Urban green spaces (UGSs) provide numerous ecosystem services (ESs) that are essential to the well-being of the residents. However, these services are often neglected in regional urban development and spatial planning. This study quantified the ESs of a 10.25 ha UGS at Chungnam National University, Daejeon, Republic of Korea, comprising 27 species with 287 tree individuals, using i-Tree Eco. Key regulating ESs investigated included air pollution removal, carbon storage and sequestration, oxygen production, energy use reduction, avoidance of surface runoff, and replacement and functional values. Results revealed significant annual environmental benefits: 131 kg air pollutants removed (USD 3739.01 or ₩5.16 M), 1.76 Mg carbon sequestered, which is equivalent to 0.18 Mg CO2 ha−1 yr−1 (USD 289.85 or ₩0.40 M), 2.42 Mg oxygen produced, energy savings (including carbon offset) valued at USD 391.29 (₩0.54 M), and 203 m3 reduction in surface runoff (USD 413.09 or ₩0.57 M). The annual total benefits of these urban trees amounted to USD 4833.86 (₩6.67 M), USD 16.83/tree, or USD 0.089/capita. Additionally, these trees had replacement and functional values estimated at USD 311,115.17 (₩429.3 M). The study underscores that species selection and abundance of urban trees are fundamental for maximizing the ES delivery in urban areas, highlighting the role of UGSs in ecological and economical sustainability in cities. These insights are valuable for urban planners and policymakers to optimize benefits of UGSs in cities.