Litterbox—A gnotobiotic Zeolite-Clay System to Investigate Arabidopsis–Microbe Interactions

Abstract

Figure S1: Optimisation of the Litterbox system. A. Effect of zeolite amount on plant weight (fresh weight aboveground plant parts) of six-week-old plants grown on coarse (35 g or 70 g) zeolite covered by fine ground (20 g) zeolite. B. Effect of zeolite granularity on plant weight of six-week-old plants grown on 90 g of zeolite (70 + 20 g and 90 g). C. Effect of MES buffer (2.5 mM) on plant weight of six-week-old plants grown on coarse zeolite (70 g) covered by fine ground zeolite (20 g). Tukey's boxplots. Figure S2: Effect of agar concentration and tip size of agar filled pipette tips on plant growth. A. Plant weight of six-week-old plants (fresh weight aboveground plant parts) of plants sown either on cut 10 μl or 200 μl pipette tips filled with either 0.6% or 1% agar. Seedlings were transferred seven days after sowing to 70 g coarse zeolite covered by 20 g fine ground zeolite. B. Percentage of plants grown to full size per transplanted seedling. Filled circles represent sample mean, error bars depict standard deviation, dots mark individual plants, thick bar represents median, dotted bars represent quartiles, two-way ANOVA.

From Microbial Dynamics to Functionality in the Rhizosphere: A Systematic Review of the Opportunities With Synthetic Microbial Communities

“…For microbial effects on germination, in Moronta-Barrios et al ( 2018 ), seeds were grown in liquid LB medium, and 4 days after germination, individual seedlings were transferred to tubes containing semisolid, half-strength Hoagland solution to study the resultant inoculated-plant phenotype. Among the plant-growth systems, some others were also utilized, such as a hydroponic system (Voges et al, 2019 ); the FlowPot, which includes an autoclaved and a washed substrate such as peat (Durán et al, 2018 ; Kremer et al, 2018 ); or the Litterbox system, which uses zeolite covered by a polydimethylsiloxane sheet (Miebach et al, 2020 ). Each of these systems was selected to control different variables and to pursue different objectives.…”

“…While the first condition is free of any living microorganism (germ-free), the second one means “free of strangers” and may contain only deliberately inoculated microorganisms (Luckey, 1963 ). The gnotobiotic condition means an environment in which all the organisms, including microorganisms, are known by the investigator, whereas the last one implies undefined microbiota (Miebach et al, 2020 ).…”

Plant protoplast-based assay to screen for salicylic acid response-modulating bacteria

“…Two biological replicates were sampled per SA concentration. RNA extraction, cDNA synthesis and RT-qPCR was performed as previously described in Miebach et al (2020). Primers that were used in this study are listed in Table 2.…”

Gnotobiotic Evaluation of <em>Dalbergia sissoo</em> Genotypes for Resistance against <em>Fusarium solani</em> via Dual Culture Set up

“…Callus of genotypes of D. sissoo along with Fsd can be grown gnotobiotically under in vitro conditions in dual culture setup and functional effects of host and microbe interaction can be analyzed. This further help to understand mechanistic effect of host D. sissoo genotypes against Fsd [34]. Thus, in vitro screening in dual culture setup facilitates estimation of resistance of desirable host genotypes [35].…”