Lithotriptic and Anti-Lithogenic Effect of Tila-Dhatri Kshara Along With Gokshura Kashaya in Ethylene Glycol-Induced Nephrolithiasis in Adult Male Charles Foster Rats

Abstract

Objectives: Thirty-five adult Charles Foster rats were taken in the study to assess the lithotriptic and anti-lithogenic activity of the Trial drug. Methods: Thirty-five were divided into seven groups of five animals each. One group was kept as normal control; one group was given ethylene glycol 0.75% alone for 28 days. In the next three groups, Trial drug, kshara with distilled water, and the Standard drug were given, respectively, for 28 days after induction of calculi using ethylene glycol. In the next two groups, to assess the anti-lithogenic activity, simultaneously stone inducing agents and trial/standard drugs were given for 28 days. At the end, blood, urine, and histopathology of rat kidneys were done. Results: Tila-Dhatri kshara with Gokshura Kashaya and standard drug, Potassium Magnesium Citrate B6 showed equal lithotriptic and anti-lithogenic activity as depicted from blood, urine biochemistry, urine microscopy, and histopathology of rat kidneys. Trial drug-treated group showed more diuresis than other groups. Furthermore, serum LDH values were significantly reduced in trial drug-treated group. Conclusion: The trial drug showed significant nephroprotective property when compared to the standard drug as depicted from the serum LDH values and histopathological evaluations.