strains should be classified as L. monocytogenes lineage I. These results confirm the active participation of lineage I strains of L. monocytogenes in the etiopathogenesis of the brainstem dysfunctions observed during this study, probably represent the first characterization of small ruminant listeriosis by molecular techniques in Latin America, and suggest that ruminants within the state of Paraná were infected by the strains of the same lineage of L. monocytogenes.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Histopathological and molecular characterization of encephalitic listeriosis in small ruminants from northern Paraná, Brazil

Headley

Bodnar

Fritzen

et al. 2013

Braz. J. Microbiol.

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“…Essa doença apresenta baixos índices de morbidade e alta letalidade e nesses casos a infecção geralmente é associada à alimentação com silagem ou contato com fezes ou leite de animais portadores (Vandegraaff et al 1981, Wilesmith & Gitter 1986, George 2002, Rissi et al 2006, Schild 2007. A forma neurológica de listeriose em ruminantes no Brasil já foi descrita em bovinos (Sanches et al 2000), caprinos (Rissi et al 2006, Guedes et al 2007) e ovinos (Mendes et al 2005, Ribeiro et al 2006, Guedes et al 2007), porém não existem descrições detalhadas da forma neurológica da enfermidade em ovinos.…”

Section: Introductionunclassified

Meningoencefalite por Listeria monocytogenes em ovinos

et al. 2010

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RESUMO.-São descritos sete casos de doença neuroló-gica em ovinos por Listeria monocytogenes no Rio Grande do Sul e Paraná entre 2000 e 2007. Foram afetados ovinos com idades entre 12-24 meses. Os casos ocorreram no verão e início da primavera e os índices gerais de morbidade e letalidade foram de 3,15% e 100%, respectivamente. Quando essa informação estava disponível, nenhum dos ovinos afetados era alimentado com silagem. Em três propriedades havia contato próximo dos ovinos afetados com outras espécies. A evolução do quadro clí-nico foi de 12 horas a três dias e os sinais clínicos foram caracterizados por decúbito (7/7), desvio da cabeça (4/ 7), incoordenação (3/7), depressão (3/7), andar em círcu-los (2/7), cegueira unilateral, emagrecimento progressivo, febre, midríase, movimentos de pedalagem, nistagmo lateral, opistótono, paralisia flácida dos membros pélvi-cos ou dos quatro membros, salivação excessiva e tremores (1/7 cada). Histologicamente observou-se encefalite com microabscessos, predominantemente unilateral com variáveis graus de gliose e alterações degenerativas como esferóides axonais e infiltração de células Gitter. As lesões se estendiam desde a medula oblonga até o mesencéfalo. Overall morbidity and lethality rates were 3.15% and 100%, respectively. Cases occurred in the summer and early spring. When this information was available, affected sheep had not been fed with silage. In three farms there were close contact among affected sheep and other species. Clinical signs were characterized by recumbency (7/7), head tilt (4/7), incoordination (3/7), depression (3/7), circling (2/7), unilateral blindness, wasting, fever, midriasis, paddling, opisthotonus, hind or hind and fore limb paralysis, drooling, and muscle tremors (1/7 each). Clinical evolution varied from 12 hours to three days. Histological findings consisted of predominantly unilateral, microabscedative encephalitis with variable degrees of gliosis and degenerative lesions characterized by axonal spheroids and infiltration by Gitter cells. These lesions were observed extending from medulla oblongata to mesencephalon. Listeria monocytogenes antigen was showed by imunohistochemistry in routinely processed sections of brainstem from all seven affected sheep. The diagnostic was based on epidemiological, clinical, and pathological findings and confirmed by immunohistochemistry (IHQ) using polyclonal anti-L. monocytogenes antibody.

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“…Regarding Listeria, the presence of this microorganism indicates an environmental contamination (SCHOCKEN-ITURRINO et al, 2005;RIBEIRO et al, 2006), which is very frequent in silages. Of the 10 samples evaluated at 30 days of maturation, three were positive for Listeria.…”

Section: Resultsmentioning

confidence: 99%

Microbiota indigenous milk, mesophilic lipolytic and proteolytic colonial cheese matured, produced at different times of the year

Pereira

Pozza

Olivo

et al. 2017

Rev. bras. saúde prod. anim.

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Listeriose em rebanho de ovinos leiteiros na região serrana do Rio Grande do Sul: relato de caso

Cited by 10 publications

References 1 publication

Histopathological and molecular characterization of encephalitic listeriosis in small ruminants from northern Paraná, Brazil

Histopathological and molecular characterization of encephalitic listeriosis in small ruminants from northern Paraná, Brazil

Meningoencefalite por Listeria monocytogenes em ovinos

Microbiota indigenous milk, mesophilic lipolytic and proteolytic colonial cheese matured, produced at different times of the year

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