Live cell imaging of the intracellular compartmentalization of the contaminate benzo[a]pyrene

Abstract

This study investigates the cellular response of murine hepatoma cells to the polycyclic aromatic hydrocarbon benzo[a]pyrene (B[a]P) using two-photon and confocal laser scanning microscopy. The intracellular distribution of B[a]P and the B[a]P/AhR complex was visualized time- and concentration-dependent for up to 48 h of exposure. B[a]P was predominantly found in lipid droplets, endoplasmic reticulum and lysosomes, where B[a]P is collected and forms large aggregates. Changes in mitochondrial membrane potential and bleb formation due to high B[a]P concentrations were observed. The imaging data presented in this study provide new insights into the systemic cellular regulation following B[a]P exposure.

Mechanisms involved in the death of steatotic WIF-B9 hepatocytes co-exposed to benzo[a]pyrene and ethanol: a possible key role for xenobiotic metabolism and nitric oxide

“…6E Then, another issue about the key role of NO in the cytotoxicity of B[a]P/ethanol co-exposure in steatotic cells was addressed concerning the reduction of CYP1 activity (Fig. 2F), leading to a decrease in B[a]P metabolism ( Fig.3; note that a change in B[a]P partitioning due to the presence of lipid droplets [67,68] cannot as yet be ruled out with regard to this reduced metabolism). Indeed it is known that NO is capable of binding the heme of cytochrome P450s, thereby leading to their inhibition [62], thus suggesting a possible role for NO in the reduced activity in our model.…”

Modern trends in biophotonics for clinical diagnosis and therapy to solve unmet clinical needs

“…Two photon and confocal laser scanning microscopy investigated the cellular response of cells to polycyclic aromatic hydrocarbons . Their intracellular distributions were visualized time‐ and concentration‐dependent in live cells for up to 48 hours of exposure.…”

Revisiting the cellular toxicity of benzo[a]pyrene from the view of nanoclusters: size- and nanoplastic adsorption-dependent bioavailability

“…This spectral difference has been used for the characterization of in vivo or in vitro metabolic behaviors of Bap by means of scanning the whole wavelength for fluorescence. [49][50][51] Based on this, the whole wavelength scanning for the fluorescence of the cell (380-500 nm) was exploited to characterize the Bap metabolism behaviors, relying on the maximum fluorescence peak position difference of Bap (400 nm) and the metabolites (430 nm and 460 nm).…”