Live-Cell Pyrophosphate Imaging by in Situ Hot-Spot Generation

Li, Mingmin; Li, Jin; Di, Huixia; Liu, Huiqiao; Liu, Dingbin

doi:10.1021/acs.analchem.6b04786

Cited by 44 publications

(27 citation statements)

References 38 publications

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“…Cells were cultured in RPMI 1640 medium (GIBCO) supplemented with 10% fetal bovine serum (FBS, GIBCO) and penicillin/streptomycin (1%, 100 μg/mL). The MTT assay was applied to assess cytotoxicity as reported elsewhere with minor modifications 24. MCF-7 cells were seeded in a 96-well plate at a density of 5×10 4 cells/mL and cultured for 24 h. The adherent cells were then treated with varied concentrations of the as-prepared probes (0, 0.0125, 0.025, 0.05, 0.1, and 0.2 nM) for 24 h. The solution was replaced with 190 µL of fresh medium and 10 µL of MTT (5 mg/mL) and incubated at 37 °C for 4 h. The dark blue formazan crystals were dissolved in 150 µL of dimethylsulfoxide and the absorbance at 490 nm was measured using a microplate reader.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, new Raman reporters with high spectral resolution and a lack of background interference must be synthesized. We and other groups have shown that several exogenous moieties, including alkynes, nitriles, azides, and deuterium, show single narrow vibrational peaks in the Raman-silent region (1800-2800 cm -1 ) 24-29, where the signals of biospecies are negligible, making these chemical groups highly suitable for multiplex detection.…”

Section: Introductionmentioning

confidence: 99%

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Alkyne- and Nitrile-Anchored Gold Nanoparticles for Multiplex SERS Imaging of Biomarkers in Cancer Cells and Tissues

Li¹,

Wu²,

Ma³

et al. 2019

Nanotheranostics

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Surface-enhanced Raman spectroscopy (SERS) has proven a powerful tool for multiplex detection and imaging due to its narrow peak width and high sensitivity. However, conventional SERS reporters are limited to thiolated compounds, which have limitations such as chemical stability and spectral overlap. Here, we used alkyne- and nitrile-bearing molecules to directly fabricate a set of SERS tags for multiplex imaging. The alkyne and nitrile groups act as both the anchoring points to interact with gold nanoparticle (AuNP) surfaces and the reporters exhibiting strong and nonoverlapping signals in the cellular Raman-silent region. The SERS tags were subsequently modified with different antibodies for multicolor imaging of cancer cells and human breast cancer tissues. The reporters have a simple and readily accessible structure, hence providing new opportunities to prepare SERS nanoprobes

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Alkyne- and Nitrile-Anchored Gold Nanoparticles for Multiplex SERS Imaging of Biomarkers in Cancer Cells and Tissues

Li¹,

Wu²,

Ma³

et al. 2019

Nanotheranostics

Self Cite

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“…尽管 [151] 和(B)覆有印迹层的金纳米棒 SERS 传感器 [152] Figure 12 Overview of (A) molecularly imprinted plasmonic nanosensor for selective SERS detection of transferrin [151] and (B) gold nanorods coated with imprinted layer (MIP@AuNR) SERS sensor [152] [156] 提出了利用 C≡N 基团在拉曼静默区 (1800～2800 cm -1 )引入内标, 可有效地消除背景干扰. 王树等 [157] 合成了一种含 C≡C 三键的聚合物纳米颗粒, 在静默区也产生了较强的信号可作为内标, 降低细胞拉曼成像时的背景干扰.…”

Section: 化学学报unclassified

Advances in Protein Biomarker Assay via the Combination of Molecular Imprinting and Surface-enhanced Raman Scattering

He¹,

Zhou²,

Liu³

2021

Acta Chimica Sinica

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Abnormal protein expression closely correlates with the occurrence and development of diseases. Thus, proteins have been widely used as disease markers for early diagnosis, treatment monitoring and prognosis evaluation of diseases. However, protein biomarkers in clinical samples are usually present in extremely low concentration while the detection often suffers from severe interference by high-abundance sample matrix, which challenges the specificity and sensitivity of protein biomarker assay methods. Currently, the main detection method of protein biomarkers is immunoassay. Nevertheless, immunoassay mainly relies on antibodies for specific recognition, and antibodies are associated with disadvantages such as difficulty in preparation, poor stability, and high-cost. Meanwhile, immunoassay is mainly based on fluorescence and chemiluminescence to achieve high-sensitivity detection, but they have inherent defects such as cumbersome operation, photobleaching and broad spectrum. Molecularly imprinted polymers (MIP) have developed as biomimetic recognition materials with antibody-comparable specificity and affinity, while offering advantages such as ease in preparation, good stability and low cost. On the other hand, surface-enhanced Raman scattering (SERS) has been widely used in chemical or biological assays due to its merits including ultra-high sensitivity, narrow spectrum, speed, non-destructivity, and so on. In recent years, the combination of molecular imprinting and SERS has generated a series of advanced protein detection methods that exhibited unique strengths, which has gained wide attention. This review aims to survey the main advances of this hybrid analytical technique. After introduction of MIP and SERS as well as their separate applications in the detection of protein biomarkers, we mainly focus on the combination of MIP and SERS as well as its application in protein biomarker detection. We finally briefly sketch the future development of this hybrid analytical method.

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“…Until now, a variety of different techniques have been devoted to conducting PPi detection, including fluorescence spectroscopy [ 5 , 6 , 7 ], surface-enhanced Raman-scattering (SERS) assays [ 8 ], colorimetric sensing [ 9 , 10 , 11 ], chemiluminescence [ 12 ], electrochemical analysis [ 13 , 14 ], and dark-field optical microscopy (DFM) [ 3 ]. The principle of most of the above methods is based on the complexation of PPi with metal ions (Al 3+ [ 15 ], Fe 3+ [ 9 , 16 ], Ca 2+ [ 11 ], Zn 2+ [ 17 ], and Cu 2+ [ 5 , 6 , 10 , 12 , 13 , 18 ]), as PPi is a good complexing agent.…”

Section: Introductionmentioning

confidence: 99%

Copper (II) Ion-Modified Gold Nanoclusters as Peroxidase Mimetics for the Colorimetric Detection of Pyrophosphate

Shi

Wang

et al. 2021

Sensors

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Copper (II) ions have been shown to greatly improve the chemical stability and peroxidase-like activity of gold nanoclusters (AuNCs). Since the affinity between Cu2+ and pyrophosphate (PPi) is higher than that between Cu2+ and AuNCs, the catalytic activity of AuNCs-Cu2+ decreases with the introduction of PPi. Based on this principle, a new colorimetric detection method of PPi with high sensitivity and selectivity was developed by using AuNCs-Cu2+ as a probe. Under optimized conditions, the detection limit of PPi was 0.49 nM with a linear range of 0.51 to 30,000 nM. The sensitivity of the method was three orders of magnitude higher than that of a fluorescence method using AuNCs-Cu2+ as the probe. Finally, the AuNCs-Cu2+ system was successfully applied to directly determine the concentration of PPi in human urine samples.

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Live-Cell Pyrophosphate Imaging by in Situ Hot-Spot Generation

Cited by 44 publications

References 38 publications

Alkyne- and Nitrile-Anchored Gold Nanoparticles for Multiplex SERS Imaging of Biomarkers in Cancer Cells and Tissues

Alkyne- and Nitrile-Anchored Gold Nanoparticles for Multiplex SERS Imaging of Biomarkers in Cancer Cells and Tissues

Advances in Protein Biomarker Assay via the Combination of Molecular Imprinting and Surface-enhanced Raman Scattering

Copper (II) Ion-Modified Gold Nanoclusters as Peroxidase Mimetics for the Colorimetric Detection of Pyrophosphate

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