Litopenaeus vannamei heat shock protein 70 (LvHSP70) enhances resistance to a strain of Vibrio parahaemolyticus, which can cause acute hepatopancreatic necrosis disease (AHPND), by activating shrimp immunity

Junprung, Wisarut; Supungul, Premruethai; Tassanakajon, Anchalee

doi:10.1016/j.dci.2018.09.011

Cited by 34 publications

(9 citation statements)

References 30 publications

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“…The recombinant HSP70 was successfully overexpressed in vivo to clarify the function of the HSP70 gene expressed in N. barkeri in heat tolerance. In Pacific white shrimp Litopenaeus vannamei , recombinant LvHSP70 in E. coli could enhance the capability of the bacterial defense by inducing the immune system 45 . Significantly, the recombinant expression of the N‐terminal domain of hyperthermophilic L‐asparaginase in HSPs from archaeon Pyrococcus furiosus guaranteed E. coli to maintain typical growth behavior at 52 °C and even withstand an extreme heat shock up to 62 °C 46 .…”

Section: Discussionmentioning

confidence: 99%

Sustaining induced heat shock protein 70 confers biological thermotolerance in a high‐temperature adapted predatory mite Neoseiulus barkeri (Hughes)

Tian

et al. 2020

Pest Management Science

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BACKGROUND In fluctuating climatic environments, heat acclimation in predatory mites is a superior adaptation strategy for effective agricultural pest management and can be used to enhance the abilities critical in biological control efficiency. We investigated the regulatory mechanism governing the remarkable plastic response of thermotolerance in a high‐temperature adapted strain (HTAS) and discerned the differences in the defensive reactions between the HTAS and the conventional strain (CS) in the predatory mite Neoseiulus barkeri. RESULTS At 42 °C, the relative expression levels of four identified HSP70 genes increased rapidly in both N. barkeri strains; meanwhile the expression of NbHSP70‐1 and NbHSP70‐2 in CS sharply decreased after 4 h, displaying a distinct contrast with the remaining elevated expression in HTAS. Western blot analysis showed that the protein level of NbHSP70‐1 in CS was dramatically elevated at 0.5 h and decreased at 6 h at 42 °C. Conversely, in HTAS, NbHSP70‐1 was constantly induced and peaked at 6 h at 42 °C. Furthermore, HSP70 suppression by RNAi knockdown had a greater influence on the survival of HTAS, causing a higher mortality under high temperature than CS. Finally, the recombinant exogenous NbHSP70‐1 protein enhanced the viability of E. coli BL21 under a lethal temperature of 50 °C. CONCLUSION Sustained accumulation of HSP70 proteins results in predatory phytoseiid mites with the thermotolerance advantage that could promote their biological control function to pests. The divergent constitutive regulation of HSP70 to a thermal environment is conducive to the flexible adaptability of predators in the higher trophic level to trade off under extremely adversity stress.

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Section: Discussionmentioning

confidence: 99%

Sustaining induced heat shock protein 70 confers biological thermotolerance in a high‐temperature adapted predatory mite Neoseiulus barkeri (Hughes)

Tian

et al. 2020

Pest Management Science

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show abstract

“…In Litopenaeus vannamei , recombinantLvHSP70 inE. coli could enhance the capability in bacterial defense by inducing the immune system (Junprung et al, 2019). Significantly, the recombinant expression of N-terminal domain of hyperthermophilic L-asparaginase in HSPs from Pyrococcus furiosus guaranteed E. coli to maintain typical growth behavior at 52degC and even withstand an extreme heat shock up to 62degC (Jena et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Sustaining induced heat shock protein 70 confers biological thermotolerance: a case in a high-temperature adapted predatory mite

Tian¹,

Li²,

Wu³

et al. 2020

Preprint

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Tiny predators, especially like phytoseiid mites, often experience a host of threats or stresses by fluctuating environmental factors. Heat acclimation as a superior adaptation strategy critically enhances abilities for organisms to handle with changing climate, but little is known about the molecular mechanism determining tolerant plastic responses in Phytoseiid mites. The relative expression of four identified HSP70 genes in two strains of Neoseiulus barkeri increased within a short time in temperature ramping treatment; meanwhile the expression of NbHSP70-1 and NbHSP70-2 in the conventional strain (CS) sharply decreased after 4 h displaying distinct contrast with the stable expression in the high-temperature adapted strain (HTAS). Western blot analysis showed that the protein level of NbHSP70-1 in CS was dramatically elevated at 0.5 h and decreased at 6 h at 42°C. Conversely, in HTAS, NbHSP70-1 was constantly induced and peaked at 6 h changed at 42°C. Furthermore, HSP70 suppression by RNAi knockdown had a greater influence on the survival of HTAS, causing a higher mortality under high temperature than CS. The recombinant certain exogenous NbHSP70-1 protein enhanced the viability of E. coli BL21 under lethal temperature of 50°C. These results suggested that HSP70 genes were a prominent contributor promoting the thermotolerance to heat stress and plastic change of HSP70 genes conferred the thermotolerance of HTAS through long-term heat acclimation. The divergent constitutive regulation of HSP70 to thermal is conducive to the flexible adaptability of predators in higher trophic level to trade off under extremely adversity stress.

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“…HSP70 acts as a molecular chaperone and stress protein, but it also plays an important role in innate immune responses. In Litopenaeus vannamei (Pacific white shrimp), the injection of recombinant HSP70 protein induced the upregulation of several immune-related genes involved in the NF-κB signal transduction pathway, AMP synthesis, and ProPO activating system, and the shrimp receiving injection showed higher resistance to pathogenic Vibrio parahaemolyticus [47]. Serpin expression in M. japonicus (MjSerp1) was upregulated by Vibrio anguillarum infection and inhibited microbial serine protease [48].…”

Section: Upregulation Of the Innate Immunity Genes By Pnsb Treatmentmentioning

confidence: 99%

Probiotic Effects of a Marine Purple Non-Sulfur Bacterium, Rhodovulum sulfidophilum KKMI01, on Kuruma Shrimp (Marsupenaeus japonicus)

et al. 2022

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Purple non-sulfur bacteria (PNSB) are used as probiotics in shrimp aquaculture; however, no studies have examined the probiotic effects of PNSB in shrimp at the gene expression level. In this study, we examined the effects of a marine PNSB, Rhodovulum sulfidophilum KKMI01, on the gene expression of kuruma shrimp (Marsupenaeus japonicus). Short-term (3 days) effects of R. sulfidophilum KKMI01 on the gene expression in shrimp were examined using small-scale laboratory aquaria experiments, while long-term (145 days) effects of R. sulfidophilum KKMI01 on the growth performance and gene expression were examined using 200-ton outdoor aquaria experiments. Gene expression levels were examined using qRT-PCR. Results of the short-term experiments showed the upregulation of several molting-related genes, including cuticle proteins, calcification proteins, and cuticle pigment protein, suggesting that PNSB stimulated the growth of shrimp. The upregulation of several immune genes, such as prophenoloxidase, antimicrobial peptides, and superoxide dismutase, was also observed. In the 145-day outdoor experiments, the average body weight at harvest time, survival rate, and feed conversion ratio were significantly improved in PNSB-treated shrimp, and upregulation of molting and immune-related genes were also observed. When PNSB cells were added to the rearing water, the effective dosage of PNSB was as low as 103 cfu/mL, which was more than a million times dilution of the original PNSB culture (2–3 × 109 cfu/mL), indicating that R. sulfidophilum KKMI01 provides a feasible and cost-effective application as a probiotic candidate in shrimp aquaculture.

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Litopenaeus vannamei heat shock protein 70 (LvHSP70) enhances resistance to a strain of Vibrio parahaemolyticus, which can cause acute hepatopancreatic necrosis disease (AHPND), by activating shrimp immunity

Cited by 34 publications

References 30 publications

Sustaining induced heat shock protein 70 confers biological thermotolerance in a high‐temperature adapted predatory mite Neoseiulus barkeri (Hughes)

Sustaining induced heat shock protein 70 confers biological thermotolerance in a high‐temperature adapted predatory mite Neoseiulus barkeri (Hughes)

Sustaining induced heat shock protein 70 confers biological thermotolerance: a case in a high-temperature adapted predatory mite

Probiotic Effects of a Marine Purple Non-Sulfur Bacterium, Rhodovulum sulfidophilum KKMI01, on Kuruma Shrimp (Marsupenaeus japonicus)

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