Litopenaeus vannamei attenuates white spot syndrome virus replication by specific antiviral peptides generated from hemocyanin

Zhan, Shixiong; Aweya, Jude Juventus; Wang, Fan; Yao, Defu; Zhong, Min; Chen, Jiehui; Li, Shengkang; Zhang, Yueling

doi:10.1016/j.dci.2018.10.005

Cited by 49 publications

(27 citation statements)

References 59 publications

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“…Most of the known AMPs come from the processing of larger inactive proteins; however, some studies suggest that biologically active proteins, such as hemocyanin [ 18 ] and hemoglobin [ 33 ], can be sources of AMPs. Hemocyanin-derived peptides with antimicrobial properties have previously been reported in shrimp [ 34 , 35 , 36 ], crayfish [ 18 ] and spiders [ 16 ]. The bibliographic review indicates that until 2015, the first AMPs derived from hemocyanin from mollusks were registered [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial, Antibiofilm and Anti-Virulence Activity of Biactive Fractions from Mucus Secretion of Giant African Snail Achatina fulica against Staphylococcus aureus Strains

et al. 2021

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Staphylococcus aureus is an important etiological agent that causes skin infections, and has the propensity to form biofilms, leading to significant mortality and morbidity in patients with wounds. Mucus secretion from the Giant African snail Achatina fulica is a potential source of biologically active substances that might be an important source for new drugs to treat resistant and biofilm-forming bacteria such as S. aureus. This study evaluated the effect of semi-purified fractions from the mucus secretion of A. fulica on the growth, biofilm formation and virulence factors of S. aureus. Two fractions: FMA30 (Mw >30 kDa) and FME30 (Mw 30−10 kDa) exhibited antimicrobial activity against S. aureus with a MIC50 of 25 and 125 µg/mL, respectively. An inhibition of biofilm formation higher than 80% was observed at 9 µg/mL with FMA30 and 120 µg/mL with FME30. Furthermore, inhibition of hemolytic and protease activity was determined using a concentration of MIC20, and FME30 showed a strong inhibitory effect in the formation of clots. We report for the first time the effect of semi-purified fractions of mucus secretion of A. fulica on biofilm formation and activity of virulence factors such as α-hemolysin, coagulase and proteases produced by S. aureus strains.

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Section: Discussionmentioning

confidence: 99%

Antibacterial, Antibiofilm and Anti-Virulence Activity of Biactive Fractions from Mucus Secretion of Giant African Snail Achatina fulica against Staphylococcus aureus Strains

et al. 2021

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“…These results were confirmed by in vivo experiments, demonstrating that WSSV pre-treated viruses with LvHcL48 peptides decreased the wsv069/wsv421 mRNA levels at 6, 12, and 24 hpi compared to the control group (PBS + WSSV). In addition, Far-Western blotting assay on WSSV lysates demonstrated that LvHcL48 was able to interact to the viral envelope protein VP28 [183].…”

Section: Crustaceansmentioning

confidence: 98%

Ten-Year Research Update Review: Antiviral Activities from Marine Organisms

et al. 2020

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Oceans cover more than 70 percent of the surface of our planet and are characterized by huge taxonomic and chemical diversity of marine organisms. Several studies have shown that marine organisms produce a variety of compounds, derived from primary or secondary metabolism, which may have antiviral activities. In particular, certain marine metabolites are active towards a plethora of viruses. Multiple mechanisms of action have been found, as well as different targets. This review gives an overview of the marine-derived compounds discovered in the last 10 years. Even if marine organisms produce a wide variety of different compounds, there is only one compound available on the market, Ara-A, and only another one is in phase I clinical trials, named Griffithsin. The recent pandemic emergency caused by SARS-CoV-2, also known as COVID-19, highlights the need to further invest in this field, in order to shed light on marine compound potentiality and discover new drugs from the sea.

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“…Proteolytic cleavage of haemocyanin by proteases (Lee et al 2003;Li et al 2018a) can generate various peptides with antimicrobial activity constitutively (Wen et al 2016), but the levels of these antimicrobial peptides increase significantly during injury or upon immune challenge (Coates & Decker 2017;Zhan et al 2019). Thus, haemocyanin is considered the precursor (Lee et al 2004) or source of broad-spectrum AMPs generated during infections (see recent review by (Coates & Costa-Paiva 2020)), which is akin to AMPs produced from haemoglobin in vertebrates (Sheshadri & Abraham 2012).…”

Section: Haemocyaninmentioning

confidence: 99%

The expanding repertoire of immune‐related molecules with antimicrobial activity in penaeid shrimps: a review

Aweya

Zheng

et al. 2021

Reviews in Aquaculture

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Shrimps live in a complex aquatic ecosystem with numerous other biotic and abiotic factors, especially pathogenic microorganisms, which require the adaptation of prudent protective and survival strategies. As invertebrates, shrimps reply on humoral and cellular innate immune response mechanisms for protection against pathogens. One integral part of the humoral innate immune defence mechanism involves many biomolecules that are constitutively expressed or induced by pathogens. Shrimps, therefore, produce various biomolecules and secondary metabolites for physiological and pathophysiological purposes, most of which also act as effector molecules in immune-related functions. A growing number of molecules have been identified as essential constituents of the immune arsenal of biomolecules in penaeid shrimps. This review provides a synthesis of important information on penaeid shrimp-encoded immune-related molecules with antimicrobial activities, their molecular regulation and importance in shrimp innate immune response. Research gaps would be identified and proposed research themes emphasized for future studies.

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Litopenaeus vannamei attenuates white spot syndrome virus replication by specific antiviral peptides generated from hemocyanin

Cited by 49 publications

References 59 publications

Antibacterial, Antibiofilm and Anti-Virulence Activity of Biactive Fractions from Mucus Secretion of Giant African Snail Achatina fulica against Staphylococcus aureus Strains

Antibacterial, Antibiofilm and Anti-Virulence Activity of Biactive Fractions from Mucus Secretion of Giant African Snail Achatina fulica against Staphylococcus aureus Strains

Ten-Year Research Update Review: Antiviral Activities from Marine Organisms

The expanding repertoire of immune‐related molecules with antimicrobial activity in penaeid shrimps: a review

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