Live birth rates in the first complete IVF cycle among 20 687 women using a freeze-all strategy

Zhu, Qianqian; Chen, Qiuju; Wang, Li; Lü, Xuefeng; Lyu, Qifeng; Wang, Yun

doi:10.1093/humrep/dey044

Cited by 92 publications

(66 citation statements)

References 19 publications

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“…This phenomenon can be explained by a possible asynchrony between the embryo and the endometrium, a fact that was not detected in egg‐donor cycles or embryo thaw cycles (Richter, Shipley, McVearry, Tucker, & Widra, ; Shapiro, Daneshmand, Garner, Aguirre, & Ross, ). Such evidence has led to the practice of cryopreservation of all oocytes or embryos of the cohort and transfer in a subsequent cycle, free of the action of gonadotrophins, the so‐called freeze‐all policy (Chang et al, ; Roque, ; Roque, Valle, Guimaraes, Sampaio, & Geber, ), with the possibility of leading to superior results (Braga et al, ; Chang et al, ; Magdi et al, ; Roque, ; Roque et al, ; Roque, Valle, Guimaraes, Sampaio, & Geber, ; Shapiro, Daneshmand, Garner, Aguirre, & Hudson, ; Zhu et al, ). In addition, the freeze‐all policy allows the personalized embryo transfer, at the defined moment of the WOI and the stage of development of the embryo, after access to the stage of endometrial development.…”

Section: Discussionmentioning

confidence: 99%

Lipidomic profile as a noninvasive tool to predict endometrial receptivity

Braga

Borges

Godoy

et al. 2018

Molecular Reproduction Devel

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For the present study we asked whether the endometrial fluid lipidomic may be a useful approach to predict endometrial receptivity in freeze‐all cycles. For this case‐control study, endometrial fluid samples were collected from 41 patients undergoing freeze‐all cycles. Samples were split depending on the pregnancy outcome: positive group (n = 24) and negative group (n = 17). Data were acquired by the matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS‐DA) were applied. A list of potential biomarker ion ratios was obtained and the values were used to build a receiver operating characteristic (ROC) curve to predict pregnancy success. The lipid categories were attributed by LIPID MAPS database. Ion ratios were established according to their correlations and used for the analysis. The PCA showed a tendency of separation between the studied groups, whereas the PLS‐DA was able to clearly distinguish them. Fifteen ratios (13 hyper‐represented in the negative and two hyper‐represented in the positive group) were selected according to their importance for model prediction. These ratios were used to build the ROC curve, which presented an area under curve of 84.0% (95%CI: 69.2–97.4%; p = 0.009). These findings suggest that lipidomic profiling of endometrial fluid may be a valuable tool for identifying the time interval comprising the window of implantation.

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Section: Discussionmentioning

confidence: 99%

Lipidomic profile as a noninvasive tool to predict endometrial receptivity

Braga

Borges

Godoy

et al. 2018

Molecular Reproduction Devel

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“…To further improve IVF outcomes, it has been suggested to freeze all the embryos obtained in a stimulation cycle and then plan a deferred transfer during a natural cycle or with hormone replacement with exogenous estradiol (E2) and progesterone (P) for endometrial priming [105,106]. With this strategy, the frozen-thawed embryos are transferred into a more "physiological milieu" which seems to improve implantation and outcomes compared to fresh transfer [106][107][108].…”

Section: Future Perspective: the Freeze-all Strategymentioning

confidence: 99%

“…A recent retrospective study using the general population has reported that 50.74% of patients using the freeze-all strategy achieved a live birth after the first complete cycle [105]. Additionally, another study indicated positive results in poor ovarian responders, and suggested the freeze-all strategy as an alternative to cycle cancellation for these patients [110].…”

Section: Future Perspective: the Freeze-all Strategymentioning

confidence: 99%

“…Additionally, another study indicated positive results in poor ovarian responders, and suggested the freeze-all strategy as an alternative to cycle cancellation for these patients [110]. Despite these positive results, large multi-centre randomised controlled trials are needed to evaluate the freeze-all strategy [105,110].…”

Section: Future Perspective: the Freeze-all Strategymentioning

confidence: 99%

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Clinical Outcomes of Assisted Reproductive Techniques Using Cryopreserved Gametes and Embryos in Human Medicine

Waterstone¹,

Anastácio²,

Rodriguez‐Wallberg³

2018

Cryopreservation Biotechnology in Biomedical and Biological Sciences

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The methods of cryopreservation play a key role in assisted reproductive technique (ART) treatments, as they increase the efficacy of the treatments by allowing banking of supernumerary embryos for later use. It has been recently proposed that these methods could also increase the safety of ART treatments, by reducing complications such as ovarian hyperstimulation during early pregnancy; thus, the policy of total freeze for later differed transfer of embryos has been proposed. Also of great importance, cryopreservation of oocytes and spermatozoa has permitted gamete storage for long term facilitating practical routines such as the gamete banking for third-party reproductive treatment. In this chapter, the clinical indications and treatment outcomes will be revised and data updated on the safety of using cryopreservation methods in ART treatments.

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“…Since the first live birth conceived using frozen‐thawed embryos three decades ago (Trounson & Mohr, ), significant progress has been made in the cryopreservation techniques used in assisted reproductive technology (ART). The switch from slow programmed frozen methods to current vitrification methods has greatly improved ART efficiency (Zhu et al, ). Thus, freezing embryos has become a routine practice for ART units, and some units have recently even started to use the “freeze‐all” strategy for certain patients (Bhattacharya, ; Roque, Valle, Sampaio, & Geber, ).…”

Section: Introductionmentioning

confidence: 99%

Changes in DNA methylation and imprinting disorders in E9.5 mouse fetuses and placentas derived from vitrified eight‐cell embryos

Wen

et al. 2019

Molecular Reproduction Devel

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Vitrification is increasingly used in assisted reproductive technology (ART) laboratories worldwide, and potential vitrification‐induced risks require further exploration. The effect of vitrification on changes in DNA methylation and imprinting disorders was investigated in E9.5 mouse fetuses and placentas. Fetus and placental tissues were collected from the natural mating (nautural conception [NC]) group, in vitro culture (IVC) group and vitrified embryo transfer (VET) group. The fetal crown‐rump length at E9.5 in both the IVC (0.210 ± 0.059 mm) and VET (0.205 ± 0.048 mm) groups was significantly reduced compared with the NC group (0.288 ± 0.083 mm). The global methylation levels of fetuses were decreased in the IVC group compared with the NC group and it was increased after vitrification compared with IVC (p < 0.05), similar to what was observed in the NC group (p > 0.05). The changes could be attributed to the disorders of DNA methyltransferases and ten‐eleven translocations. In the IVC and VET fetuses, a majority of maternally expressed genes were upregulated, which repressed fetal growth. Furthermore, vitrification led to a change in the methylation level of KvDMR1, which resulted in the disturbance of gene imprinting. According to our results, vitrification could contribute to increased methylation compared with IVC and contributes to a gene imprinting disorder rather than recovery. Despite the routine use of embryo vitrification in clinical settings, the effect that this procedure may have on genomic imprinting deserves much greater attention.

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Live birth rates in the first complete IVF cycle among 20 687 women using a freeze-all strategy

Abstract: This work was supported by grants from the National Natural Science Foundation of China (NSFC) (31770989 to Y.W.) and the Shanghai Ninth People's Hospital Foundation of China (JYLJ030 to Y.W.). None of the authors have any conflicts of interest to declare.

Cited by 92 publications

References 19 publications

Lipidomic profile as a noninvasive tool to predict endometrial receptivity

Lipidomic profile as a noninvasive tool to predict endometrial receptivity

Clinical Outcomes of Assisted Reproductive Techniques Using Cryopreserved Gametes and Embryos in Human Medicine

Changes in DNA methylation and imprinting disorders in E9.5 mouse fetuses and placentas derived from vitrified eight‐cell embryos

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