Live-cell analyses of transport complex formation with FRET

Majoul, Irina; Goroshkov, Alexander V.; Butkevich, Eugenia; Verrier, Sophie; Duden, Rainer

doi:10.1117/12.479842

Cited by 1 publication

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“…Recent advances in imaging technology have enabled real-time monitoring of biochemical processes in cell biology, including protein trafficking, organelle dynamics, and cell lineage or moving within a developing organism etc . The development of various laser scanning microscopy (LSM) techniques such as fluorescence resonance energy transfer (FRET) imaging, fluorescence recovery after photobleaching (FRAP), fluorescence loss in photobleaching (FLIP), and fluorescence lifetime imaging (FLIM) has enabled the study of molecular interactions and mobility of biomolecules in live cells. One such advanced imaging technique that is finding a wide range of applications in biology is “optical highlighting” .…”

Section: Introductionmentioning

confidence: 99%

Intraparticle Energy Transfer and Fluorescence Photoconversion in Nanoparticles: An Optical Highlighter Nanoprobe for Two-Photon Bioimaging

et al. 2007

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We report organically modified silica nanoparticles co-encapsulating two-photon fluorescent dye aggregates and a red-fluorescent energy acceptor, where the latter is indirectly excited through intraparticle energy transfer following two-photon excitation of the former. In vitro two-photon microscopy has demonstrated that these nanoprobes are avidly internalized into cells with bright acceptor fluorescence and that cellular optical highlighting is effected with red-to-green photoconversion by selective acceptor bleaching, while keeping the donor and the nanostructure of particles intact.

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