Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies

Bui, Michelle; Shyong, Jennifer; Lutz, Eleanor K.; Yang, Ting; Li, Ming; Truong, Kenneth; Arvidson, Ryan; Buchman, Anna; Riffell, Jeffrey A.; Akbari, Omar S.

doi:10.1186/s12868-019-0511-y

Cited by 25 publications

(37 citation statements)

References 83 publications

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“…To address this issue, we next turned to functional imaging, using an Ae. aegypti knockin strain (PUb-GCaMP6s), which expresses the calciumsensitive reporter GCaMP6s from the ubiquitin locus [20] (Figure S1C). PUb-GCaMP6s mosquitoes were glued to holders that permitted two-photon imaging of calcium responses in the antennal lobe (AL) [21] ( Figures 3B and 3C).…”

Section: Two-photon Imaging Reveals Robust Neural Coding Of Geosminmentioning

confidence: 99%

Geosmin Attracts Aedes aegypti Mosquitoes to Oviposition Sites

et al. 2020

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Section: Two-photon Imaging Reveals Robust Neural Coding Of Geosminmentioning

confidence: 99%

Geosmin Attracts Aedes aegypti Mosquitoes to Oviposition Sites

et al. 2020

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“…increpitus and our PUb-GCaMP6s line of Ae. aegypti mosquitoes (20,21). Although both calcium indicators do not allow explicit recording of specific cell types in the AL, they do provide an ability to record and characterize the responses of individual glomeruli to odor stimuli.…”

Section: Divergent Mosquitoes Show Similar Antennal and Behavioralmentioning

confidence: 99%

The olfactory basis of orchid pollination by mosquitoes

Lahondère

Vinauger

Okubo

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Mosquitoes are important vectors of disease and require sources of carbohydrates for reproduction and survival. Unlike host-related behaviors of mosquitoes, comparatively less is understood about the mechanisms involved in nectar-feeding decisions, or how this sensory information is processed in the mosquito brain. Here we show that Aedes spp. mosquitoes, including Aedes aegypti, are effective pollinators of the Platanthera obtusata orchid, and demonstrate this mutualism is mediated by the orchid's scent and the balance of excitation and inhibition in the mosquito's antennal lobe (AL). The P. obtusata orchid emits an attractive, nonanal-rich scent, whereas related Platanthera species-not visited by mosquitoesemit scents dominated by lilac aldehyde. Calcium imaging experiments in the mosquito AL revealed that nonanal and lilac aldehyde each respectively activate the LC2 and AM2 glomerulus, and remarkably, the AM2 glomerulus is also sensitive to N,N-diethylmeta-toluamide (DEET), a mosquito repellent. Lateral inhibition between these 2 glomeruli reflects the level of attraction to the orchid scents. Whereas the enriched nonanal scent of P. obtusata activates the LC2 and suppresses AM2, the high level of lilac aldehyde in the other orchid scents inverts this pattern of glomerular activity, and behavioral attraction is lost. These results demonstrate the ecological importance of mosquitoes beyond operating as disease vectors and open the door toward understanding the neural basis of mosquito nectar-seeking behaviors.Platanthera | mosquitoes | Aedes aegypti | olfaction | nectar M osquitoes are important vectors of disease, such as dengue, malaria, or Zika, and are considered one of the deadliest animal on earth (1); for this reason, research has largely focused on mosquito-host interactions, and in particular, the mosquito's sensory responses to those hosts (2-5). Nectar feeding is one such aspect of mosquito sensory biology that has received comparatively less attention, despite being an excellent system in which to probe the neural bases of behavior (6). For instance, nectar and sugar feeding is critically important for both male and female mosquitoes, serving to increase their lifespan, survival rate, and reproduction, and for males, it is required for survival (6, 7).Mosquitoes are attracted to, and feed on, a variety of plant nectar sources, including those from flowers (8)(9)(10)(11)(12). Although most examples of mosquito-plant interactions have shown that mosquitoes contribute little in reproductive services to the plant (13), there are examples of mosquitoes being potential pollinators (9, 10, 14-17). However, few studies have identified the floral cues that serve to attract and mediate these decisions by the mosquitoes and how these behaviors influence pollination.The association between the Platanthera obtusata orchid and Aedes mosquitoes is one of the few examples that shows mosquitoes as effective pollinators (14-17) and thus provides investigators a unique opportunity to identify the sensory mechanisms th...

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“…Odor-evoked responses were imaged in the AL of female Ae. aegypti expressing genetically-encoded polyubiquitin-GCaMP6s during tethered flight 31 . Calcium responses were visualized on a Prairie Ultima IV multiphoton microscope (Prairie Technologies) and Ti-Sapphire laser (Chameleon Ultra, Coherent).…”

Section: -Photon Calcium Imagingmentioning

confidence: 99%

Neuromodulation and Differential Learning Across Mosquito Species

Wolff

Lahondère

Vinauger

et al. 2019

Preprint

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Mosquitoes can learn to change their host-feeding behaviors, such as shifting activity times to avoid bednets or switching from biting animals to biting humans, leading to the transfer of zoonotic diseases. Dopamine is critical for insect learning, but its role in the antennal lobe remains unclear, and it is unknown whether different mosquito species learn the same odor cues. We assayed aversive olfactory learning and dopaminergic brain innervation in four mosquito species with different host preferences and report here that they differentially learn odors salient to their preferred host and innervation patterns vary across species. Using genetically-encoded GCaMP6s Aedes aegypti, we mapped odor-evoked antennal lobe activity and report that glomeruli tuned to “learnable” odors have significantly higher dopaminergic innervation. Changes in dopamine expression in the antennal lobes of diverse invertebrate species may be an evolutionary mechanism to adapt olfactory learning circuitry without changing brain structure and for mosquitoes an ability to adapt to other hosts when their preferred are no longer present.

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Live calcium imaging of Aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies

Cited by 25 publications

References 83 publications

Geosmin Attracts Aedes aegypti Mosquitoes to Oviposition Sites

Geosmin Attracts Aedes aegypti Mosquitoes to Oviposition Sites

The olfactory basis of orchid pollination by mosquitoes

Neuromodulation and Differential Learning Across Mosquito Species

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